##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BbeI BbrPI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 607 GACGTC GACGT^C
AccI 1448 GTCTAC GT^[AC][GT]AC
AfaI 1651 GTAC GT^AC
AflIII 236 ACGTGT A^C[GA][CT]GT
AocI 507 CCTGAGG CC^T[ACGT]AGG
Asp700I 786 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 706 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 348 TGCGCA TGC^GCA
BamHI 1006 GGATCC G^GATCC
BbsI 304 GTCTTC GAAGAC(2/6)
BbvCI 157 CCTCAGC CCTCAGC(-5/-2)
BciVI 519 GTATCC GTATCC(6/5)
BfaI 565 CTAG C^TAG
BpuAI 304 GTCTTC GAAGAC(2/6)
BsgI 1564 CTGCAC GTGCAG(16/14)
BsiEI 281 CGGCCG CG[GA][CT]^CG
BsmI 786 GAATGC GAATGC(1/-1)
Bsu36I 507 CCTGAGG CC^T[ACGT]AGG
CpoI 710 CGGTCCG CG^G[AT]CCG
DraIII 526 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 281 CGGCCG C^GGCCG
EarI 769 CTCTTC CTCTTC(1/4)
EclXI 281 CGGCCG C^GGCCG
Eco52I 281 CGGCCG C^GGCCG
Eco81I 507 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1160 CCAAGG C^C[AT][AT]GG
FspI 348 TGCGCA TGC^GCA
Ksp632I 769 CTCTTC CTCTTC(1/4)
KspI 1392 CCGCGG CCGC^GG
MaeI 565 CTAG C^TAG
MslI 1499 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 706 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1350 CTGCAG CTGCA^G
RsaI 1651 GTAC GT^AC
RsrII 710 CGGTCCG CG^G[AT]CCG
SacI 1637 GAGCTC GAGCT^C
SacII 1392 CCGCGG CCGC^GG
SapI 768 GCTCTTC GCTCTTC(1/4)
SbfI 1349 CCTGCAGG CCTGCA^GG
SexAI 527 ACCTGGT A^CC[AT]GGT
SfiI 63 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1255 CCCGGG CCC^GGG
Sse8387I 1349 CCTGCAGG CCTGCA^GG
StyI 1160 CCAAGG C^C[AT][AT]GG
TfiI 1783 GATTC G^A[AT]TC
Tth111I 706 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1255 CCCGGG C^CCGGG
XmaI 1255 CCCGGG C^CCGGG
XmnI 786 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 607 GACGTC G[GA]^CG[CT]C
1538 GACGCC
BalI 803 TGGCCA TGG^CCA
2089 TGGCCA
BsaHI 607 GACGTC G[GA]^CG[CT]C
1538 GACGCC
BsaI 963 GAGACC GGTCTC(1/5)
2006 GGTCTC
BseRI 1432 CTCCTC GAGGAG(10/8)
2016 CTCCTC
BsmAI 963 GAGAC GTCTC(1/5)
2007 GTCTC
BsrFI 1488 ACCGGC [GA]^CCGG[CT]
1529 ACCGGC
BssHII 425 GCGCGC G^CGCGC
554 GCGCGC
BstXI 1042 CCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1141 CCAGCTTGGTGG
Cfr10I 1488 ACCGGC [GA]^CCGG[CT]
1529 ACCGGC
Hin1I 607 GACGTC G[GA]^CG[CT]C
1538 GACGCC
MaeII 236 ACGT A^CGT
608 ACGT
MluNI 803 TGGCCA TGG^CCA
2089 TGGCCA
MscI 803 TGGCCA TGG^CCA
2089 TGGCCA
PvuII 1501 CAGCTG CAG^CTG
2045 CAGCTG
SfaNI 454 GATGC GCATC(5/9)
492 GATGC
SmlI 2009 CTCAAG C^T[CT][GA]AG
2039 CTCAAG
TaqI 131 TCGA T^CGA
1845 TCGA
TthHB8I 131 TCGA T^CGA
1845 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
Aor51HI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI Sau3AI
Sau96I ScrFI SfcI StuI TseI
Tsp45I TspRI Van91I XcmI XhoII