##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BamHI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI Sse8387I SspBI SspI
StuI SwaI Tru9I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 144 TCCGGA T^CCGGA
AcsI 1522 GAATTT [GA]^AATT[CT]
Alw44I 950 GTGCAC G^TGCAC
AlwI 1477 GATCC GGATC(4/5)
Aor51HI 1327 AGCGCT AGC^GCT
ApaLI 950 GTGCAC G^TGCAC
ApoI 1522 GAATTT [GA]^AATT[CT]
AspHI 950 GTGCAC G[AT]GC[AT]^C
AspI 1741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 345 CCTAGG C^CTAGG
BbsI 217 GTCTTC GAAGAC(2/6)
BbvCI 1816 GCTGAGG CCTCAGC(-5/-2)
BcgI 893 GCACCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 345 CCTAGG C^CTAGG
BpuAI 217 GTCTTC GAAGAC(2/6)
BseAI 144 TCCGGA T^CCGGA
BsiHKAI 950 GTGCAC G[AT]GC[AT]^C
BsmBI 1028 GAGACG CGTCTC(1/5)
BsmFI 242 GGGAC GGGAC(10/14)
BsmI 1881 GCATTC GAATGC(1/-1)
BspEI 144 TCCGGA T^CCGGA
BspMI 332 ACCTGC ACCTGC(4/8)
BssSI 1699 CACGAG CACGAG(-5/-1)
BstYI 1476 AGATCC [GA]^GATC[CT]
Eco47III 1327 AGCGCT AGC^GCT
EcoNI 552 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MflI 1476 AGATCC [GA]^GATC[CT]
MroI 144 TCCGGA T^CCGGA
NaeI 313 GCCGGC GCC^GGC
NcoI 68 CCATGG C^CATGG
NgoMIV 313 GCCGGC G^CCGGC
PflFI 1741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1210 GAAGAGC GCTCTTC(1/4)
SfaNI 751 GATGC GCATC(5/9)
SmaI 192 CCCGGG CCC^GGG
SpeI 1177 ACTAGT A^CTAGT
SphI 1787 GCATGC GCATG^C
TaqI 1714 TCGA T^CGA
Tth111I 1741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1714 TCGA T^CGA
XhoII 1476 AGATCC [GA]^GATC[CT]
XmaCI 192 CCCGGG C^CCGGG
XmaI 192 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 418 GGCGCC G[GA]^CG[CT]C
524 GGCGCC
AocI 52 CCTGAGG CC^T[ACGT]AGG
849 CCTGAGG
BalI 1637 TGGCCA TGG^CCA
1726 TGGCCA
BbeI 418 GGCGCC GGCGC^C
524 GGCGCC
BbrPI 703 CACGTG CAC^GTG
783 CACGTG
BglI 49 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1728 GCCAACATGGC
BsaAI 703 CACGTG [CT]AC^GT[GA]
783 CACGTG
BsaHI 418 GGCGCC G[GA]^CG[CT]C
524 GGCGCC
BsaI 1188 GAGACC GGTCTC(1/5)
1716 GAGACC
BsaWI 144 TCCGGA [AT]^CCGG[AT]
774 ACCGGA
BsiEI 311 CGGCCG CG[GA][CT]^CG
789 CGGCCG
BsrI 653 ACTGG ACTGG(1/-1)
1162 CCAGT
BstXI 1668 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1801 CCAGCTACTTGG
Bsu36I 52 CCTGAGG CC^T[ACGT]AGG
849 CCTGAGG
DsaI 68 CCATGG C^C[GA][CT]GG
392 CCACGG
EagI 311 CGGCCG C^GGCCG
789 CGGCCG
EarI 634 GAAGAG CTCTTC(1/4)
1210 GAAGAG
EclXI 311 CGGCCG C^GGCCG
789 CGGCCG
Eco52I 311 CGGCCG C^GGCCG
789 CGGCCG
Eco81I 52 CCTGAGG CC^T[ACGT]AGG
849 CCTGAGG
HgaI 103 GACGC GACGC(5/10)
148 GACGC
Hin1I 418 GGCGCC G[GA]^CG[CT]C
524 GGCGCC
HphI 1302 GGTGA GGTGA(8/7)
1336 GGTGA
KasI 418 GGCGCC G^GCGCC
524 GGCGCC
Ksp632I 634 GAAGAG CTCTTC(1/4)
1210 GAAGAG
MaeIII 229 GTCAC ^GT[ACGT]AC
1165 GTGAC
MluNI 1637 TGGCCA TGG^CCA
1726 TGGCCA
MscI 1637 TGGCCA TGG^CCA
1726 TGGCCA
NarI 418 GGCGCC GG^CGCC
524 GGCGCC
NspI 878 GCATGT [GA]CATG^[CT]
1787 GCATGC
PleI 1907 GACTC GAGTC(4/5)
1041 GAGTC
PmaCI 703 CACGTG CAC^GTG
783 CACGTG
PmlI 703 CACGTG CAC^GTG
783 CACGTG
PpuMI 165 AGGACCT [GA]G^G[AT]CC[CT]
1425 AGGACCC
PvuII 664 CAGCTG CAG^CTG
1067 CAGCTG
SfoI 418 GGCGCC GGC^GCC
524 GGCGCC
TfiI 267 GATTC G^A[AT]TC
613 GATTC
Tsp45I 229 GTCAC ^GT[GC]AC
1165 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp509I TspRI