##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MaeI
MaeII MfeI MflI MluI MroI
MseI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 1004 AGCGCT AGC^GCT
Asp700I 1795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 394 TGCGCA TGC^GCA
BbsI 1875 GAAGAC GAAGAC(2/6)
BpuAI 1875 GAAGAC GAAGAC(2/6)
BsaBI 203 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1268 GAGACC GGTCTC(1/5)
BsrDI 1256 GCAATG GCAATG(2/0)
BssSI 666 CTCGTG CACGAG(-5/-1)
BstAPI 629 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 1698 GAAGAG CTCTTC(1/4)
Eco47III 1004 AGCGCT AGC^GCT
EcoRV 1455 GATATC GAT^ATC
FspI 394 TGCGCA TGC^GCA
Ksp632I 1698 GAAGAG CTCTTC(1/4)
MamI 203 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 86 CCATGG C^CATGG
NspI 1956 GCATGT [GA]CATG^[CT]
XmnI 1795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 5 GTAC GT^AC
1142 GTAC
Alw44I 627 GTGCAC G^TGCAC
1195 GTGCAC
AlwI 329 GATCC GGATC(4/5)
1729 GATCC
AlwNI 18 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1745 CAGTATCTG
ApaLI 627 GTGCAC G^TGCAC
1195 GTGCAC
BalI 95 TGGCCA TGG^CCA
277 TGGCCA
BbeI 843 GGCGCC GGCGC^C
1061 GGCGCC
BsmAI 1268 GAGAC GTCTC(1/5)
1785 GAGAC
BsrFI 572 GCCGGC [GA]^CCGG[CT]
899 GCCGGC
BstXI 86 CCATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
106 CCAAGAGGATGG
Cfr10I 572 GCCGGC [GA]^CCGG[CT]
899 GCCGGC
DpnII 329 GATC ^GATC
1729 GATC
EcoT14I 86 CCATGG C^C[AT][AT]GG
1818 CCAAGG
KasI 843 GGCGCC G^GCGCC
1061 GGCGCC
MboI 329 GATC ^GATC
1729 GATC
MluNI 95 TGGCCA TGG^CCA
277 TGGCCA
MscI 95 TGGCCA TGG^CCA
277 TGGCCA
MslI 674 CACCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
857 CATCTTCGTG
NaeI 572 GCCGGC GCC^GGC
899 GCCGGC
NarI 843 GGCGCC GG^CGCC
1061 GGCGCC
NdeII 329 GATC ^GATC
1729 GATC
NgoMIV 572 GCCGGC G^CCGGC
899 GCCGGC
NotI 456 GCGGCCGC GC^GGCCGC
650 GCGGCCGC
PflMI 135 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2031 CCATTTTCTGG
PvuII 337 CAGCTG CAG^CTG
2022 CAGCTG
RsaI 5 GTAC GT^AC
1142 GTAC
Sau3AI 329 GATC ^GATC
1729 GATC
SfaNI 856 GCATC GCATC(5/9)
1616 GCATC
SfoI 843 GGCGCC GGC^GCC
1061 GGCGCC
SmaI 501 CCCGGG CCC^GGG
978 CCCGGG
StuI 1586 AGGCCT AGG^CCT
1973 AGGCCT
StyI 86 CCATGG C^C[AT][AT]GG
1818 CCAAGG
TfiI 1528 GAATC G^A[AT]TC
1993 GATTC
Tsp509I 1021 AATT ^AATT
1979 AATT
Van91I 135 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2031 CCATTTTCTGG
XmaCI 501 CCCGGG C^CCGGG
978 CCCGGG
XmaI 501 CCCGGG C^CCGGG
978 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AluI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssHII BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI PpuMI PstI
SacII Sau96I ScrFI SfcI SfiI
TseI Tsp45I TspRI XcmI