##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AcsI AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbsI BcgI BciVI BclI
BglI BlnI BpuAI BsaI BseAI
BsiEI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Ksp632I KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 629 GGTACC G^GTACC
AccI 355 GTCTAC GT^[AC][GT]AC
AflII 541 CTTAAG C^TTAAG
AhdI 682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 972 AGCGCT AGC^GCT
Asp718I 629 GGTACC G^GTACC
AspEI 682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 191 TGCGCA TGC^GCA
BbeI 1718 GGCGCC GGCGC^C
BbrPI 187 CACGTG CAC^GTG
BbvCI 1294 GCTGAGG CCTCAGC(-5/-2)
BfrI 541 CTTAAG C^TTAAG
BglII 498 AGATCT A^GATCT
BlpI 414 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 414 GCTGAGC GC^T[ACGT]AGC
BsaAI 187 CACGTG [CT]AC^GT[GA]
BsaBI 267 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 926 ACCGGA [AT]^CCGG[AT]
BsiWI 158 CGTACG C^GTACG
BsmI 701 GCATTC GAATGC(1/-1)
Bsp1407I 389 TGTACA T^GTACA
BsrBI 1075 GAGCGG CCGCTC(-3/-3)
BsrDI 419 GCAATG GCAATG(2/0)
BsrGI 389 TGTACA T^GTACA
BstBI 229 TTCGAA TT^CGAA
BstXI 1049 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 414 GCTGAGC GC^T[ACGT]AGC
Eam1105I 682 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 972 AGCGCT AGC^GCT
FspI 191 TGCGCA TGC^GCA
HincII 933 GTTAAC GT[CT]^[GA]AC
HindII 933 GTTAAC GT[CT]^[GA]AC
HindIII 883 AAGCTT A^AGCTT
HpaI 933 GTTAAC GTT^AAC
KasI 1718 GGCGCC G^GCGCC
KpnI 629 GGTACC GGTAC^C
MaeIII 1648 GTCAC ^GT[ACGT]AC
MamI 267 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1718 GGCGCC GG^CGCC
NcoI 1049 CCATGG C^CATGG
NheI 1596 GCTAGC G^CTAGC
NspV 229 TTCGAA TT^CGAA
PmaCI 187 CACGTG CAC^GTG
PmlI 187 CACGTG CAC^GTG
PvuII 412 CAGCTG CAG^CTG
ScaI 998 AGTACT AGT^ACT
SfcI 844 CTACAG C^T[GA][CT]AG
SfoI 1718 GGCGCC GGC^GCC
SfuI 229 TTCGAA TT^CGAA
SspBI 389 TGTACA T^GTACA
TfiI 1626 GAATC G^A[AT]TC
Tsp45I 1648 GTCAC ^GT[GC]AC
Tsp509I 811 AATT ^AATT
XcmI 860 CCAAGACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 327 GGCGTC G[GA]^CG[CT]C
1718 GGCGCC
AocI 1069 CCTAAGG CC^T[ACGT]AGG
1605 CCTGAGG
ApaI 117 GGGCCC GGGCC^C
635 GGGCCC
BalI 206 TGGCCA TGG^CCA
1276 TGGCCA
BanI 629 GGTACC G^G[CT][GA]CC
1718 GGCGCC
BsaHI 327 GGCGTC G[GA]^CG[CT]C
1718 GGCGCC
BsgI 1159 CTGCAC GTGCAG(16/14)
364 GTGCAG
BsrFI 595 ACCGGC [GA]^CCGG[CT]
725 ACCGGC
Bsu36I 1069 CCTAAGG CC^T[ACGT]AGG
1605 CCTGAGG
Cfr10I 595 ACCGGC [GA]^CCGG[CT]
725 ACCGGC
DsaI 1049 CCATGG C^C[GA][CT]GG
1349 CCACGG
Eco81I 1069 CCTAAGG CC^T[ACGT]AGG
1605 CCTGAGG
EcoNI 1065 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1472 CCTGCTGAAGG
HgaI 328 GCGTC GACGC(5/10)
1422 GCGTC
Hin1I 327 GGCGTC G[GA]^CG[CT]C
1718 GGCGCC
MluNI 206 TGGCCA TGG^CCA
1276 TGGCCA
MscI 206 TGGCCA TGG^CCA
1276 TGGCCA
MslI 292 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
819 CATCAACATG
MspA1I 412 CAGCTG C[AC]G^C[GT]G
1390 CCGCTG
PflMI 768 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAGTCTCTGG
SacI 1087 GAGCTC GAGCT^C
1363 GAGCTC
SmaI 66 CCCGGG CCC^GGG
75 CCCGGG
StuI 1298 AGGCCT AGG^CCT
1322 AGGCCT
Van91I 768 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAGTCTCTGG
XmaCI 66 CCCGGG C^CCGGG
75 CCCGGG
XmaI 66 CCCGGG C^CCGGG
75 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I TseI
TspRI TthHB8I XhoII