##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
BalI BbeI BbsI BcgI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 494 ACGTGT A^C[GA][CT]GT
AgeI 686 ACCGGT A^CCGGT
AviII 79 TGCGCA TGC^GCA
AvrII 1449 CCTAGG C^CTAGG
BamHI 842 GGATCC G^GATCC
BciVI 1048 GGATAC GTATCC(6/5)
BlnI 1449 CCTAGG C^CTAGG
BsaWI 686 ACCGGT [AT]^CCGG[AT]
BsrBI 1441 GAGCGG CCGCTC(-3/-3)
BsrDI 717 CATTGC GCAATG(2/0)
BssHII 345 GCGCGC G^CGCGC
BstYI 842 GGATCC [GA]^GATC[CT]
EarI 294 GAAGAG CTCTTC(1/4)
FspI 79 TGCGCA TGC^GCA
HgaI 999 GCGTC GACGC(5/10)
HincII 883 GTCAAC GT[CT]^[GA]AC
HindII 883 GTCAAC GT[CT]^[GA]AC
Ksp632I 294 GAAGAG CTCTTC(1/4)
KspI 28 CCGCGG CCGC^GG
MboII 294 GAAGA GAAGA(8/7)
MflI 842 GGATCC [GA]^GATC[CT]
MseI 1669 TTAA T^TAA
NcoI 1618 CCATGG C^CATGG
NheI 11 GCTAGC G^CTAGC
PinAI 686 ACCGGT A^CCGGT
PshAI 439 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1168 CAGCTG CAG^CTG
SacI 1039 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SapI 294 GAAGAGC GCTCTTC(1/4)
Tru9I 1669 TTAA T^TAA
XhoII 842 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 29 CGCG CG^CG
346 CGCG
AocI 1434 CCTGAGG CC^T[ACGT]AGG
1533 CCTGAGG
AspHI 658 GTGCTC G[AT]GC[AT]^C
1039 GAGCTC
BbrPI 493 CACGTG CAC^GTG
649 CACGTG
BbvCI 1153 GCTGAGG CCTCAGC(-5/-2)
1583 GCTGAGG
BsaAI 493 CACGTG [CT]AC^GT[GA]
649 CACGTG
BsiHKAI 658 GTGCTC G[AT]GC[AT]^C
1039 GAGCTC
BsrFI 227 ACCGGC [GA]^CCGG[CT]
686 ACCGGT
BstUI 29 CGCG CG^CG
346 CGCG
Bsu36I 1434 CCTGAGG CC^T[ACGT]AGG
1533 CCTGAGG
Cfr10I 227 ACCGGC [GA]^CCGG[CT]
686 ACCGGT
DpnII 180 GATC ^GATC
843 GATC
EagI 1025 CGGCCG C^GGCCG
1407 CGGCCG
EclXI 1025 CGGCCG C^GGCCG
1407 CGGCCG
Eco52I 1025 CGGCCG C^GGCCG
1407 CGGCCG
Eco81I 1434 CCTGAGG CC^T[ACGT]AGG
1533 CCTGAGG
HaeII 366 GGCGCT [GA]GCGC^[CT]
1336 AGCGCC
HphI 109 TCACC GGTGA(8/7)
1109 TCACC
MaeII 494 ACGT A^CGT
650 ACGT
MboI 180 GATC ^GATC
843 GATC
MvnI 29 CGCG CG^CG
346 CGCG
NdeII 180 GATC ^GATC
843 GATC
PaeR7I 1346 CTCGAG C^TCGAG
1425 CTCGAG
PmaCI 493 CACGTG CAC^GTG
649 CACGTG
PmlI 493 CACGTG CAC^GTG
649 CACGTG
PstI 271 CTGCAG CTGCA^G
433 CTGCAG
Sau3AI 180 GATC ^GATC
843 GATC
SmaI 47 CCCGGG CCC^GGG
88 CCCGGG
StuI 1452 AGGCCT AGG^CCT
1509 AGGCCT
XhoI 1346 CTCGAG C^TCGAG
1425 CTCGAG
XmaCI 47 CCCGGG C^CCGGG
88 CCCGGG
XmaI 47 CCCGGG C^CCGGG
88 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeIII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau96I
ScrFI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I