##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI AspI BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI Hin1I HindIII
HpaI KasI MaeII MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 561 CGCG CG^CG
AcsI 54 AAATTT [GA]^AATT[CT]
AhdI 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 547 AGCGCT AGC^GCT
ApoI 54 AAATTT [GA]^AATT[CT]
Asp700I 1759 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 747 GTGCTC G[AT]GC[AT]^C
AvaI 1300 CTCGAG C^[CT]CG[GA]G
AviII 1950 TGCGCA TGC^GCA
AvrII 1797 CCTAGG C^CTAGG
BbsI 506 GAAGAC GAAGAC(2/6)
BciVI 1966 GGATAC GTATCC(6/5)
BlnI 1797 CCTAGG C^CTAGG
BpuAI 506 GAAGAC GAAGAC(2/6)
BsaI 1751 GAGACC GGTCTC(1/5)
BsiEI 563 CGGCCG CG[GA][CT]^CG
BsiHKAI 747 GTGCTC G[AT]GC[AT]^C
BsoBI 1300 CTCGAG C^[CT]CG[GA]G
BspMI 494 GCAGGT ACCTGC(4/8)
BsrBI 875 CCGCTC CCGCTC(-3/-3)
BstAPI 105 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 561 CGCG CG^CG
BstXI 594 CCACTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 141 TTTAAA TTT^AAA
EagI 563 CGGCCG C^GGCCG
Eam1105I 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 563 CGGCCG C^GGCCG
Eco47III 547 AGCGCT AGC^GCT
Eco52I 563 CGGCCG C^GGCCG
FspI 1950 TGCGCA TGC^GCA
KspI 560 CCGCGG CCGC^GG
MvnI 561 CGCG CG^CG
NaeI 514 GCCGGC GCC^GGC
NcoI 2073 CCATGG C^CATGG
NgoMIV 514 GCCGGC G^CCGGC
PaeR7I 1300 CTCGAG C^TCGAG
PshAI 1298 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 669 CAGCTG CAG^CTG
SacII 560 CCGCGG CCGC^GG
SfiI 1992 GGCCAGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1300 CTCGAG C^T[CT][GA]AG
SspI 616 AATATT AAT^ATT
StuI 1850 AGGCCT AGG^CCT
TaqI 1301 TCGA T^CGA
TthHB8I 1301 TCGA T^CGA
XhoI 1300 CTCGAG C^TCGAG
XmnI 1759 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 355 TCCGGA T^CCGGA
586 TCCGGA
ApaI 27 GGGCCC GGGCC^C
820 GGGCCC
BalI 131 TGGCCA TGG^CCA
1991 TGGCCA
BglI 1704 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1993 GCCAGTCTGGC
BlpI 543 GCTGAGC GC^T[ACGT]AGC
1113 GCTCAGC
Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC
1113 GCTCAGC
BsaWI 355 TCCGGA [AT]^CCGG[AT]
586 TCCGGA
BseAI 355 TCCGGA T^CCGGA
586 TCCGGA
BspEI 355 TCCGGA T^CCGGA
586 TCCGGA
BspHI 1055 TCATGA T^CATGA
1492 TCATGA
BsrDI 742 GCAATG GCAATG(2/0)
1659 GCAATG
BsrFI 514 GCCGGC [GA]^CCGG[CT]
1234 ACCGGC
BstYI 352 AGATCC [GA]^GATC[CT]
1922 GGATCT
CelII 543 GCTGAGC GC^T[ACGT]AGC
1113 GCTCAGC
Cfr10I 514 GCCGGC [GA]^CCGG[CT]
1234 ACCGGC
EcoNI 880 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2023 CCTGGGGTAGG
HaeII 547 AGCGCT [GA]GCGC^[CT]
1972 GGCGCT
HincII 215 GTTGAC GT[CT]^[GA]AC
1287 GTCAAC
HindII 215 GTTGAC GT[CT]^[GA]AC
1287 GTCAAC
MflI 352 AGATCC [GA]^GATC[CT]
1922 GGATCT
MluNI 131 TGGCCA TGG^CCA
1991 TGGCCA
MroI 355 TCCGGA T^CCGGA
586 TCCGGA
MscI 131 TGGCCA TGG^CCA
1991 TGGCCA
RcaI 1055 TCATGA T^CATGA
1492 TCATGA
SapI 1263 GAAGAGC GCTCTTC(1/4)
749 GCTCTTC
TfiI 590 GATTC G^A[AT]TC
1024 GATTC
XcmI 994 CCACACTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1716 CCAGCCCCAGACTGG
XhoII 352 AGATCC [GA]^GATC[CT]
1922 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI Asp718I AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I