##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII BalI BbeI BbsI
BciVI BfrI BglII BlpI Bpu1102I
BpuAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstZ17I Bsu36I CelII
ClaI DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 67 TCCGGA T^CCGGA
AcsI 407 GAATTC [GA]^AATT[CT]
Aor51HI 399 AGCGCT AGC^GCT
ApoI 407 GAATTC [GA]^AATT[CT]
AseI 1676 ATTAAT AT^TAAT
AsnI 1676 ATTAAT AT^TAAT
AvaI 482 CTCGAG C^[CT]CG[GA]G
AvrII 1638 CCTAGG C^CTAGG
BamHI 49 GGATCC G^GATCC
BbrPI 1187 CACGTG CAC^GTG
BcgI 34 CGAAGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1780 TGATCA T^GATCA
BglI 449 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1638 CCTAGG C^CTAGG
BsaAI 1187 CACGTG [CT]AC^GT[GA]
BsaBI 236 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 67 TCCGGA [AT]^CCGG[AT]
BseAI 67 TCCGGA T^CCGGA
BsmI 1793 GCATTC GAATGC(1/-1)
BsoBI 482 CTCGAG C^[CT]CG[GA]G
BspEI 67 TCCGGA T^CCGGA
BsrDI 1789 CATTGC GCAATG(2/0)
BstBI 311 TTCGAA TT^CGAA
BstEII 1252 GGTCACC G^GT[ACGT]ACC
BstPI 1252 GGTCACC G^GT[ACGT]ACC
BstXI 240 CCAATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 224 CGGACCG CG^G[AT]CCG
DraI 1661 TTTAAA TTT^AAA
Eco47III 399 AGCGCT AGC^GCT
EcoO65I 1252 GGTCACC G^GT[ACGT]ACC
EcoRI 407 GAATTC G^AATTC
FbaI 1780 TGATCA T^GATCA
MaeII 1188 ACGT A^CGT
MamI 236 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 67 TCCGGA T^CCGGA
NspI 1470 GCATGT [GA]CATG^[CT]
NspV 311 TTCGAA TT^CGAA
PaeR7I 482 CTCGAG C^TCGAG
PmaCI 1187 CACGTG CAC^GTG
PmlI 1187 CACGTG CAC^GTG
PshBI 1676 ATTAAT AT^TAAT
RsrII 224 CGGACCG CG^G[AT]CCG
SfuI 311 TTCGAA TT^CGAA
SspI 593 AATATT AAT^ATT
XcmI 1017 CCAGCGCATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 482 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 222 CGCG CG^CG
1087 CGCG
AfaI 373 GTAC GT^AC
1405 GTAC
AlwNI 383 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
920 CAGGTTCTG
ApaI 155 GGGCCC GGGCC^C
467 GGGCCC
BanI 58 GGTGCC G^G[CT][GA]CC
427 GGCACC
BbvCI 296 GCTGAGG CCTCAGC(-5/-2)
1037 GCTGAGG
BspMI 1165 GCAGGT ACCTGC(4/8)
1225 GCAGGT
BsrBI 228 CCGCTC CCGCTC(-3/-3)
1124 GAGCGG
BstUI 222 CGCG CG^CG
1087 CGCG
DraIII 702 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1070 CACAAAGTG
DsaI 646 CCGTGG C^C[GA][CT]GG
1596 CCACGG
EarI 35 GAAGAG CTCTTC(1/4)
497 GAAGAG
HaeII 399 AGCGCT [GA]GCGC^[CT]
834 AGCGCC
Ksp632I 35 GAAGAG CTCTTC(1/4)
497 GAAGAG
MslI 754 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1471 CATGTCTGTG
MvnI 222 CGCG CG^CG
1087 CGCG
NaeI 424 GCCGGC GCC^GGC
1098 GCCGGC
NgoMIV 424 GCCGGC G^CCGGC
1098 GCCGGC
PstI 380 CTGCAG CTGCA^G
389 CTGCAG
RsaI 373 GTAC GT^AC
1405 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII