##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BfrI BglI BglII BlnI
BsaAI BsaHI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrDI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MluNI
MscI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 430 GTAGAC GT^[AC][GT]AC
AccIII 1089 TCCGGA T^CCGGA
AgeI 587 ACCGGT A^CCGGT
AseI 1429 ATTAAT AT^TAAT
AsnI 1429 ATTAAT AT^TAAT
BanII 640 GAGCCC G[GA]GC[CT]^C
BbsI 202 GAAGAC GAAGAC(2/6)
BbvCI 318 GCTGAGG CCTCAGC(-5/-2)
BciVI 408 GGATAC GTATCC(6/5)
BclI 1218 TGATCA T^GATCA
BlpI 837 GCTCAGC GC^T[ACGT]AGC
BpmI 1456 CTCCAG CTGGAG(16/14)
Bpu1102I 837 GCTCAGC GC^T[ACGT]AGC
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaBI 1285 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1089 TCCGGA T^CCGGA
Bsp1407I 818 TGTACA T^GTACA
BspEI 1089 TCCGGA T^CCGGA
BspMI 178 ACCTGC ACCTGC(4/8)
BsrGI 818 TGTACA T^GTACA
BssSI 889 CACGAG CACGAG(-5/-1)
BstYI 1290 AGATCC [GA]^GATC[CT]
CelII 837 GCTCAGC GC^T[ACGT]AGC
EaeI 195 TGGCCG [CT]^GGCC[GA]
FbaI 1218 TGATCA T^GATCA
HindIII 1347 AAGCTT A^AGCTT
MaeII 662 ACGT A^CGT
MamI 1285 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1290 AGATCC [GA]^GATC[CT]
MroI 1089 TCCGGA T^CCGGA
MslI 915 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 336 GCATGC [GA]CATG^[CT]
PinAI 587 ACCGGT A^CCGGT
PshBI 1429 ATTAAT AT^TAAT
SbfI 303 CCTGCAGG CCTGCA^GG
SexAI 785 ACCAGGT A^CC[AT]GGT
SmaI 47 CCCGGG CCC^GGG
SphI 336 GCATGC GCATG^C
Sse8387I 303 CCTGCAGG CCTGCA^GG
SspBI 818 TGTACA T^GTACA
XcmI 1458 CCAGCCCCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1290 AGATCC [GA]^GATC[CT]
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1126 GAATTT [GA]^AATT[CT]
1495 AAATTT
AlwI 507 GATCC GGATC(4/5)
1291 GATCC
ApoI 1126 GAATTT [GA]^AATT[CT]
1495 AAATTT
BanI 273 GGTGCC G^G[CT][GA]CC
931 GGTGCC
BfaI 466 CTAG C^TAG
1230 CTAG
BmyI 274 GTGCCC G[AGT]GC[ACT]^C
640 GAGCCC
BsaI 1621 GAGACC GGTCTC(1/5)
862 GGTCTC
BsaWI 587 ACCGGT [AT]^CCGG[AT]
1089 TCCGGA
BseRI 1522 GAGGAG GAGGAG(10/8)
1618 GAGGAG
BsgI 595 CTGCAC GTGCAG(16/14)
517 GTGCAG
Bsp1286I 274 GTGCCC G[AGT]GC[ACT]^C
640 GAGCCC
BsrBI 1085 CCGCTC CCGCTC(-3/-3)
11 GAGCGG
BsrFI 311 ACCGGC [GA]^CCGG[CT]
587 ACCGGT
BssHII 24 GCGCGC G^CGCGC
26 GCGCGC
Cfr10I 311 ACCGGC [GA]^CCGG[CT]
587 ACCGGT
EcoNI 612 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1611 CCTTCAAGAGG
EcoT14I 33 CCATGG C^C[AT][AT]GG
633 CCATGG
MaeI 466 CTAG C^TAG
1230 CTAG
MseI 1287 TTAA T^TAA
1430 TTAA
NcoI 33 CCATGG C^CATGG
633 CCATGG
PaeR7I 601 CTCGAG C^TCGAG
995 CTCGAG
PshAI 1073 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1095 GACAGATGTC
StuI 260 AGGCCT AGG^CCT
419 AGGCCT
StyI 33 CCATGG C^C[AT][AT]GG
633 CCATGG
Tru9I 1287 TTAA T^TAA
1430 TTAA
XhoI 601 CTCGAG C^TCGAG
995 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I