##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI ApaI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BfrI BlnI BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRV FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MluI MluNI MroI
MscI MslI NarI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 1329 GTAC GT^AC
Alw44I 1506 GTGCAC G^TGCAC
Aor51HI 259 AGCGCT AGC^GCT
ApaLI 1506 GTGCAC G^TGCAC
AseI 1380 ATTAAT AT^TAAT
AsnI 1380 ATTAAT AT^TAAT
BanI 366 GGTGCC G^G[CT][GA]CC
BciVI 1635 GTATCC GTATCC(6/5)
BglII 1068 AGATCT A^GATCT
BlpI 822 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 822 GCTGAGC GC^T[ACGT]AGC
BsaBI 327 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 336 CTCCTC GAGGAG(10/8)
BsgI 1072 CTGCAC GTGCAG(16/14)
BsmI 767 GAATGC GAATGC(1/-1)
BssHII 11 GCGCGC G^CGCGC
BstXI 715 CCATCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 822 GCTGAGC GC^T[ACGT]AGC
DraII 957 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 415 CCATGG C^C[GA][CT]GG
Eco47III 259 AGCGCT AGC^GCT
EcoO109I 957 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1531 ATGCAT ATGCA^T
FseI 80 GGCCGGCC GGCCGG^CC
MamI 327 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1579 CAATTG C^AATTG
MunI 1579 CAATTG C^AATTG
NcoI 415 CCATGG C^CATGG
NheI 156 GCTAGC G^CTAGC
NsiI 1531 ATGCAT ATGCA^T
NspI 1340 GCATGC [GA]CATG^[CT]
PleI 858 GACTC GAGTC(4/5)
PpuMI 957 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1380 ATTAAT AT^TAAT
PvuII 222 CAGCTG CAG^CTG
RsaI 1329 GTAC GT^AC
SacI 1150 GAGCTC GAGCT^C
SphI 1340 GCATGC GCATG^C
Tsp45I 294 GTGAC ^GT[GC]AC
XcmI 817 CCATAGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1352 GAATTC [GA]^AATT[CT]
1720 GAATTC
AlwNI 222 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
876 CAGTTGCTG
ApoI 1352 GAATTC [GA]^AATT[CT]
1720 GAATTC
AspHI 1150 GAGCTC G[AT]GC[AT]^C
1506 GTGCAC
AvaII 212 GGACC G^G[AT]CC
958 GGTCC
BanII 1140 GAGCCC G[GA]GC[CT]^C
1150 GAGCTC
BclI 529 TGATCA T^GATCA
868 TGATCA
BglI 246 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1262 GCCAACCAGGC
BpmI 383 CTGGAG CTGGAG(16/14)
483 CTGGAG
BsiHKAI 1150 GAGCTC G[AT]GC[AT]^C
1506 GTGCAC
BsmFI 211 GGGAC GGGAC(10/14)
959 GTCCC
BsrBI 40 GAGCGG CCGCTC(-3/-3)
134 GAGCGG
BsrI 94 CCAGT ACTGG(1/-1)
551 CCAGT
BstAPI 221 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
875 GCAGTTGCTGC
EagI 79 CGGCCG C^GGCCG
83 CGGCCG
EclXI 79 CGGCCG C^GGCCG
83 CGGCCG
Eco52I 79 CGGCCG C^GGCCG
83 CGGCCG
EcoRI 1352 GAATTC G^AATTC
1720 GAATTC
EcoT14I 415 CCATGG C^C[AT][AT]GG
1478 CCTTGG
FbaI 529 TGATCA T^GATCA
868 TGATCA
HindIII 454 AAGCTT A^AGCTT
649 AAGCTT
MaeII 1459 ACGT A^CGT
1504 ACGT
NaeI 81 GCCGGC GCC^GGC
85 GCCGGC
NgoMIV 81 GCCGGC G^CCGGC
85 GCCGGC
StyI 415 CCATGG C^C[AT][AT]GG
1478 CCTTGG
TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^
638 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BfaI BmyI BsaJI BsiEI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrFI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I
XhoII