##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I ClaI DraIII DrdI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tsp509I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 1718 GACGCC G[GA]^CG[CT]C
AfaI 1051 GTAC GT^AC
AhdI 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 686 GAGCAC G[AT]GC[AT]^C
AspI 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 557 GGCACC G^G[CT][GA]CC
BbsI 1356 GTCTTC GAAGAC(2/6)
BbvCI 1783 CCTCAGC CCTCAGC(-5/-2)
BglI 1692 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 362 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 362 GCTCAGC GC^T[ACGT]AGC
BpuAI 1356 GTCTTC GAAGAC(2/6)
BsaHI 1718 GACGCC G[GA]^CG[CT]C
BsiEI 1729 CGGCCG CG[GA][CT]^CG
BsiHKAI 686 GAGCAC G[AT]GC[AT]^C
BsrDI 265 CATTGC GCAATG(2/0)
BstYI 1767 AGATCC [GA]^GATC[CT]
CelII 362 GCTCAGC GC^T[ACGT]AGC
DraI 2240 TTTAAA TTT^AAA
EagI 1729 CGGCCG C^GGCCG
Eam1105I 1179 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1729 CGGCCG C^GGCCG
Eco52I 1729 CGGCCG C^GGCCG
HaeII 829 AGCGCC [GA]GCGC^[CT]
HgaI 1718 GACGC GACGC(5/10)
Hin1I 1718 GACGCC G[GA]^CG[CT]C
HindIII 59 AAGCTT A^AGCTT
MaeII 1814 ACGT A^CGT
MflI 1767 AGATCC [GA]^GATC[CT]
NaeI 770 GCCGGC GCC^GGC
NgoMIV 770 GCCGGC G^CCGGC
PflFI 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 134 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 1051 GTAC GT^AC
SexAI 2068 ACCTGGT A^CC[AT]GGT
SmaI 730 CCCGGG CCC^GGG
SmlI 696 CTTGAG C^T[CT][GA]AG
SpeI 2227 ACTAGT A^CTAGT
TaqI 85 TCGA T^CGA
Tth111I 1810 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 85 TCGA T^CGA
Van91I 134 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 200 TCTAGA T^CTAGA
XcmI 1587 CCATCTATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1767 AGATCC [GA]^GATC[CT]
XmaCI 730 CCCGGG C^CCGGG
XmaI 730 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaI 553 CTCGGG C^[CT]CG[GA]G
730 CCCGGG
BalI 448 TGGCCA TGG^CCA
1584 TGGCCA
BsoBI 553 CTCGGG C^[CT]CG[GA]G
730 CCCGGG
BspMI 1685 ACCTGC ACCTGC(4/8)
485 GCAGGT
BstAPI 1664 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1873 GCAGCCCCTGC
CfoI 830 GCGC GCG^C
1708 GCGC
CpoI 107 CGGTCCG CG^G[AT]CCG
1724 CGGACCG
HhaI 830 GCGC GCG^C
1708 GCGC
HinP1I 830 GCGC G^CGC
1708 GCGC
MluNI 448 TGGCCA TGG^CCA
1584 TGGCCA
MscI 448 TGGCCA TGG^CCA
1584 TGGCCA
NcoI 65 CCATGG C^CATGG
2134 CCATGG
RsrII 107 CGGTCCG CG^G[AT]CCG
1724 CGGACCG
StuI 784 AGGCCT AGG^CCT
1853 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I TspRI