##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraIII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfaNI SfcI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1320 GACGTC GACGT^C
Acc65I 991 GGTACC G^GTACC
AflIII 1344 ACGTGT A^C[GA][CT]GT
AhdI 962 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 529 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 893 GGGCCC GGGCC^C
Asp718I 991 GGTACC G^GTACC
AspEI 962 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 679 GTGCTC G[AT]GC[AT]^C
BbeI 287 GGCGCC GGCGC^C
BbsI 1224 GAAGAC GAAGAC(2/6)
BbvCI 209 CCTCAGC CCTCAGC(-5/-2)
BglI 265 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1104 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1104 GCTGAGC GC^T[ACGT]AGC
BpuAI 1224 GAAGAC GAAGAC(2/6)
BsaWI 1154 TCCGGT [AT]^CCGG[AT]
BsiEI 18 CGATCG CG[GA][CT]^CG
BsiHKAI 679 GTGCTC G[AT]GC[AT]^C
BsmBI 1322 CGTCTC CGTCTC(1/5)
BsmFI 301 GGGAC GGGAC(10/14)
Bsp1407I 407 TGTACA T^GTACA
BsrDI 928 GCAATG GCAATG(2/0)
BsrGI 407 TGTACA T^GTACA
CelII 1104 GCTGAGC GC^T[ACGT]AGC
DrdI 303 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 962 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 712 CCTACCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1085 GAATTC G^AATTC
HinfI 883 GATTC G^A[ACGT]TC
KasI 287 GGCGCC G^GCGCC
KpnI 991 GGTACC GGTAC^C
NarI 287 GGCGCC GG^CGCC
NspI 742 ACATGC [GA]CATG^[CT]
PflMI 1041 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 18 CGATCG CGAT^CG
SalI 763 GTCGAC G^TCGAC
SfiI 264 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 287 GGCGCC GGC^GCC
SspBI 407 TGTACA T^GTACA
TfiI 883 GATTC G^A[AT]TC
Tsp45I 135 GTCAC ^GT[GC]AC
Van91I 1041 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 763 GTCGAC GT^[AC][GT]AC
841 GTAGAC
AcsI 1085 GAATTC [GA]^AATT[CT]
1186 AAATTC
AlwI 1071 GATCC GGATC(4/5)
669 GGATC
ApoI 1085 GAATTC [GA]^AATT[CT]
1186 AAATTC
BanI 287 GGCGCC G^G[CT][GA]CC
991 GGTACC
BanII 52 GAGCCC G[GA]GC[CT]^C
893 GGGCCC
BseRI 898 CTCCTC GAGGAG(10/8)
105 GAGGAG
BsgI 789 GTGCAG GTGCAG(16/14)
826 GTGCAG
BstF5I 1152 CATCC GGATG(2/0)
1165 CATCC
EcoT14I 391 CCAAGG C^C[AT][AT]GG
965 CCAAGG
FokI 1152 CATCC GGATG(9/13)
1165 CATCC
HaeII 213 AGCGCC [GA]GCGC^[CT]
287 GGCGCC
HphI 136 TCACC GGTGA(8/7)
142 TCACC
PpuMI 530 AGGTCCT [GA]G^G[AT]CC[CT]
608 GGGTCCT
PvuII 114 CAGCTG CAG^CTG
1078 CAGCTG
SmaI 100 CCCGGG CCC^GGG
266 CCCGGG
StyI 391 CCAAGG C^C[AT][AT]GG
965 CCAAGG
TaqI 17 TCGA T^CGA
764 TCGA
TspRI 455 CAGTG CA[GC]TG[ACGT][ACGT]^
847 CAGTG
TthHB8I 17 TCGA T^CGA
764 TCGA
XmaCI 100 CCCGGG C^CCGGG
266 CCCGGG
XmaI 100 CCCGGG C^CCGGG
266 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
AvaII BbvI BcnI BfaI BmyI
BsaHI BsaI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp509I