##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI Asp700I AspEI AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsgI BsiEI BsiWI BsmFI BsmI
BspDI BspLU11I BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KasI KspI MluI MslI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI Sse8387I StuI SwaI TfiI
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 783 GGTACC G^GTACC
AccIII 23 TCCGGA T^CCGGA
AcyI 19 GGCGTC G[GA]^CG[CT]C
ApaI 1018 GGGCCC GGGCC^C
AseI 1446 ATTAAT AT^TAAT
AsnI 1446 ATTAAT AT^TAAT
Asp718I 783 GGTACC G^GTACC
AspHI 186 GAGCAC G[AT]GC[AT]^C
AspI 579 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 412 CCCGGG C^[CT]CG[GA]G
AviII 460 TGCGCA TGC^GCA
BalI 523 TGGCCA TGG^CCA
BbsI 804 GTCTTC GAAGAC(2/6)
BlpI 917 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 917 GCTCAGC GC^T[ACGT]AGC
BpuAI 804 GTCTTC GAAGAC(2/6)
BsaBI 315 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 19 GGCGTC G[GA]^CG[CT]C
BsaI 584 GGTCTC GGTCTC(1/5)
BsaWI 23 TCCGGA [AT]^CCGG[AT]
BseAI 23 TCCGGA T^CCGGA
BsiHKAI 186 GAGCAC G[AT]GC[AT]^C
BsoBI 412 CCCGGG C^[CT]CG[GA]G
BspEI 23 TCCGGA T^CCGGA
BssSI 189 CACGAG CACGAG(-5/-1)
BstF5I 314 GGATG GGATG(2/0)
CelII 917 GCTCAGC GC^T[ACGT]AGC
FokI 314 GGATG GGATG(9/13)
FspI 460 TGCGCA TGC^GCA
Hin1I 19 GGCGTC G[GA]^CG[CT]C
HindIII 1273 AAGCTT A^AGCTT
KpnI 783 GGTACC GGTAC^C
MaeII 811 ACGT A^CGT
MamI 315 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1795 CAATTG C^AATTG
MluNI 523 TGGCCA TGG^CCA
MroI 23 TCCGGA T^CCGGA
MscI 523 TGGCCA TGG^CCA
MspA1I 1051 CCGCTG C[AC]G^C[GT]G
MunI 1795 CAATTG C^AATTG
NcoI 787 CCATGG C^CATGG
PflFI 579 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 159 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1446 ATTAAT AT^TAAT
PstI 1000 CTGCAG CTGCA^G
SapI 1365 GCTCTTC GCTCTTC(1/4)
SfaNI 922 GCATC GCATC(5/9)
SmaI 412 CCCGGG CCC^GGG
SpeI 1508 ACTAGT A^CTAGT
SphI 427 GCATGC GCATG^C
SspI 1083 AATATT AAT^ATT
Tsp45I 1210 GTGAC ^GT[GC]AC
Tth111I 579 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 625 CCACCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 412 CCCGGG C^CCGGG
XmaI 412 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 339 CGCG CG^CG
443 CGCG
AcsI 1418 GAATTT [GA]^AATT[CT]
1614 GAATTT
AlwNI 492 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1208 CAGTGACTG
Aor51HI 720 AGCGCT AGC^GCT
842 AGCGCT
ApoI 1418 GAATTT [GA]^AATT[CT]
1614 GAATTT
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
905 CCTCAGC
BfaI 450 CTAG C^TAG
1509 CTAG
BpmI 375 CTGGAG CTGGAG(16/14)
846 CTGGAG
BsmBI 335 GAGACG CGTCTC(1/5)
392 GAGACG
Bsp1407I 734 TGTACA T^GTACA
1608 TGTACA
BspMI 457 ACCTGC ACCTGC(4/8)
706 ACCTGC
BsrBI 915 CCGCTC CCGCTC(-3/-3)
417 GAGCGG
BsrGI 734 TGTACA T^GTACA
1608 TGTACA
BstUI 339 CGCG CG^CG
443 CGCG
DsaI 787 CCATGG C^C[GA][CT]GG
1028 CCACGG
EaeI 523 TGGCCA [CT]^GGCC[GA]
715 CGGCCA
Eco47III 720 AGCGCT AGC^GCT
842 AGCGCT
EcoNI 284 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
458 CCTGCGCAAGG
EcoT14I 137 CCAAGG C^C[AT][AT]GG
787 CCATGG
FauI 911 CCCGC CCCGC(4/6)
642 GCGGG
HphI 1174 TCACC GGTGA(8/7)
1341 TCACC
MaeI 450 CTAG C^TAG
1509 CTAG
MaeIII 1210 GTGAC ^GT[ACGT]AC
1571 GTTAC
MvnI 339 CGCG CG^CG
443 CGCG
NspI 427 GCATGC [GA]CATG^[CT]
694 ACATGC
SexAI 580 ACCTGGT A^CC[AT]GGT
634 ACCTGGT
SspBI 734 TGTACA T^GTACA
1608 TGTACA
StyI 137 CCAAGG C^C[AT][AT]GG
787 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspHI BsrI BssKI BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RcaI RsaI Sau3AI
Sau96I ScrFI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII