##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BsiHKAI BsiWI BsmBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI BstYI CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 1282 GTATAC GT^[AC][GT]AC
AccII 246 CGCG CG^CG
AcyI 220 GACGCC G[GA]^CG[CT]C
AflII 1344 CTTAAG C^TTAAG
AlwI 712 GGATC GGATC(4/5)
Aor51HI 305 AGCGCT AGC^GCT
Asp700I 773 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 126 CCCGAG C^[CT]CG[GA]G
BalI 238 TGGCCA TGG^CCA
BanI 54 GGTGCC G^G[CT][GA]CC
BbvCI 167 CCTCAGC CCTCAGC(-5/-2)
BcnI 555 CCGGG CC^[GC]GG
BfrI 1344 CTTAAG C^TTAAG
BglI 200 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 220 GACGCC G[GA]^CG[CT]C
BseRI 1187 GAGGAG GAGGAG(10/8)
BsiEI 183 CGGTCG CG[GA][CT]^CG
BsmI 1617 GAATGC GAATGC(1/-1)
BsoBI 126 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1696 TGTACA T^GTACA
BspMI 230 ACCTGC ACCTGC(4/8)
BsrBI 224 CCGCTC CCGCTC(-3/-3)
BsrDI 209 GCAATG GCAATG(2/0)
BsrGI 1696 TGTACA T^GTACA
Bst1107I 1282 GTATAC GTA^TAC
BstUI 246 CGCG CG^CG
BstZ17I 1282 GTATAC GTA^TAC
CfoI 306 GCGC GCG^C
DraI 938 TTTAAA TTT^AAA
DraII 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1010 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 993 GACACTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 238 TGGCCA [CT]^GGCC[GA]
Eco47III 305 AGCGCT AGC^GCT
EcoO109I 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1213 GAATTC G^AATTC
EcoT14I 204 CCATGG C^C[AT][AT]GG
FauI 109 GCGGG CCCGC(4/6)
HaeII 305 AGCGCT [GA]GCGC^[CT]
HhaI 306 GCGC GCG^C
Hin1I 220 GACGCC G[GA]^CG[CT]C
HinP1I 306 GCGC G^CGC
HincII 550 GTTAAC GT[CT]^[GA]AC
HindII 550 GTTAAC GT[CT]^[GA]AC
HpaI 550 GTTAAC GTT^AAC
MaeII 1100 ACGT A^CGT
MluNI 238 TGGCCA TGG^CCA
MscI 238 TGGCCA TGG^CCA
MspA1I 435 CAGCTG C[AC]G^C[GT]G
MvnI 246 CGCG CG^CG
NciI 555 CCGGG CC^[GC]GG
NcoI 204 CCATGG C^CATGG
NdeI 1267 CATATG CA^TATG
PleI 18 GACTC GAGTC(4/5)
PvuII 435 CAGCTG CAG^CTG
SspBI 1696 TGTACA T^GTACA
StuI 1251 AGGCCT AGG^CCT
StyI 204 CCATGG C^C[AT][AT]GG
TaqI 703 TCGA T^CGA
TthHB8I 703 TCGA T^CGA
XmnI 773 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 1477 GTAC GT^AC
1697 GTAC
AlwNI 432 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1236 CAGCTCCTG
AocI 611 CCTAAGG CC^T[ACGT]AGG
782 CCTGAGG
AvaII 847 GGACC G^G[AT]CC
1001 GGTCC
BfaI 98 CTAG C^TAG
1193 CTAG
BpmI 1387 CTCCAG CTGGAG(16/14)
117 CTGGAG
BsaWI 60 ACCGGA [AT]^CCGG[AT]
181 TCCGGT
BsgI 658 GTGCAG GTGCAG(16/14)
834 GTGCAG
BsmFI 559 GGGAC GGGAC(10/14)
991 GGGAC
Bsu36I 611 CCTAAGG CC^T[ACGT]AGG
782 CCTGAGG
DsaI 204 CCATGG C^C[GA][CT]GG
252 CCGTGG
Eco81I 611 CCTAAGG CC^T[ACGT]AGG
782 CCTGAGG
HgaI 220 GACGC GACGC(5/10)
1259 GCGTC
MaeI 98 CTAG C^TAG
1193 CTAG
MslI 205 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
377 CATAAAGGTG
PflMI 453 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCAAAGATTGG
PstI 135 CTGCAG CTGCA^G
438 CTGCAG
RsaI 1477 GTAC GT^AC
1697 GTAC
SmlI 1344 CTTAAG C^T[CT][GA]AG
1436 CTCAAG
TfiI 676 GATTC G^A[AT]TC
980 GAATC
Van91I 453 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCAAAGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp509I TspRI