##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI AhdI AluI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI MvaI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 67 CGCG CG^CG
AclI 1254 AACGTT AA^CGTT
AflII 1491 CTTAAG C^TTAAG
Asp700I 606 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 760 GTGCTC G[AT]GC[AT]^C
AvaI 109 CCCGGG C^[CT]CG[GA]G
AvaII 569 GGACC G^G[AT]CC
BalI 18 TGGCCA TGG^CCA
BamHI 632 GGATCC G^GATCC
BanI 113 GGTGCC G^G[CT][GA]CC
BanII 595 GAGCCC G[GA]GC[CT]^C
BfrI 1491 CTTAAG C^TTAAG
BglII 728 AGATCT A^GATCT
BsaAI 1592 TACGTA [CT]AC^GT[GA]
BsiHKAI 760 GTGCTC G[AT]GC[AT]^C
BsmAI 1686 GTCTC GTCTC(1/5)
BsmI 1735 GAATGC GAATGC(1/-1)
BsoBI 109 CCCGGG C^[CT]CG[GA]G
Bsp1407I 753 TGTACA T^GTACA
BspMI 852 ACCTGC ACCTGC(4/8)
BsrBI 1 GAGCGG CCGCTC(-3/-3)
BsrFI 73 GCCGGC [GA]^CCGG[CT]
BsrGI 753 TGTACA T^GTACA
BstUI 67 CGCG CG^CG
Cac8I 73 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 136 GCGC GCG^C
Cfr10I 73 GCCGGC [GA]^CCGG[CT]
Cfr13I 569 GGACC G^G[ACGT]CC
DsaI 277 CCATGG C^C[GA][CT]GG
EaeI 18 TGGCCA [CT]^GGCC[GA]
EarI 1402 GAAGAG CTCTTC(1/4)
HgaI 68 GCGTC GACGC(5/10)
HhaI 136 GCGC GCG^C
HinP1I 136 GCGC G^CGC
Ksp632I 1402 GAAGAG CTCTTC(1/4)
MluNI 18 TGGCCA TGG^CCA
MscI 18 TGGCCA TGG^CCA
MspA1I 82 CAGCGG C[AC]G^C[GT]G
MvnI 67 CGCG CG^CG
NaeI 73 GCCGGC GCC^GGC
NcoI 277 CCATGG C^CATGG
NgoMIV 73 GCCGGC G^CCGGC
Psp1406I 1254 AACGTT AA^CGTT
Sau96I 569 GGACC G^G[ACGT]CC
SmaI 109 CCCGGG CCC^GGG
SnaBI 1592 TACGTA TAC^GTA
SspBI 753 TGTACA T^GTACA
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
XmnI 606 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 633 GATCC GGATC(4/5)
632 GGATC
AlwNI 439 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
547 CAGCCACTG
AseI 1370 ATTAAT AT^TAAT
1505 ATTAAT
AsnI 1370 ATTAAT AT^TAAT
1505 ATTAAT
BcnI 109 CCCGG CC^[GC]GG
110 CCGGG
BspHI 289 TCATGA T^CATGA
783 TCATGA
BsrDI 539 CATTGC GCAATG(2/0)
1264 CATTGC
BssKI 109 CCCGG ^CC[ACGT]GG
110 CCGGG
BstF5I 748 CATCC GGATG(2/0)
281 GGATG
BstYI 632 GGATCC [GA]^GATC[CT]
728 AGATCT
DpnII 633 GATC ^GATC
729 GATC
DraI 183 TTTAAA TTT^AAA
198 TTTAAA
FauI 39 GCGGG CCCGC(4/6)
213 GCGGG
FokI 748 CATCC GGATG(9/13)
281 GGATG
HapII 74 CCGG C^CGG
110 CCGG
HinfI 221 GAGTC G^A[ACGT]TC
247 GACTC
HpaII 74 CCGG C^CGG
110 CCGG
HphI 488 GGTGA GGTGA(8/7)
1237 GGTGA
MboI 633 GATC ^GATC
729 GATC
MflI 632 GGATCC [GA]^GATC[CT]
728 AGATCT
MspI 74 CCGG C^CGG
110 CCGG
NciI 109 CCCGG CC^[GC]GG
110 CCGGG
NdeII 633 GATC ^GATC
729 GATC
PleI 247 GACTC GAGTC(4/5)
221 GAGTC
PshBI 1370 ATTAAT AT^TAAT
1505 ATTAAT
RcaI 289 TCATGA T^CATGA
783 TCATGA
Sau3AI 633 GATC ^GATC
729 GATC
ScrFI 109 CCCGG CC^[ACGT]GG
110 CCGGG
SmlI 724 CTTGAG C^T[CT][GA]AG
1491 CTTAAG
XcmI 343 CCAAACAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
409 CCAAGGAGGGAGTGG
XhoII 632 GGATCC [GA]^GATC[CT]
728 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI ApoI BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
DdeI EcoRI EcoT14I Fnu4HI HaeIII
ItaI MaeII MaeIII MboII MnlI
MseI MslI MwoI NlaIII NlaIV
RsaI SspI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I