##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoRI EcoT22I FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MroI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 1996 GTAC GT^AC
AflIII 200 ACGCGT A^C[GA][CT]GT
AlwNI 1289 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1907 AGCGCT AGC^GCT
AscI 226 GGCGCGCC GG^CGCGCC
BalI 1596 TGGCCA TGG^CCA
BbrPI 679 CACGTG CAC^GTG
BclI 1430 TGATCA T^GATCA
BfaI 626 CTAG C^TAG
BglI 263 GCCCGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 679 CACGTG [CT]AC^GT[GA]
BsgI 445 CTGCAC GTGCAG(16/14)
BspHI 1427 TCATGA T^CATGA
BsrI 1838 ACTGG ACTGG(1/-1)
BstEII 684 GGTGACC G^GT[ACGT]ACC
BstPI 684 GGTGACC G^GT[ACGT]ACC
DraII 260 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 679 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 41 CTCTTC CTCTTC(1/4)
Eco47III 1907 AGCGCT AGC^GCT
EcoO109I 260 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 684 GGTGACC G^GT[ACGT]ACC
EcoRV 1423 GATATC GAT^ATC
EcoT14I 489 CCAAGG C^C[AT][AT]GG
FbaI 1430 TGATCA T^GATCA
HindIII 592 AAGCTT A^AGCTT
Ksp632I 41 CTCTTC CTCTTC(1/4)
MaeI 626 CTAG C^TAG
MluI 200 ACGCGT A^CGCGT
MluNI 1596 TGGCCA TGG^CCA
MscI 1596 TGGCCA TGG^CCA
PmaCI 679 CACGTG CAC^GTG
PmlI 679 CACGTG CAC^GTG
RcaI 1427 TCATGA T^CATGA
RsaI 1996 GTAC GT^AC
SapI 40 GCTCTTC GCTCTTC(1/4)
SbfI 252 CCTGCAGG CCTGCA^GG
ScaI 1995 AGTACT AGT^ACT
SfaNI 1328 GCATC GCATC(5/9)
Sse8387I 252 CCTGCAGG CCTGCA^GG
StyI 489 CCAAGG C^C[AT][AT]GG
XcmI 1828 CCAGGATGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AatII 1315 GACGTC GACGT^C
1369 GACGTC
ApaI 191 GGGCCC GGGCC^C
261 GGGCCC
Asp700I 580 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1267 GAAGTAATTC
AvaII 1218 GGACC G^G[AT]CC
1404 GGTCC
BciVI 1172 GTATCC GTATCC(6/5)
1678 GTATCC
BseRI 828 CTCCTC GAGGAG(10/8)
1748 CTCCTC
EcoNI 252 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1936 CCTCTTTTAGG
FseI 99 GGCCGGCC GGCCGG^CC
339 GGCCGGCC
SmlI 1303 CTCAAG C^T[CT][GA]AG
1551 CTCAAG
TaqI 578 TCGA T^CGA
1791 TCGA
TthHB8I 578 TCGA T^CGA
1791 TCGA
XmnI 580 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1267 GAAGTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfcI
SfoI SmaI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI