##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 38 CGCG CG^CG
AocI 860 CCTGAGG CC^T[ACGT]AGG
ApaI 181 GGGCCC GGGCC^C
AvaI 184 CCCGGG C^[CT]CG[GA]G
BamHI 226 GGATCC G^GATCC
BbsI 1611 GAAGAC GAAGAC(2/6)
BclI 463 TGATCA T^GATCA
BpuAI 1611 GAAGAC GAAGAC(2/6)
BsaWI 31 ACCGGA [AT]^CCGG[AT]
BsoBI 184 CCCGGG C^[CT]CG[GA]G
BsrBI 14 GAGCGG CCGCTC(-3/-3)
BsrDI 413 CATTGC GCAATG(2/0)
BstAPI 123 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1564 GGTGACC G^GT[ACGT]ACC
BstPI 1564 GGTGACC G^GT[ACGT]ACC
BstUI 38 CGCG CG^CG
Bsu36I 860 CCTGAGG CC^T[ACGT]AGG
Eco81I 860 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1564 GGTGACC G^GT[ACGT]ACC
EcoRV 1415 GATATC GAT^ATC
EcoT14I 61 CCATGG C^C[AT][AT]GG
FbaI 463 TGATCA T^GATCA
MslI 1206 CACCTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 38 CGCG CG^CG
NcoI 61 CCATGG C^CATGG
NspI 1072 GCATGT [GA]CATG^[CT]
PflMI 1898 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2046 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 243 GAAGAGC GCTCTTC(1/4)
SmaI 184 CCCGGG CCC^GGG
SspI 2040 AATATT AAT^ATT
StuI 1252 AGGCCT AGG^CCT
StyI 61 CCATGG C^C[AT][AT]GG
Van91I 1898 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1688 TCTAGA T^CTAGA
XmaCI 184 CCCGGG C^CCGGG
XmaI 184 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1498 AAATTT [GA]^AATT[CT]
1767 AAATTT
ApoI 1498 AAATTT [GA]^AATT[CT]
1767 AAATTT
AvaII 251 GGACC G^G[AT]CC
776 GGACC
BalI 271 TGGCCA TGG^CCA
1812 TGGCCA
BanI 57 GGCACC G^G[CT][GA]CC
1805 GGCACC
BanII 181 GGGCCC G[GA]GC[CT]^C
1278 GAGCCC
BbvCI 195 GCTGAGG CCTCAGC(-5/-2)
201 GCTGAGG
BstF5I 1938 CATCC GGATG(2/0)
769 GGATG
BstYI 100 AGATCC [GA]^GATC[CT]
226 GGATCC
DraI 1064 TTTAAA TTT^AAA
1764 TTTAAA
DsaI 61 CCATGG C^C[GA][CT]GG
1847 CCGTGG
EaeI 271 TGGCCA [CT]^GGCC[GA]
1812 TGGCCA
EcoT22I 1052 ATGCAT ATGCA^T
1213 ATGCAT
FauI 179 GCGGG CCCGC(4/6)
874 GCGGG
FokI 1938 CATCC GGATG(9/13)
769 GGATG
HaeII 148 AGCGCC [GA]GCGC^[CT]
265 AGCGCC
MfeI 709 CAATTG C^AATTG
1704 CAATTG
MflI 100 AGATCC [GA]^GATC[CT]
226 GGATCC
MluNI 271 TGGCCA TGG^CCA
1812 TGGCCA
MscI 271 TGGCCA TGG^CCA
1812 TGGCCA
MunI 709 CAATTG C^AATTG
1704 CAATTG
NsiI 1052 ATGCAT ATGCA^T
1213 ATGCAT
PvuII 231 CAGCTG CAG^CTG
995 CAGCTG
ScaI 592 AGTACT AGT^ACT
1324 AGTACT
SfaNI 129 GATGC GCATC(5/9)
770 GATGC
TaqI 82 TCGA T^CGA
1171 TCGA
TthHB8I 82 TCGA T^CGA
1171 TCGA
XhoII 100 AGATCC [GA]^GATC[CT]
226 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI SspBI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI