##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BglI BglII BlnI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DpnII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MamI MboI MflI MluI MluNI
MroI MscI MslI MspA1I NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 897 AACGTT AA^CGTT
AcyI 76 GACGCC G[GA]^CG[CT]C
Aor51HI 1648 AGCGCT AGC^GCT
AvaI 1695 CCCGAG C^[CT]CG[GA]G
AvaII 1641 GGACC G^G[AT]CC
BbsI 593 GAAGAC GAAGAC(2/6)
BcnI 481 CCGGG CC^[GC]GG
BlpI 1202 GCTGAGC GC^T[ACGT]AGC
BpmI 1652 CTGGAG CTGGAG(16/14)
Bpu1102I 1202 GCTGAGC GC^T[ACGT]AGC
BpuAI 593 GAAGAC GAAGAC(2/6)
BsaAI 521 TACGTG [CT]AC^GT[GA]
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsaI 1169 GGTCTC GGTCTC(1/5)
BsaWI 1026 TCCGGT [AT]^CCGG[AT]
BsgI 1864 CTGCAC GTGCAG(16/14)
BsiEI 116 CGGTCG CG[GA][CT]^CG
BsmBI 1655 GAGACG CGTCTC(1/5)
BsoBI 1695 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1774 TGTACA T^GTACA
BspLU11I 1610 ACATGT A^CATGT
BspMI 1245 ACCTGC ACCTGC(4/8)
BsrGI 1774 TGTACA T^GTACA
BstEII 406 GGTAACC G^GT[ACGT]ACC
BstPI 406 GGTAACC G^GT[ACGT]ACC
BstXI 1275 CCAACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1202 GCTGAGC GC^T[ACGT]AGC
DraI 1524 TTTAAA TTT^AAA
DraIII 1064 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1648 AGCGCT AGC^GCT
EcoNI 1261 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 406 GGTAACC G^GT[ACGT]ACC
HaeII 1648 AGCGCT [GA]GCGC^[CT]
HgaI 76 GACGC GACGC(5/10)
Hin1I 76 GACGCC G[GA]^CG[CT]C
HindIII 236 AAGCTT A^AGCTT
MfeI 397 CAATTG C^AATTG
MunI 397 CAATTG C^AATTG
NciI 481 CCGGG CC^[GC]GG
PshAI 107 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 897 AACGTT AA^CGTT
PstI 13 CTGCAG CTGCA^G
SfcI 13 CTGCAG C^T[GA][CT]AG
SspBI 1774 TGTACA T^GTACA
SspI 1563 AATATT AAT^ATT
TfiI 52 GATTC G^A[AT]TC
Tsp45I 1817 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 2 CGCG CG^CG
27 CGCG
AflII 172 CTTAAG C^TTAAG
230 CTTAAG
AflIII 1610 ACATGT A^C[GA][CT]GT
1820 ACGTGT
AspHI 1205 GAGCAC G[AT]GC[AT]^C
1698 GAGCAC
BbvCI 340 CCTCAGC CCTCAGC(-5/-2)
512 GCTGAGG
BbvI 64 GCTGC GCAGC(8/12)
1663 GCTGC
BfrI 172 CTTAAG C^TTAAG
230 CTTAAG
BmyI 1205 GAGCAC G[AGT]GC[ACT]^C
1698 GAGCAC
BsaJI 1192 CCCAGG C^C[ACGT][ACGT]GG
1281 CCCTGG
BsiHKAI 1205 GAGCAC G[AT]GC[AT]^C
1698 GAGCAC
Bsp1286I 1205 GAGCAC G[AGT]GC[ACT]^C
1698 GAGCAC
BstUI 2 CGCG CG^CG
27 CGCG
Cac8I 1260 GCCTGC GC[ACGT]^[ACGT]GC
1443 GCAGGC
EcoRI 167 GAATTC G^AATTC
1600 GAATTC
Fnu4HI 64 GCTGC GC^[ACGT]GC
1663 GCTGC
HapII 481 CCGG C^CGG
1027 CCGG
HpaII 481 CCGG C^CGG
1027 CCGG
ItaI 64 GCTGC GC^[ACGT]GC
1663 GCTGC
MspI 481 CCGG C^CGG
1027 CCGG
MvnI 2 CGCG CG^CG
27 CGCG
MwoI 445 GCTCCTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1271 GCATCCAACGC
NspI 1610 ACATGT [GA]CATG^[CT]
1666 GCATGT
SapI 710 GAAGAGC GCTCTTC(1/4)
1891 GAAGAGC
TseI 64 GCTGC G^C[AT]GC
1663 GCTGC
TspRI 357 CACTG CA[GC]TG[ACGT][ACGT]^
1319 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BfaI BseRI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DraII EarI
EcoO109I EcoRII FauI FokI HaeIII
HhaI HinP1I HinfI HphI Ksp632I
MaeI MaeII MaeIII MboII MnlI
MseI MvaI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfaNI SmlI
StuI TaqI Tru9I Tsp509I TthHB8I