##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BcgI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SfcI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 1134 GAATTT [GA]^AATT[CT]
AocI 1594 CCTGAGG CC^T[ACGT]AGG
ApaI 169 GGGCCC GGGCC^C
ApoI 1134 GAATTT [GA]^AATT[CT]
AvaI 626 CCCGAG C^[CT]CG[GA]G
BalI 217 TGGCCA TGG^CCA
BbeI 548 GGCGCC GGCGC^C
BbsI 1481 GAAGAC GAAGAC(2/6)
BciVI 776 GGATAC GTATCC(6/5)
BclI 64 TGATCA T^GATCA
BpuAI 1481 GAAGAC GAAGAC(2/6)
BsiEI 617 CGACCG CG[GA][CT]^CG
BsmBI 730 GAGACG CGTCTC(1/5)
BsoBI 626 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1070 TGTACA T^GTACA
BsrBI 1417 GAGCGG CCGCTC(-3/-3)
BsrGI 1070 TGTACA T^GTACA
BstXI 70 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1594 CCTGAGG CC^T[ACGT]AGG
DraIII 68 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1594 CCTGAGG CC^T[ACGT]AGG
FbaI 64 TGATCA T^GATCA
HphI 67 TCACC GGTGA(8/7)
KasI 548 GGCGCC G^GCGCC
MluNI 217 TGGCCA TGG^CCA
MscI 217 TGGCCA TGG^CCA
MslI 1080 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 548 GGCGCC GG^CGCC
NcoI 1249 CCATGG C^CATGG
NdeI 1080 CATATG CA^TATG
PflMI 840 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1545 GAGTC GAGTC(4/5)
ScaI 1373 AGTACT AGT^ACT
SexAI 69 ACCAGGT A^CC[AT]GGT
SfoI 548 GGCGCC GGC^GCC
SmlI 366 CTCAAG C^T[CT][GA]AG
SspBI 1070 TGTACA T^GTACA
Van91I 840 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 574 CCAGGAATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 376 GGCGTC G[GA]^CG[CT]C
548 GGCGCC
AlwNI 556 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1063 CAGAAGCTG
Asp700I 1046 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1254 GAATTGATTC
BpmI 648 CTCCAG CTGGAG(16/14)
390 CTGGAG
BsaHI 376 GGCGTC G[GA]^CG[CT]C
548 GGCGCC
BsaI 622 GAGACC GGTCTC(1/5)
1625 GAGACC
BsgI 314 CTGCAC GTGCAG(16/14)
405 GTGCAG
BspMI 123 ACCTGC ACCTGC(4/8)
202 ACCTGC
BsrDI 108 GCAATG GCAATG(2/0)
786 GCAATG
EcoT14I 845 CCTTGG C^C[AT][AT]GG
1249 CCATGG
HgaI 153 GACGC GACGC(5/10)
377 GCGTC
Hin1I 376 GGCGTC G[GA]^CG[CT]C
548 GGCGCC
MaeII 117 ACGT A^CGT
382 ACGT
MaeIII 234 GTGAC ^GT[ACGT]AC
379 GTCAC
MspA1I 165 CAGCGG C[AC]G^C[GT]G
270 CAGCGG
NspI 160 ACATGC [GA]CATG^[CT]
1441 ACATGC
SapI 7 GCTCTTC GCTCTTC(1/4)
1106 GCTCTTC
SfiI 218 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
512 GGCCATCAAGGCC
StyI 845 CCTTGG C^C[AT][AT]GG
1249 CCATGG
Tsp45I 234 GTGAC ^GT[GC]AC
379 GTCAC
XmnI 1046 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1254 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI