##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbvCI
BciVI BclI BlnI BseRI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 827 GTAGAC GT^[AC][GT]AC
AccIII 1591 TCCGGA T^CCGGA
AcsI 974 GAATTC [GA]^AATT[CT]
AflII 2575 CTTAAG C^TTAAG
AflIII 2401 ACGTGT A^C[GA][CT]GT
Alw44I 5 GTGCAC G^TGCAC
ApaI 1064 GGGCCC GGGCC^C
ApaLI 5 GTGCAC G^TGCAC
ApoI 974 GAATTC [GA]^AATT[CT]
Asp700I 2200 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 5 GTGCAC G[AT]GC[AT]^C
AviII 1908 TGCGCA TGC^GCA
BbeI 16 GGCGCC GGCGC^C
BbrPI 2400 CACGTG CAC^GTG
BcgI 1465 CGAGACCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2575 CTTAAG C^TTAAG
BsaAI 2400 CACGTG [CT]AC^GT[GA]
BsaBI 1762 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1591 TCCGGA T^CCGGA
BsiHKAI 5 GTGCAC G[AT]GC[AT]^C
BspEI 1591 TCCGGA T^CCGGA
BsrBI 1295 GAGCGG CCGCTC(-3/-3)
BssSI 2096 CACGAG CACGAG(-5/-1)
BstXI 814 CCACGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 291 CGGACCG CG^G[AT]CCG
EcoRI 974 GAATTC G^AATTC
FspI 1908 TGCGCA TGC^GCA
KasI 16 GGCGCC G^GCGCC
MamI 1762 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1591 TCCGGA T^CCGGA
NarI 16 GGCGCC GG^CGCC
PaeR7I 2017 CTCGAG C^TCGAG
PmaCI 2400 CACGTG CAC^GTG
PmlI 2400 CACGTG CAC^GTG
RsrII 291 CGGACCG CG^G[AT]CCG
SfiI 319 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 16 GGCGCC GGC^GCC
SphI 2029 GCATGC GCATG^C
TaqI 2018 TCGA T^CGA
TthHB8I 2018 TCGA T^CGA
XhoI 2017 CTCGAG C^TCGAG
XmnI 2200 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1283 CGCG CG^CG
2312 CGCG
AcyI 16 GGCGCC G[GA]^CG[CT]C
2383 GGCGTC
AvaI 1463 CCCGAG C^[CT]CG[GA]G
2017 CTCGAG
BbsI 950 GAAGAC GAAGAC(2/6)
2547 GTCTTC
BfaI 155 CTAG C^TAG
965 CTAG
BglII 1314 AGATCT A^GATCT
2571 AGATCT
BlpI 1197 GCTCAGC GC^T[ACGT]AGC
2484 GCTTAGC
Bpu1102I 1197 GCTCAGC GC^T[ACGT]AGC
2484 GCTTAGC
BpuAI 950 GAAGAC GAAGAC(2/6)
2547 GTCTTC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
2383 GGCGTC
BsaWI 728 TCCGGT [AT]^CCGG[AT]
1591 TCCGGA
BsmBI 1995 GAGACG CGTCTC(1/5)
2291 GAGACG
BsoBI 1463 CCCGAG C^[CT]CG[GA]G
2017 CTCGAG
BsrFI 192 ACCGGC [GA]^CCGG[CT]
941 ACCGGC
BstUI 1283 CGCG CG^CG
2312 CGCG
CelII 1197 GCTCAGC GC^T[ACGT]AGC
2484 GCTTAGC
Cfr10I 192 ACCGGC [GA]^CCGG[CT]
941 ACCGGC
EcoNI 1425 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1765 CCTCATCAAGG
Hin1I 16 GGCGCC G[GA]^CG[CT]C
2383 GGCGTC
MaeI 155 CTAG C^TAG
965 CTAG
MvnI 1283 CGCG CG^CG
2312 CGCG
NcoI 1228 CCATGG C^CATGG
2433 CCATGG
PvuII 1203 CAGCTG CAG^CTG
1806 CAGCTG
TfiI 525 GAATC G^A[AT]TC
2164 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BamHI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII