##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BanII BbrPI BciVI
BclI BfrI BlnI BsaAI BsaBI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BsrFI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MluI MluNI MscI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1035 GGTACC G^GTACC
AccI 704 GTAGAC GT^[AC][GT]AC
AccIII 964 TCCGGA T^CCGGA
Alw44I 873 GTGCAC G^TGCAC
ApaLI 873 GTGCAC G^TGCAC
Asp718I 1035 GGTACC G^GTACC
AspHI 873 GTGCAC G[AT]GC[AT]^C
BamHI 961 GGATCC G^GATCC
BbeI 107 GGCGCC GGCGC^C
BbsI 1214 GAAGAC GAAGAC(2/6)
BbvCI 336 GCTGAGG CCTCAGC(-5/-2)
BcgI 59 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 99 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1133 AGATCT A^GATCT
BlpI 1385 GCTCAGC GC^T[ACGT]AGC
BmyI 873 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1385 GCTCAGC GC^T[ACGT]AGC
BpuAI 1214 GAAGAC GAAGAC(2/6)
BsaI 533 GGTCTC GGTCTC(1/5)
BseAI 964 TCCGGA T^CCGGA
BsiHKAI 873 GTGCAC G[AT]GC[AT]^C
BsmI 1090 GAATGC GAATGC(1/-1)
Bsp1286I 873 GTGCAC G[AGT]GC[ACT]^C
BspEI 964 TCCGGA T^CCGGA
BspHI 988 TCATGA T^CATGA
BsrBI 1383 CCGCTC CCGCTC(-3/-3)
BsrDI 538 CATTGC GCAATG(2/0)
BssHII 37 GCGCGC G^CGCGC
BssSI 668 CTCGTG CACGAG(-5/-1)
BstXI 951 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1385 GCTCAGC GC^T[ACGT]AGC
DraI 1227 TTTAAA TTT^AAA
DraII 378 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 378 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 854 GATATC GAT^ATC
HaeII 107 GGCGCC [GA]GCGC^[CT]
HincII 400 GTTGAC GT[CT]^[GA]AC
HindII 400 GTTGAC GT[CT]^[GA]AC
HphI 818 GGTGA GGTGA(8/7)
KasI 107 GGCGCC G^GCGCC
KpnI 1035 GGTACC GGTAC^C
MaeII 1449 ACGT A^CGT
MfeI 974 CAATTG C^AATTG
MroI 964 TCCGGA T^CCGGA
MunI 974 CAATTG C^AATTG
NarI 107 GGCGCC GG^CGCC
NcoI 1152 CCATGG C^CATGG
NdeI 918 CATATG CA^TATG
PleI 728 GAGTC GAGTC(4/5)
PpuMI 378 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 988 TCATGA T^CATGA
SapI 417 GAAGAGC GCTCTTC(1/4)
ScaI 1765 AGTACT AGT^ACT
SfcI 1301 CTGTAG C^T[GA][CT]AG
SfoI 107 GGCGCC GGC^GCC
SmlI 1462 CTCAAG C^T[CT][GA]AG
XbaI 731 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 444 AAATTC [GA]^AATT[CT]
1230 AAATTT
AflIII 708 ACATGT A^C[GA][CT]GT
843 ACATGT
AlwI 962 GATCC GGATC(4/5)
961 GGATC
AlwNI 123 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1181 CAGAGACTG
ApoI 444 AAATTC [GA]^AATT[CT]
1230 AAATTT
Asp700I 458 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
980 GAAACCCTTC
BanI 107 GGCGCC G^G[CT][GA]CC
1035 GGTACC
BcnI 47 CCGGG CC^[GC]GG
356 CCGGG
BpmI 302 CTCCAG CTGGAG(16/14)
1137 CTCCAG
BsaWI 964 TCCGGA [AT]^CCGG[AT]
1032 TCCGGT
BsgI 1533 CTGCAC GTGCAG(16/14)
558 GTGCAG
BsmAI 1183 GAGAC GTCTC(1/5)
534 GTCTC
BsmFI 378 GGGAC GGGAC(10/14)
602 GTCCC
BspLU11I 708 ACATGT A^CATGT
843 ACATGT
BspMI 1012 ACCTGC ACCTGC(4/8)
1525 ACCTGC
BstYI 961 GGATCC [GA]^GATC[CT]
1133 AGATCT
EaeI 137 CGGCCA [CT]^GGCC[GA]
692 CGGCCA
EarI 280 GAAGAG CTCTTC(1/4)
417 GAAGAG
EcoT14I 1102 CCTTGG C^C[AT][AT]GG
1152 CCATGG
FauI 43 CCCGC CCCGC(4/6)
21 GCGGG
Ksp632I 280 GAAGAG CTCTTC(1/4)
417 GAAGAG
KspI 19 CCGCGG CCGC^GG
134 CCGCGG
MflI 961 GGATCC [GA]^GATC[CT]
1133 AGATCT
NciI 47 CCGGG CC^[GC]GG
356 CCGGG
PvuII 649 CAGCTG CAG^CTG
1388 CAGCTG
SacII 19 CCGCGG CCGC^GG
134 CCGCGG
StyI 1102 CCTTGG C^C[AT][AT]GG
1152 CCATGG
TaqI 230 TCGA T^CGA
807 TCGA
TthHB8I 230 TCGA T^CGA
807 TCGA
XhoII 961 GGATCC [GA]^GATC[CT]
1133 AGATCT
XmnI 458 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
980 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BfaI BsaHI BsaJI
BsiYI BslI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI