##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BalI BbrPI BbsI BcgI
BciVI BclI BglII BlnI BpuAI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI MamI MluI
MluNI MscI MslI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1131 GGTACC G^GTACC
AccI 1154 GTAGAC GT^[AC][GT]AC
AccIII 691 TCCGGA T^CCGGA
AcsI 1765 GAATTC [GA]^AATT[CT]
AflII 1509 CTTAAG C^TTAAG
AlwNI 283 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1765 GAATTC [GA]^AATT[CT]
Asp718I 1131 GGTACC G^GTACC
AspHI 676 GAGCTC G[AT]GC[AT]^C
BbeI 1114 GGCGCC GGCGC^C
BbvCI 163 GCTGAGG CCTCAGC(-5/-2)
BfaI 126 CTAG C^TAG
BfrI 1509 CTTAAG C^TTAAG
BlpI 1395 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC
BsaI 1346 GGTCTC GGTCTC(1/5)
BsaWI 691 TCCGGA [AT]^CCGG[AT]
BseAI 691 TCCGGA T^CCGGA
BsiHKAI 676 GAGCTC G[AT]GC[AT]^C
BsmBI 304 GAGACG CGTCTC(1/5)
BspEI 691 TCCGGA T^CCGGA
BspMI 478 GCAGGT ACCTGC(4/8)
BstEII 1500 GGTTACC G^GT[ACGT]ACC
BstPI 1500 GGTTACC G^GT[ACGT]ACC
CelII 1395 GCTGAGC GC^T[ACGT]AGC
DrdI 775 GACGCGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1784 CTCTTC CTCTTC(1/4)
EcoNI 1006 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1500 GGTTACC G^GT[ACGT]ACC
EcoRI 1765 GAATTC G^AATTC
HindIII 1291 AAGCTT A^AGCTT
KasI 1114 GGCGCC G^GCGCC
KpnI 1131 GGTACC GGTAC^C
Ksp632I 1784 CTCTTC CTCTTC(1/4)
MaeI 126 CTAG C^TAG
MfeI 1602 CAATTG C^AATTG
MroI 691 TCCGGA T^CCGGA
MseI 1510 TTAA T^TAA
MunI 1602 CAATTG C^AATTG
NarI 1114 GGCGCC GG^CGCC
NspI 812 GCATGT [GA]CATG^[CT]
PstI 476 CTGCAG CTGCA^G
PvuII 1530 CAGCTG CAG^CTG
SacI 676 GAGCTC GAGCT^C
SfcI 476 CTGCAG C^T[GA][CT]AG
SfoI 1114 GGCGCC GGC^GCC
SmlI 1509 CTTAAG C^T[CT][GA]AG
Tru9I 1510 TTAA T^TAA
XcmI 278 CCAGCCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 157 CCTCAGG CC^T[ACGT]AGG
1056 CCTGAGG
BssHII 575 GCGCGC G^CGCGC
618 GCGCGC
BssSI 1305 CACGAG CACGAG(-5/-1)
1349 CTCGTG
Bsu36I 157 CCTCAGG CC^T[ACGT]AGG
1056 CCTGAGG
EaeI 53 TGGCCG [CT]^GGCC[GA]
718 TGGCCG
Eco81I 157 CCTCAGG CC^T[ACGT]AGG
1056 CCTGAGG
KspI 30 CCGCGG CCGC^GG
701 CCGCGG
MaeII 937 ACGT A^CGT
1377 ACGT
NaeI 309 GCCGGC GCC^GGC
544 GCCGGC
NcoI 671 CCATGG C^CATGG
1199 CCATGG
NgoMIV 309 GCCGGC G^CCGGC
544 GCCGGC
PflMI 282 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCAGTCAGTGG
SacII 30 CCGCGG CCGC^GG
701 CCGCGG
SgrAI 308 CGCCGGCG C[GA]^CCGG[CT]G
804 CGCCGGTG
SspI 1673 AATATT AAT^ATT
1741 AATATT
Tsp45I 915 GTCAC ^GT[GC]AC
1374 GTCAC
Van91I 282 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCAGTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Asp700I AvaI AvaII BamHI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TfiI TseI
Tsp509I TspRI XhoII XmaCI XmaI
XmnI