################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI AspI BamHI BbeI BbrPI BcgI BclI BfrI BglII BsaAI BsaBI BsaHI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HindIII HpaI KasI MaeII MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 561 CGCG CG^CG AcsI 54 AAATTT [GA]^AATT[CT] AhdI 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 547 AGCGCT AGC^GCT ApoI 54 AAATTT [GA]^AATT[CT] Asp700I 1759 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 747 GTGCTC G[AT]GC[AT]^C AvaI 1300 CTCGAG C^[CT]CG[GA]G AviII 1950 TGCGCA TGC^GCA AvrII 1797 CCTAGG C^CTAGG BbsI 506 GAAGAC GAAGAC(2/6) BciVI 1966 GGATAC GTATCC(6/5) BlnI 1797 CCTAGG C^CTAGG BpuAI 506 GAAGAC GAAGAC(2/6) BsaI 1751 GAGACC GGTCTC(1/5) BsiEI 563 CGGCCG CG[GA][CT]^CG BsiHKAI 747 GTGCTC G[AT]GC[AT]^C BsoBI 1300 CTCGAG C^[CT]CG[GA]G BspMI 494 GCAGGT ACCTGC(4/8) BsrBI 875 CCGCTC CCGCTC(-3/-3) BstAPI 105 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 561 CGCG CG^CG BstXI 594 CCACTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 141 TTTAAA TTT^AAA EagI 563 CGGCCG C^GGCCG Eam1105I 1400 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 563 CGGCCG C^GGCCG Eco47III 547 AGCGCT AGC^GCT Eco52I 563 CGGCCG C^GGCCG FspI 1950 TGCGCA TGC^GCA KspI 560 CCGCGG CCGC^GG MvnI 561 CGCG CG^CG NaeI 514 GCCGGC GCC^GGC NcoI 2073 CCATGG C^CATGG NgoMIV 514 GCCGGC G^CCGGC PaeR7I 1300 CTCGAG C^TCGAG PshAI 1298 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 669 CAGCTG CAG^CTG SacII 560 CCGCGG CCGC^GG SfiI 1992 GGCCAGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1300 CTCGAG C^T[CT][GA]AG SspI 616 AATATT AAT^ATT StuI 1850 AGGCCT AGG^CCT TaqI 1301 TCGA T^CGA TthHB8I 1301 TCGA T^CGA XhoI 1300 CTCGAG C^TCGAG XmnI 1759 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 355 TCCGGA T^CCGGA 586 TCCGGA ApaI 27 GGGCCC GGGCC^C 820 GGGCCC BalI 131 TGGCCA TGG^CCA 1991 TGGCCA BglI 1704 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1993 GCCAGTCTGGC BlpI 543 GCTGAGC GC^T[ACGT]AGC 1113 GCTCAGC Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC 1113 GCTCAGC BsaWI 355 TCCGGA [AT]^CCGG[AT] 586 TCCGGA BseAI 355 TCCGGA T^CCGGA 586 TCCGGA BspEI 355 TCCGGA T^CCGGA 586 TCCGGA BspHI 1055 TCATGA T^CATGA 1492 TCATGA BsrDI 742 GCAATG GCAATG(2/0) 1659 GCAATG BsrFI 514 GCCGGC [GA]^CCGG[CT] 1234 ACCGGC BstYI 352 AGATCC [GA]^GATC[CT] 1922 GGATCT CelII 543 GCTGAGC GC^T[ACGT]AGC 1113 GCTCAGC Cfr10I 514 GCCGGC [GA]^CCGG[CT] 1234 ACCGGC EcoNI 880 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2023 CCTGGGGTAGG HaeII 547 AGCGCT [GA]GCGC^[CT] 1972 GGCGCT HincII 215 GTTGAC GT[CT]^[GA]AC 1287 GTCAAC HindII 215 GTTGAC GT[CT]^[GA]AC 1287 GTCAAC MflI 352 AGATCC [GA]^GATC[CT] 1922 GGATCT MluNI 131 TGGCCA TGG^CCA 1991 TGGCCA MroI 355 TCCGGA T^CCGGA 586 TCCGGA MscI 131 TGGCCA TGG^CCA 1991 TGGCCA RcaI 1055 TCATGA T^CATGA 1492 TCATGA SapI 1263 GAAGAGC GCTCTTC(1/4) 749 GCTCTTC TfiI 590 GATTC G^A[AT]TC 1024 GATTC XcmI 994 CCACACTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1716 CCAGCCCCAGACTGG XhoII 352 AGATCC [GA]^GATC[CT] 1922 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### Acc65I AciI AfaI AluI AlwI AlwNI AocI Asp718I AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I