##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BbsI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eco47III Eco81I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1877 GTAGAC GT^[AC][GT]AC
AhdI 365 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 365 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 423 TGGCCA TGG^CCA
BcgI 2167 CGAGGTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 806 CTAG C^TAG
BsaI 796 GGTCTC GGTCTC(1/5)
Bsp1407I 351 TGTACA T^GTACA
BsrBI 2036 CCGCTC CCGCTC(-3/-3)
BsrGI 351 TGTACA T^GTACA
DraIII 1353 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 137 GACTGTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1868 CGGCCG C^GGCCG
Eam1105I 365 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 449 CTCTTC CTCTTC(1/4)
EclXI 1868 CGGCCG C^GGCCG
Eco52I 1868 CGGCCG C^GGCCG
EcoT22I 1392 ATGCAT ATGCA^T
HindIII 2127 AAGCTT A^AGCTT
Ksp632I 449 CTCTTC CTCTTC(1/4)
MaeI 806 CTAG C^TAG
MluNI 423 TGGCCA TGG^CCA
MscI 423 TGGCCA TGG^CCA
NaeI 324 GCCGGC GCC^GGC
NcoI 39 CCATGG C^CATGG
NgoMIV 324 GCCGGC G^CCGGC
NotI 1867 GCGGCCGC GC^GGCCGC
NsiI 1392 ATGCAT ATGCA^T
PvuII 107 CAGCTG CAG^CTG
SapI 448 GCTCTTC GCTCTTC(1/4)
SgrAI 323 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 351 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 1591 GACGTC GACGT^C
2336 GACGTC
BamHI 117 GGATCC G^GATCC
2069 GGATCC
BbvCI 787 CCTCAGC CCTCAGC(-5/-2)
757 GCTGAGG
BclI 571 TGATCA T^GATCA
1349 TGATCA
BspMI 124 GCAGGT ACCTGC(4/8)
1873 GCAGGT
BsrDI 2173 CATTGC GCAATG(2/0)
2408 GCAATG
BstEII 610 GGTGACC G^GT[ACGT]ACC
1560 GGTGACC
BstPI 610 GGTGACC G^GT[ACGT]ACC
1560 GGTGACC
EcoO65I 610 GGTGACC G^GT[ACGT]ACC
1560 GGTGACC
FbaI 571 TGATCA T^GATCA
1349 TGATCA
KspI 1865 CCGCGG CCGC^GG
2013 CCGCGG
MseI 2056 TTAA T^TAA
2400 TTAA
PstI 947 CTGCAG CTGCA^G
1201 CTGCAG
SacII 1865 CCGCGG CCGC^GG
2013 CCGCGG
SfcI 947 CTGCAG C^T[GA][CT]AG
1201 CTGCAG
TfiI 391 GATTC G^A[AT]TC
2140 GATTC
Tru9I 2056 TTAA T^TAA
2400 TTAA
Tsp509I 883 AATT ^AATT
1808 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI