##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI Alw44I Aor51HI
ApaLI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbeI BbrPI
BbsI BcgI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII EagI EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1453 GTCTAC GT^[AC][GT]AC
AcsI 51 AAATTT [GA]^AATT[CT]
AflIII 698 ACGTGT A^C[GA][CT]GT
AhdI 64 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 51 AAATTT [GA]^AATT[CT]
AscI 11 GGCGCGCC GG^CGCGCC
AspEI 64 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1577 TGCGCA TGC^GCA
BbvCI 993 CCTCAGC CCTCAGC(-5/-2)
BlpI 1107 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1107 GCTGAGC GC^T[ACGT]AGC
BsaI 435 GAGACC GGTCTC(1/5)
BsmI 175 GAATGC GAATGC(1/-1)
BsrFI 392 GCCGGC [GA]^CCGG[CT]
BssHII 12 GCGCGC G^CGCGC
BstAPI 594 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1233 GGTCACC G^GT[ACGT]ACC
BstPI 1233 GGTCACC G^GT[ACGT]ACC
CelII 1107 GCTGAGC GC^T[ACGT]AGC
Cfr10I 392 GCCGGC [GA]^CCGG[CT]
DrdI 643 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1124 CCGTGG C^C[GA][CT]GG
Eam1105I 64 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 573 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1233 GGTCACC G^GT[ACGT]ACC
EcoT14I 204 CCTTGG C^C[AT][AT]GG
FspI 1577 TGCGCA TGC^GCA
HincII 79 GTCAAC GT[CT]^[GA]AC
HindII 79 GTCAAC GT[CT]^[GA]AC
MseI 1079 TTAA T^TAA
NaeI 392 GCCGGC GCC^GGC
NgoMIV 392 GCCGGC G^CCGGC
PflMI 897 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1500 AGGTCCT [GA]G^G[AT]CC[CT]
StuI 1641 AGGCCT AGG^CCT
StyI 204 CCTTGG C^C[AT][AT]GG
TfiI 1018 GATTC G^A[AT]TC
Tru9I 1079 TTAA T^TAA
Van91I 897 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1220 CCACGCTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 898 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1382 CAGCCCCTG
AocI 511 CCTGAGG CC^T[ACGT]AGG
1504 CCTGAGG
AspHI 592 GAGCAC G[AT]GC[AT]^C
1743 GAGCAC
BalI 248 TGGCCA TGG^CCA
1205 TGGCCA
BciVI 36 GTATCC GTATCC(6/5)
110 GTATCC
BfaI 373 CTAG C^TAG
1396 CTAG
BsgI 121 GTGCAG GTGCAG(16/14)
948 GTGCAG
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
1743 GAGCAC
BsmBI 963 CGTCTC CGTCTC(1/5)
749 GAGACG
BspMI 599 ACCTGC ACCTGC(4/8)
1574 ACCTGC
BstYI 1094 AGATCC [GA]^GATC[CT]
1340 AGATCC
Bsu36I 511 CCTGAGG CC^T[ACGT]AGG
1504 CCTGAGG
EaeI 248 TGGCCA [CT]^GGCC[GA]
1205 TGGCCA
Eco81I 511 CCTGAGG CC^T[ACGT]AGG
1504 CCTGAGG
HaeII 952 AGCGCC [GA]GCGC^[CT]
1111 AGCGCC
MaeI 373 CTAG C^TAG
1396 CTAG
MflI 1094 AGATCC [GA]^GATC[CT]
1340 AGATCC
MluNI 248 TGGCCA TGG^CCA
1205 TGGCCA
MscI 248 TGGCCA TGG^CCA
1205 TGGCCA
NspI 1193 GCATGT [GA]CATG^[CT]
1449 GCATGT
PleI 1661 GACTC GAGTC(4/5)
1265 GAGTC
SmaI 326 CCCGGG CCC^GGG
968 CCCGGG
Tsp509I 52 AATT ^AATT
1159 AATT
XhoII 1094 AGATCC [GA]^GATC[CT]
1340 AGATCC
XmaCI 326 CCCGGG C^CCGGG
968 CCCGGG
XmaI 326 CCCGGG C^CCGGG
968 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI