################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I ClaI DraIII DrdI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI Tsp509I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 1718 GACGCC G[GA]^CG[CT]C AfaI 1051 GTAC GT^AC AhdI 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 686 GAGCAC G[AT]GC[AT]^C AspI 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 557 GGCACC G^G[CT][GA]CC BbsI 1356 GTCTTC GAAGAC(2/6) BbvCI 1783 CCTCAGC CCTCAGC(-5/-2) BglI 1692 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 362 GCTCAGC GC^T[ACGT]AGC Bpu1102I 362 GCTCAGC GC^T[ACGT]AGC BpuAI 1356 GTCTTC GAAGAC(2/6) BsaHI 1718 GACGCC G[GA]^CG[CT]C BsiEI 1729 CGGCCG CG[GA][CT]^CG BsiHKAI 686 GAGCAC G[AT]GC[AT]^C BsrDI 265 CATTGC GCAATG(2/0) BstYI 1767 AGATCC [GA]^GATC[CT] CelII 362 GCTCAGC GC^T[ACGT]AGC DraI 2240 TTTAAA TTT^AAA EagI 1729 CGGCCG C^GGCCG Eam1105I 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1729 CGGCCG C^GGCCG Eco52I 1729 CGGCCG C^GGCCG HaeII 829 AGCGCC [GA]GCGC^[CT] HgaI 1718 GACGC GACGC(5/10) Hin1I 1718 GACGCC G[GA]^CG[CT]C HindIII 59 AAGCTT A^AGCTT MaeII 1814 ACGT A^CGT MflI 1767 AGATCC [GA]^GATC[CT] NaeI 770 GCCGGC GCC^GGC NgoMIV 770 GCCGGC G^CCGGC PflFI 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 134 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 1051 GTAC GT^AC SexAI 2068 ACCTGGT A^CC[AT]GGT SmaI 730 CCCGGG CCC^GGG SmlI 696 CTTGAG C^T[CT][GA]AG SpeI 2227 ACTAGT A^CTAGT TaqI 85 TCGA T^CGA Tth111I 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 85 TCGA T^CGA Van91I 134 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 200 TCTAGA T^CTAGA XcmI 1587 CCATCTATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1767 AGATCC [GA]^GATC[CT] XmaCI 730 CCCGGG C^CCGGG XmaI 730 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaI 553 CTCGGG C^[CT]CG[GA]G 730 CCCGGG BalI 448 TGGCCA TGG^CCA 1584 TGGCCA BsoBI 553 CTCGGG C^[CT]CG[GA]G 730 CCCGGG BspMI 1685 ACCTGC ACCTGC(4/8) 485 GCAGGT BstAPI 1664 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1873 GCAGCCCCTGC CfoI 830 GCGC GCG^C 1708 GCGC CpoI 107 CGGTCCG CG^G[AT]CCG 1724 CGGACCG HhaI 830 GCGC GCG^C 1708 GCGC HinP1I 830 GCGC G^CGC 1708 GCGC MluNI 448 TGGCCA TGG^CCA 1584 TGGCCA MscI 448 TGGCCA TGG^CCA 1584 TGGCCA NcoI 65 CCATGG C^CATGG 2134 CCATGG RsrII 107 CGGTCCG CG^G[AT]CCG 1724 CGGACCG StuI 784 AGGCCT AGG^CCT 1853 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AluI AlwI AlwNI ApaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I TspRI