##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspHI AspI AviII AvrII BalI
BamHI BbsI BciVI BfrI BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1392 GGTACC G^GTACC
AcsI 1845 GAATTT [GA]^AATT[CT]
AhdI 131 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 777 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1845 GAATTT [GA]^AATT[CT]
Asp718I 1392 GGTACC G^GTACC
AspEI 131 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 603 CACGTG CAC^GTG
BbvCI 1462 CCTCAGC CCTCAGC(-5/-2)
BclI 886 TGATCA T^GATCA
BglII 196 AGATCT A^GATCT
BsaAI 603 CACGTG [CT]AC^GT[GA]
BsgI 524 CTGCAC GTGCAG(16/14)
BsiEI 1217 CGGCCG CG[GA][CT]^CG
BsmAI 1563 GTCTC GTCTC(1/5)
BspHI 1595 TCATGA T^CATGA
BspMI 1036 ACCTGC ACCTGC(4/8)
BsrBI 1381 CCGCTC CCGCTC(-3/-3)
BsrFI 793 GCCGGC [GA]^CCGG[CT]
BstAPI 776 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 205 GGTGACC G^GT[ACGT]ACC
BstPI 205 GGTGACC G^GT[ACGT]ACC
Cfr10I 793 GCCGGC [GA]^CCGG[CT]
DraIII 540 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1217 CGGCCG C^GGCCG
Eam1105I 131 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1008 GAAGAG CTCTTC(1/4)
EclXI 1217 CGGCCG C^GGCCG
Eco52I 1217 CGGCCG C^GGCCG
EcoNI 1613 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 205 GGTGACC G^GT[ACGT]ACC
FbaI 886 TGATCA T^GATCA
KpnI 1392 GGTACC GGTAC^C
Ksp632I 1008 GAAGAG CTCTTC(1/4)
MseI 769 TTAA T^TAA
NaeI 793 GCCGGC GCC^GGC
NgoMIV 793 GCCGGC G^CCGGC
PleI 1732 GAGTC GAGTC(4/5)
PmaCI 603 CACGTG CAC^GTG
PmlI 603 CACGTG CAC^GTG
PshAI 1727 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1595 TCATGA T^CATGA
SexAI 1267 ACCTGGT A^CC[AT]GGT
SmlI 819 CTCAAG C^T[CT][GA]AG
SpeI 188 ACTAGT A^CTAGT
TfiI 1868 GAATC G^A[AT]TC
Tru9I 769 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1185 GACGTC GACGT^C
1296 GACGTC
AfaI 841 GTAC GT^AC
1393 GTAC
BbeI 257 GGCGCC GGCGC^C
1194 GGCGCC
BcgI 715 GCAGCGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
871 GCAAGGCCTTCG
BglI 781 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
910 GCCGCCCTGGC
BlpI 285 GCTGAGC GC^T[ACGT]AGC
1329 GCTCAGC
Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC
1329 GCTCAGC
BssSI 1729 CACGAG CACGAG(-5/-1)
399 CTCGTG
BstXI 99 CCACACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1081 CCACCATGGTGG
BstYI 196 AGATCT [GA]^GATC[CT]
989 GGATCT
CelII 285 GCTGAGC GC^T[ACGT]AGC
1329 GCTCAGC
HaeII 257 GGCGCC [GA]GCGC^[CT]
1194 GGCGCC
HgaI 1206 GACGC GACGC(5/10)
1616 GACGC
HinfI 1732 GAGTC G^A[ACGT]TC
1868 GAATC
KasI 257 GGCGCC G^GCGCC
1194 GGCGCC
MflI 196 AGATCT [GA]^GATC[CT]
989 GGATCT
MslI 740 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1082 CACCATGGTG
NarI 257 GGCGCC GG^CGCC
1194 GGCGCC
NcoI 422 CCATGG C^CATGG
1084 CCATGG
PflMI 291 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCACAAAGTGG
RsaI 841 GTAC GT^AC
1393 GTAC
SfoI 257 GGCGCC GGC^GCC
1194 GGCGCC
SmaI 967 CCCGGG CCC^GGG
1257 CCCGGG
StuI 490 AGGCCT AGG^CCT
874 AGGCCT
Tsp509I 1502 AATT ^AATT
1846 AATT
Van91I 291 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCACAAAGTGG
XhoII 196 AGATCT [GA]^GATC[CT]
989 GGATCT
XmaCI 967 CCCGGG C^CCGGG
1257 CCCGGG
XmaI 967 CCCGGG C^CCGGG
1257 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI