################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoT22I FspI HincII HindII HpaI KpnI KspI MamI MfeI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 1996 GTAC GT^AC AflIII 200 ACGCGT A^C[GA][CT]GT AlwNI 1289 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1907 AGCGCT AGC^GCT AscI 226 GGCGCGCC GG^CGCGCC BalI 1596 TGGCCA TGG^CCA BbrPI 679 CACGTG CAC^GTG BclI 1430 TGATCA T^GATCA BfaI 626 CTAG C^TAG BglI 263 GCCCGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 679 CACGTG [CT]AC^GT[GA] BsgI 445 CTGCAC GTGCAG(16/14) BspHI 1427 TCATGA T^CATGA BsrI 1838 ACTGG ACTGG(1/-1) BstEII 684 GGTGACC G^GT[ACGT]ACC BstPI 684 GGTGACC G^GT[ACGT]ACC DraII 260 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 679 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 41 CTCTTC CTCTTC(1/4) Eco47III 1907 AGCGCT AGC^GCT EcoO109I 260 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 684 GGTGACC G^GT[ACGT]ACC EcoRV 1423 GATATC GAT^ATC EcoT14I 489 CCAAGG C^C[AT][AT]GG FbaI 1430 TGATCA T^GATCA HindIII 592 AAGCTT A^AGCTT Ksp632I 41 CTCTTC CTCTTC(1/4) MaeI 626 CTAG C^TAG MluI 200 ACGCGT A^CGCGT MluNI 1596 TGGCCA TGG^CCA MscI 1596 TGGCCA TGG^CCA PmaCI 679 CACGTG CAC^GTG PmlI 679 CACGTG CAC^GTG RcaI 1427 TCATGA T^CATGA RsaI 1996 GTAC GT^AC SapI 40 GCTCTTC GCTCTTC(1/4) SbfI 252 CCTGCAGG CCTGCA^GG ScaI 1995 AGTACT AGT^ACT SfaNI 1328 GCATC GCATC(5/9) Sse8387I 252 CCTGCAGG CCTGCA^GG StyI 489 CCAAGG C^C[AT][AT]GG XcmI 1828 CCAGGATGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AatII 1315 GACGTC GACGT^C 1369 GACGTC ApaI 191 GGGCCC GGGCC^C 261 GGGCCC Asp700I 580 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1267 GAAGTAATTC AvaII 1218 GGACC G^G[AT]CC 1404 GGTCC BciVI 1172 GTATCC GTATCC(6/5) 1678 GTATCC BseRI 828 CTCCTC GAGGAG(10/8) 1748 CTCCTC EcoNI 252 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1936 CCTCTTTTAGG FseI 99 GGCCGGCC GGCCGG^CC 339 GGCCGGCC SmlI 1303 CTCAAG C^T[CT][GA]AG 1551 CTCAAG TaqI 578 TCGA T^CGA 1791 TCGA TthHB8I 578 TCGA T^CGA 1791 TCGA XmnI 580 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1267 GAAGTAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI SfoI SmaI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmaCI XmaI