##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Alw44I ApaI ApaLI AscI AspEI
AviII AvrII BamHI BcgI BciVI
BfrI BlnI BseAI BsiEI BsiWI
BspDI BspEI BspMI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FseI
FspI HpaI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflMI PpuMI PshAI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 575 GGTACC G^GTACC
AccI 828 GTAGAC GT^[AC][GT]AC
AgeI 1494 ACCGGT A^CCGGT
AocI 1716 CCTGAGG CC^T[ACGT]AGG
AseI 2086 ATTAAT AT^TAAT
AsnI 2086 ATTAAT AT^TAAT
Asp718I 575 GGTACC G^GTACC
AspHI 76 GAGCTC G[AT]GC[AT]^C
AspI 644 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1469 CCCGAG C^[CT]CG[GA]G
BbeI 127 GGCGCC GGCGC^C
BbrPI 2253 CACGTG CAC^GTG
BclI 846 TGATCA T^GATCA
BglI 1053 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1168 AGATCT A^GATCT
BlpI 1397 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1397 GCTGAGC GC^T[ACGT]AGC
BsaAI 2253 CACGTG [CT]AC^GT[GA]
BsaBI 2196 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1494 ACCGGT [AT]^CCGG[AT]
BsiHKAI 76 GAGCTC G[AT]GC[AT]^C
BsmBI 86 GAGACG CGTCTC(1/5)
BsmI 694 GCATTC GAATGC(1/-1)
BsoBI 1469 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2771 TGTACA T^GTACA
BspLU11I 2542 ACATGT A^CATGT
BsrGI 2771 TGTACA T^GTACA
BssSI 356 CACGAG CACGAG(-5/-1)
BstXI 1970 CCAGTAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1716 CCTGAGG CC^T[ACGT]AGG
CelII 1397 GCTGAGC GC^T[ACGT]AGC
Eco81I 1716 CCTGAGG CC^T[ACGT]AGG
FbaI 846 TGATCA T^GATCA
KasI 127 GGCGCC G^GCGCC
KpnI 575 GGTACC GGTAC^C
KspI 131 CCGCGG CCGC^GG
MamI 2196 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1356 CAATTG C^AATTG
MunI 1356 CAATTG C^AATTG
NarI 127 GGCGCC GG^CGCC
NcoI 1023 CCATGG C^CATGG
PacI 2087 TTAATTAA TTAAT^TAA
PflFI 644 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1494 ACCGGT A^CCGGT
PmaCI 2253 CACGTG CAC^GTG
PmeI 1542 GTTTAAAC GTTT^AAAC
PmlI 2253 CACGTG CAC^GTG
PshBI 2086 ATTAAT AT^TAAT
PstI 889 CTGCAG CTGCA^G
SacI 76 GAGCTC GAGCT^C
SacII 131 CCGCGG CCGC^GG
SfoI 127 GGCGCC GGC^GCC
SmlI 215 CTTGAG C^T[CT][GA]AG
SphI 1981 GCATGC GCATG^C
SspBI 2771 TGTACA T^GTACA
Tth111I 644 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 623 AAATTT [GA]^AATT[CT]
2444 AAATTT
AflIII 1010 ACACGT A^C[GA][CT]GT
2542 ACATGT
ApoI 623 AAATTT [GA]^AATT[CT]
2444 AAATTT
AvaII 162 GGACC G^G[AT]CC
1492 GGACC
BalI 618 TGGCCA TGG^CCA
1825 TGGCCA
BanII 76 GAGCTC G[GA]GC[CT]^C
1400 GAGCCC
BbsI 1337 GAAGAC GAAGAC(2/6)
1853 GTCTTC
BcnI 92 CCGGG CC^[GC]GG
870 CCGGG
BpmI 1817 CTCCAG CTGGAG(16/14)
438 CTGGAG
BpuAI 1337 GAAGAC GAAGAC(2/6)
1853 GTCTTC
BsaI 1458 GAGACC GGTCTC(1/5)
2715 GAGACC
BspHI 745 TCATGA T^CATGA
2264 TCATGA
BsrBI 111 GAGCGG CCGCTC(-3/-3)
122 GAGCGG
BsrFI 59 GCCGGT [GA]^CCGG[CT]
1494 ACCGGT
Cfr10I 59 GCCGGT [GA]^CCGG[CT]
1494 ACCGGT
EarI 1093 GAAGAG CTCTTC(1/4)
1418 GAAGAG
EcoT14I 1023 CCATGG C^C[AT][AT]GG
2470 CCTTGG
EcoT22I 1884 ATGCAT ATGCA^T
2656 ATGCAT
HincII 1749 GTTGAC GT[CT]^[GA]AC
2181 GTTGAC
HindII 1749 GTTGAC GT[CT]^[GA]AC
2181 GTTGAC
HindIII 2008 AAGCTT A^AGCTT
2047 AAGCTT
HphI 1497 GGTGA GGTGA(8/7)
2399 GGTGA
Ksp632I 1093 GAAGAG CTCTTC(1/4)
1418 GAAGAG
MluNI 618 TGGCCA TGG^CCA
1825 TGGCCA
MscI 618 TGGCCA TGG^CCA
1825 TGGCCA
NciI 92 CCGGG CC^[GC]GG
870 CCGGG
NsiI 1884 ATGCAT ATGCA^T
2656 ATGCAT
PvuII 1434 CAGCTG CAG^CTG
1960 CAGCTG
RcaI 745 TCATGA T^CATGA
2264 TCATGA
SfcI 889 CTGCAG C^T[GA][CT]AG
2166 CTGTAG
StyI 1023 CCATGG C^C[AT][AT]GG
2470 CCTTGG
TaqI 930 TCGA T^CGA
2332 TCGA
TfiI 1139 GATTC G^A[AT]TC
2521 GATTC
TthHB8I 930 TCGA T^CGA
2332 TCGA
XcmI 315 CCAGTGGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1522 CCAGGACTTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI Asp700I BanI
BbvCI BbvI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI Eco47III EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI