##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI BamHI BbeI BbvCI BcgI
BciVI BclI BglII BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MslI MunI NarI NdeI
NotI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 17 GTAGAC GT^[AC][GT]AC
AccIII 1227 TCCGGA T^CCGGA
AcsI 1641 AAATTT [GA]^AATT[CT]
AfaI 242 GTAC GT^AC
AflII 1307 CTTAAG C^TTAAG
AhdI 129 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1641 AAATTT [GA]^AATT[CT]
AspEI 129 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1478 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1020 TGCGCA TGC^GCA
AvrII 799 CCTAGG C^CTAGG
BbrPI 575 CACGTG CAC^GTG
BbsI 1121 GAAGAC GAAGAC(2/6)
BfrI 1307 CTTAAG C^TTAAG
BlnI 799 CCTAGG C^CTAGG
BlpI 42 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTAAGC GC^T[ACGT]AGC
BpuAI 1121 GAAGAC GAAGAC(2/6)
BseAI 1227 TCCGGA T^CCGGA
BsmAI 50 GAGAC GTCTC(1/5)
BspEI 1227 TCCGGA T^CCGGA
BsrFI 617 GCCGGC [GA]^CCGG[CT]
BssHII 1393 GCGCGC G^CGCGC
BssSI 659 CTCGTG CACGAG(-5/-1)
BstXI 1197 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 42 GCTAAGC GC^T[ACGT]AGC
Cfr10I 617 GCCGGC [GA]^CCGG[CT]
Eam1105I 129 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1020 TGCGCA TGC^GCA
HindIII 1442 AAGCTT A^AGCTT
KspI 1000 CCGCGG CCGC^GG
MroI 1227 TCCGGA T^CCGGA
MseI 1308 TTAA T^TAA
NaeI 617 GCCGGC GCC^GGC
NgoMIV 617 GCCGGC G^CCGGC
NheI 869 GCTAGC G^CTAGC
NruI 345 TCGCGA TCG^CGA
NspI 1455 GCATGT [GA]CATG^[CT]
PaeR7I 498 CTCGAG C^TCGAG
PflFI 1478 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 239 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 575 CACGTG CAC^GTG
PmlI 575 CACGTG CAC^GTG
RsaI 242 GTAC GT^AC
SacII 1000 CCGCGG CCGC^GG
SapI 770 GAAGAGC GCTCTTC(1/4)
SfiI 1059 GGCCCCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 499 TCGA T^CGA
Tru9I 1308 TTAA T^TAA
Tth111I 1478 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 499 TCGA T^CGA
Van91I 239 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 930 CCAGCCTGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 498 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BalI 948 TGGCCA TGG^CCA
1535 TGGCCA
BfaI 800 CTAG C^TAG
870 CTAG
BsaAI 575 CACGTG [CT]AC^GT[GA]
1388 TACGTG
BsaWI 1227 TCCGGA [AT]^CCGG[AT]
1467 ACCGGA
BsgI 750 CTGCAC GTGCAG(16/14)
120 GTGCAG
BsmI 1023 GCATTC GAATGC(1/-1)
1161 GCATTC
BspMI 310 GCAGGT ACCTGC(4/8)
364 GCAGGT
BsrBI 597 CCGCTC CCGCTC(-3/-3)
1546 CCGCTC
BsrDI 957 CATTGC GCAATG(2/0)
1251 GCAATG
BsrI 239 CCAGT ACTGG(1/-1)
1630 CCAGT
BstAPI 392 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
893 GCAGGAGGTGC
BstYI 1230 GGATCT [GA]^GATC[CT]
1243 GGATCT
EarI 770 GAAGAG CTCTTC(1/4)
830 GAAGAG
EcoNI 437 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1519 CCTGAGATAGG
HaeII 149 GGCGCT [GA]GCGC^[CT]
1563 GGCGCT
HgaI 556 GCGTC GACGC(5/10)
1045 GCGTC
Ksp632I 770 GAAGAG CTCTTC(1/4)
830 GAAGAG
MaeI 800 CTAG C^TAG
870 CTAG
MaeIII 477 GTCAC ^GT[ACGT]AC
1326 GTGAC
MflI 1230 GGATCT [GA]^GATC[CT]
1243 GGATCT
MluNI 948 TGGCCA TGG^CCA
1535 TGGCCA
MscI 948 TGGCCA TGG^CCA
1535 TGGCCA
NcoI 420 CCATGG C^CATGG
744 CCATGG
StuI 802 AGGCCT AGG^CCT
1084 AGGCCT
Tsp45I 477 GTCAC ^GT[GC]AC
1326 GTGAC
XhoII 1230 GGATCT [GA]^GATC[CT]
1243 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI TseI
Tsp509I TspRI XmaCI XmaI