##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AhdI Alw44I AlwNI Aor51HI
ApaLI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BbvCI BclI BfaI BglII BlnI
BlpI Bpu1102I BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I CelII
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FbaI FseI KpnI KspI
MaeI MfeI MluI MroI MunI
NheI NotI NruI PacI PflFI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 1941 GTAC GT^AC
AflII 2090 CTTAAG C^TTAAG
AgeI 2340 ACCGGT A^CCGGT
AocI 105 CCTAAGG CC^T[ACGT]AGG
AscI 66 GGCGCGCC GG^CGCGCC
AviII 2278 TGCGCA TGC^GCA
BbrPI 1933 CACGTG CAC^GTG
BcgI 1367 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 491 GTATCC GTATCC(6/5)
BfrI 2090 CTTAAG C^TTAAG
BsaAI 1933 CACGTG [CT]AC^GT[GA]
BsaI 2122 GAGACC GGTCTC(1/5)
BsaWI 2340 ACCGGT [AT]^CCGG[AT]
BsiEI 1404 CGACCG CG[GA][CT]^CG
BsmAI 2122 GAGAC GTCTC(1/5)
BspDI 2114 ATCGAT AT^CGAT
BssHII 67 GCGCGC G^CGCGC
BstBI 2072 TTCGAA TT^CGAA
Bsu36I 105 CCTAAGG CC^T[ACGT]AGG
ClaI 2114 ATCGAT AT^CGAT
Eco81I 105 CCTAAGG CC^T[ACGT]AGG
EcoRI 298 GAATTC G^AATTC
EcoT22I 530 ATGCAT ATGCA^T
FspI 2278 TGCGCA TGC^GCA
HindIII 1797 AAGCTT A^AGCTT
HinfI 1999 GAGTC G^A[ACGT]TC
HpaI 2149 GTTAAC GTT^AAC
NdeI 187 CATATG CA^TATG
NsiI 530 ATGCAT ATGCA^T
NspI 399 ACATGC [GA]CATG^[CT]
NspV 2072 TTCGAA TT^CGAA
PinAI 2340 ACCGGT A^CCGGT
PleI 1999 GAGTC GAGTC(4/5)
PmaCI 1933 CACGTG CAC^GTG
PmlI 1933 CACGTG CAC^GTG
PvuII 745 CAGCTG CAG^CTG
RsaI 1941 GTAC GT^AC
SfuI 2072 TTCGAA TT^CGAA
StuI 1853 AGGCCT AGG^CCT
Tsp45I 254 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1576 GGCGCC G[GA]^CG[CT]C
1615 GGCGCC
BbeI 1576 GGCGCC GGCGC^C
1615 GGCGCC
BbsI 362 GAAGAC GAAGAC(2/6)
545 GAAGAC
BbvI 328 GCTGC GCAGC(8/12)
747 GCTGC
BglI 1274 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1846 GCCAGGAAGGC
BpuAI 362 GAAGAC GAAGAC(2/6)
545 GAAGAC
BsaHI 1576 GGCGCC G[GA]^CG[CT]C
1615 GGCGCC
BstYI 384 AGATCC [GA]^GATC[CT]
991 GGATCT
DraI 2107 TTTAAA TTT^AAA
2188 TTTAAA
HgaI 822 GCGTC GACGC(5/10)
1091 GCGTC
Hin1I 1576 GGCGCC G[GA]^CG[CT]C
1615 GGCGCC
HincII 2149 GTTAAC GT[CT]^[GA]AC
2309 GTTGAC
HindII 2149 GTTAAC GT[CT]^[GA]AC
2309 GTTGAC
KasI 1576 GGCGCC G^GCGCC
1615 GGCGCC
MaeIII 254 GTCAC ^GT[ACGT]AC
691 GTTAC
MflI 384 AGATCC [GA]^GATC[CT]
991 GGATCT
MspA1I 745 CAGCTG C[AC]G^C[GT]G
1276 CCGCTG
NaeI 1141 GCCGGC GCC^GGC
1363 GCCGGC
NarI 1576 GGCGCC GG^CGCC
1615 GGCGCC
NgoMIV 1141 GCCGGC G^CCGGC
1363 GCCGGC
PaeR7I 1627 CTCGAG C^TCGAG
1666 CTCGAG
PflMI 1173 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1320 CCAGACCATGG
PpuMI 933 AGGTCCC [GA]G^G[AT]CC[CT]
1113 GGGTCCT
SexAI 265 ACCTGGT A^CC[AT]GGT
456 ACCAGGT
SfiI 1273 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1845 GGCCAGGAAGGCC
SfoI 1576 GGCGCC GGC^GCC
1615 GGCGCC
TseI 328 GCTGC G^C[AT]GC
747 GCTGC
Van91I 1173 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1320 CCAGACCATGG
XhoI 1627 CTCGAG C^TCGAG
1666 CTCGAG
XhoII 384 AGATCC [GA]^GATC[CT]
991 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApaI ApoI AvaI AvaII BalI
BanI BanII BcnI BmyI BpmI
BsaBI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MamI
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I Tsp509I
TspRI TthHB8I XcmI