##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BclI
BglI BlnI BsaAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT14I
EcoT22I FbaI FseI FspI HaeII
KasI KpnI KspI MluI MluNI
MscI NarI NcoI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 920 GTAGAC GT^[AC][GT]AC
AccIII 1161 TCCGGA T^CCGGA
AcyI 870 GGCGTC G[GA]^CG[CT]C
AflII 475 CTTAAG C^TTAAG
ApaI 83 GGGCCC GGGCC^C
AvaII 892 GGACC G^G[AT]CC
BanI 431 GGCACC G^G[CT][GA]CC
BbvCI 1393 CCTCAGC CCTCAGC(-5/-2)
BciVI 1004 GTATCC GTATCC(6/5)
BfrI 475 CTTAAG C^TTAAG
BglII 1471 AGATCT A^GATCT
BsaHI 870 GGCGTC G[GA]^CG[CT]C
BsaWI 1161 TCCGGA [AT]^CCGG[AT]
BseAI 1161 TCCGGA T^CCGGA
BsmBI 153 GAGACG CGTCTC(1/5)
BsmFI 445 GGGAC GGGAC(10/14)
BspEI 1161 TCCGGA T^CCGGA
BspHI 1635 TCATGA T^CATGA
BspMI 924 ACCTGC ACCTGC(4/8)
BsrFI 16 GCCGGC [GA]^CCGG[CT]
BstXI 1539 CCAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
DraI 643 TTTAAA TTT^AAA
DraII 83 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 1117 CCACGG C^C[GA][CT]GG
EcoO109I 83 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1557 GAATTC G^AATTC
Hin1I 870 GGCGTC G[GA]^CG[CT]C
HindIII 943 AAGCTT A^AGCTT
HpaI 500 GTTAAC GTT^AAC
MfeI 849 CAATTG C^AATTG
MroI 1161 TCCGGA T^CCGGA
MunI 849 CAATTG C^AATTG
NaeI 16 GCCGGC GCC^GGC
NdeI 2153 CATATG CA^TATG
NgoMIV 16 GCCGGC G^CCGGC
PleI 1352 GACTC GAGTC(4/5)
RcaI 1635 TCATGA T^CATGA
SapI 1852 GCTCTTC GCTCTTC(1/4)
ScaI 1250 AGTACT AGT^ACT
SmaI 80 CCCGGG CCC^GGG
StuI 1303 AGGCCT AGG^CCT
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 68 CGCG CG^CG
415 CGCG
AlwNI 655 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1233 CAGATGCTG
AspHI 266 GAGCTC G[AT]GC[AT]^C
1688 GAGCTC
AvaI 80 CCCGGG C^[CT]CG[GA]G
253 CCCGAG
BlpI 272 GCTGAGC GC^T[ACGT]AGC
577 GCTTAGC
Bpu1102I 272 GCTGAGC GC^T[ACGT]AGC
577 GCTTAGC
BsaBI 1016 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1022 GATCTCTATC
BsaI 1421 GAGACC GGTCTC(1/5)
1598 GAGACC
BsiHKAI 266 GAGCTC G[AT]GC[AT]^C
1688 GAGCTC
BsoBI 80 CCCGGG C^[CT]CG[GA]G
253 CCCGAG
BsrDI 454 CATTGC GCAATG(2/0)
2044 CATTGC
BstUI 68 CGCG CG^CG
415 CGCG
BstYI 1471 AGATCT [GA]^GATC[CT]
1627 GGATCT
CelII 272 GCTGAGC GC^T[ACGT]AGC
577 GCTTAGC
CfoI 49 GCGC GCG^C
69 GCGC
EcoRV 986 GATATC GAT^ATC
1310 GATATC
FauI 170 CCCGC CCCGC(4/6)
27 GCGGG
HhaI 49 GCGC GCG^C
69 GCGC
HinP1I 49 GCGC G^CGC
69 GCGC
HincII 500 GTTAAC GT[CT]^[GA]AC
827 GTCAAC
HindII 500 GTTAAC GT[CT]^[GA]AC
827 GTCAAC
MaeII 772 ACGT A^CGT
2103 ACGT
MamI 1016 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1022 GATCTCTATC
MflI 1471 AGATCT [GA]^GATC[CT]
1627 GGATCT
MvnI 68 CGCG CG^CG
415 CGCG
PvuII 981 CAGCTG CAG^CTG
1544 CAGCTG
SacI 266 GAGCTC GAGCT^C
1688 GAGCTC
SfcI 591 CTACAG C^T[GA][CT]AG
1487 CTACAG
XbaI 2073 TCTAGA T^CTAGA
2129 TCTAGA
XhoII 1471 AGATCT [GA]^GATC[CT]
1627 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NheI NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI