##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BbeI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BsgI BsiWI BspDI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
Eam1105I EarI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccI 164 GTCGAC GT^[AC][GT]AC
AccII 373 CGCG CG^CG
AccIII 1649 TCCGGA T^CCGGA
AocI 1780 CCTTAGG CC^T[ACGT]AGG
AseI 761 ATTAAT AT^TAAT
AsnI 761 ATTAAT AT^TAAT
Asp700I 356 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 260 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1178 CCCGAG C^[CT]CG[GA]G
BalI 465 TGGCCA TGG^CCA
BbrPI 1255 CACGTG CAC^GTG
BbvCI 1581 CCTCAGC CCTCAGC(-5/-2)
BglI 651 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1255 CACGTG [CT]AC^GT[GA]
BsaWI 1649 TCCGGA [AT]^CCGG[AT]
BseAI 1649 TCCGGA T^CCGGA
BsiEI 439 CGGCCG CG[GA][CT]^CG
BsmBI 897 GAGACG CGTCTC(1/5)
BsmI 360 GCATTC GAATGC(1/-1)
BsoBI 1178 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1363 TGTACA T^GTACA
BspEI 1649 TCCGGA T^CCGGA
BspMI 1833 ACCTGC ACCTGC(4/8)
BsrDI 609 GCAATG GCAATG(2/0)
BsrGI 1363 TGTACA T^GTACA
BstAPI 1159 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 373 CGCG CG^CG
BstXI 1321 CCATGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1780 CCTTAGG CC^T[ACGT]AGG
CfoI 372 GCGC GCG^C
DraIII 1309 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1420 GACCTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1508 CCGTGG C^C[GA][CT]GG
EagI 439 CGGCCG C^GGCCG
EclXI 439 CGGCCG C^GGCCG
Eco52I 439 CGGCCG C^GGCCG
Eco81I 1780 CCTTAGG CC^T[ACGT]AGG
EcoNI 535 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 381 CCAAGG C^C[AT][AT]GG
HhaI 372 GCGC GCG^C
HinP1I 372 GCGC G^CGC
HincII 164 GTCGAC GT[CT]^[GA]AC
HindII 164 GTCGAC GT[CT]^[GA]AC
MluNI 465 TGGCCA TGG^CCA
MroI 1649 TCCGGA T^CCGGA
MscI 465 TGGCCA TGG^CCA
MslI 922 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 373 CGCG CG^CG
PflFI 260 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1568 GAGTC GAGTC(4/5)
PmaCI 1255 CACGTG CAC^GTG
PmlI 1255 CACGTG CAC^GTG
PpuMI 1830 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 761 ATTAAT AT^TAAT
SalI 164 GTCGAC G^TCGAC
ScaI 1735 AGTACT AGT^ACT
SmlI 1573 CTTGAG C^T[CT][GA]AG
SspBI 1363 TGTACA T^GTACA
SspI 764 AATATT AAT^ATT
StuI 102 AGGCCT AGG^CCT
StyI 381 CCAAGG C^C[AT][AT]GG
TfiI 1333 GAATC G^A[AT]TC
Tth111I 260 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 681 CCATCTTCCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 356 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 161 GACGTC GACGT^C
620 GACGTC
AcsI 590 AAATTC [GA]^AATT[CT]
1358 AAATTT
AcyI 161 GACGTC G[GA]^CG[CT]C
620 GACGTC
ApoI 590 AAATTC [GA]^AATT[CT]
1358 AAATTT
BamHI 942 GGATCC G^GATCC
1728 GGATCC
BanI 473 GGCACC G^G[CT][GA]CC
495 GGTGCC
BglII 483 AGATCT A^GATCT
632 AGATCT
BsaHI 161 GACGTC G[GA]^CG[CT]C
620 GACGTC
BseRI 859 CTCCTC GAGGAG(10/8)
524 GAGGAG
BsmFI 1395 GGGAC GGGAC(10/14)
1830 GGGAC
BsrFI 90 ACCGGC [GA]^CCGG[CT]
437 ACCGGC
BstEII 152 GGTTACC G^GT[ACGT]ACC
301 GGTCACC
BstPI 152 GGTTACC G^GT[ACGT]ACC
301 GGTCACC
Cfr10I 90 ACCGGC [GA]^CCGG[CT]
437 ACCGGC
EaeI 439 CGGCCG [CT]^GGCC[GA]
465 TGGCCA
EcoO65I 152 GGTTACC G^GT[ACGT]ACC
301 GGTCACC
FauI 1521 CCCGC CCCGC(4/6)
2 GCGGG
HgaI 899 GACGC GACGC(5/10)
1820 GACGC
Hin1I 161 GACGTC G[GA]^CG[CT]C
620 GACGTC
HinfI 1333 GAATC G^A[ACGT]TC
1568 GAGTC
MseI 762 TTAA T^TAA
1800 TTAA
PvuII 417 CAGCTG CAG^CTG
1769 CAGCTG
SexAI 120 ACCTGGT A^CC[AT]GGT
990 ACCTGGT
TaqI 33 TCGA T^CGA
165 TCGA
Tru9I 762 TTAA T^TAA
1800 TTAA
TthHB8I 33 TCGA T^CGA
165 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I Tsp509I
TspRI XhoII