##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbsI        BbvCI       BcgI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco81I      EcoRI       EcoT22I     FspI        
HincII      HindII      HpaI        KpnI        KspI        
MamI        MfeI        MroI        MslI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI         1996  GTAC                 GT^AC
AflIII        200  ACGCGT               A^C[GA][CT]GT
AlwNI        1289  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1907  AGCGCT               AGC^GCT
AscI          226  GGCGCGCC             GG^CGCGCC
BalI         1596  TGGCCA               TGG^CCA
BbrPI         679  CACGTG               CAC^GTG
BclI         1430  TGATCA               T^GATCA
BfaI          626  CTAG                 C^TAG
BglI          263  GCCCGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         679  CACGTG               [CT]AC^GT[GA]
BsgI          445  CTGCAC               GTGCAG(16/14)
BspHI        1427  TCATGA               T^CATGA
BsrI         1838  ACTGG                ACTGG(1/-1)
BstEII        684  GGTGACC              G^GT[ACGT]ACC
BstPI         684  GGTGACC              G^GT[ACGT]ACC
DraII         260  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII        679  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           41  CTCTTC               CTCTTC(1/4)
Eco47III     1907  AGCGCT               AGC^GCT
EcoO109I      260  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       684  GGTGACC              G^GT[ACGT]ACC
EcoRV        1423  GATATC               GAT^ATC
EcoT14I       489  CCAAGG               C^C[AT][AT]GG
FbaI         1430  TGATCA               T^GATCA
HindIII       592  AAGCTT               A^AGCTT
Ksp632I        41  CTCTTC               CTCTTC(1/4)
MaeI          626  CTAG                 C^TAG
MluI          200  ACGCGT               A^CGCGT
MluNI        1596  TGGCCA               TGG^CCA
MscI         1596  TGGCCA               TGG^CCA
PmaCI         679  CACGTG               CAC^GTG
PmlI          679  CACGTG               CAC^GTG
RcaI         1427  TCATGA               T^CATGA
RsaI         1996  GTAC                 GT^AC
SapI           40  GCTCTTC              GCTCTTC(1/4)
SbfI          252  CCTGCAGG             CCTGCA^GG
ScaI         1995  AGTACT               AGT^ACT
SfaNI        1328  GCATC                GCATC(5/9)
Sse8387I      252  CCTGCAGG             CCTGCA^GG
StyI          489  CCAAGG               C^C[AT][AT]GG
XcmI         1828  CCAGGATGGGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AatII        1315  GACGTC               GACGT^C
             1369  GACGTC              
ApaI          191  GGGCCC               GGGCC^C
              261  GGGCCC              
Asp700I       580  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1267  GAAGTAATTC          
AvaII        1218  GGACC                G^G[AT]CC
             1404  GGTCC               
BciVI        1172  GTATCC               GTATCC(6/5)
             1678  GTATCC              
BseRI         828  CTCCTC               GAGGAG(10/8)
             1748  CTCCTC              
EcoNI         252  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1936  CCTCTTTTAGG         
FseI           99  GGCCGGCC             GGCCGG^CC
              339  GGCCGGCC            
SmlI         1303  CTCAAG               C^T[CT][GA]AG
             1551  CTCAAG              
TaqI          578  TCGA                 T^CGA
             1791  TCGA                
TthHB8I       578  TCGA                 T^CGA
             1791  TCGA                
XmnI          580  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1267  GAAGTAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        BanI        BanII       BbeI        
BbvI        BcnI        BmyI        BsaHI       BsaI        
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrFI       BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EagI        EclXI       Eco52I      EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfcI        
SfoI        SmaI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmaCI       
XmaI