################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AhdI Alw44I AlwNI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbvCI BclI BfaI BglII BlnI BlpI Bpu1102I BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FbaI FseI KpnI KspI MaeI MfeI MluI MroI MunI NheI NotI NruI PacI PflFI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 1941 GTAC GT^AC AflII 2090 CTTAAG C^TTAAG AgeI 2340 ACCGGT A^CCGGT AocI 105 CCTAAGG CC^T[ACGT]AGG AscI 66 GGCGCGCC GG^CGCGCC AviII 2278 TGCGCA TGC^GCA BbrPI 1933 CACGTG CAC^GTG BcgI 1367 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 491 GTATCC GTATCC(6/5) BfrI 2090 CTTAAG C^TTAAG BsaAI 1933 CACGTG [CT]AC^GT[GA] BsaI 2122 GAGACC GGTCTC(1/5) BsaWI 2340 ACCGGT [AT]^CCGG[AT] BsiEI 1404 CGACCG CG[GA][CT]^CG BsmAI 2122 GAGAC GTCTC(1/5) BspDI 2114 ATCGAT AT^CGAT BssHII 67 GCGCGC G^CGCGC BstBI 2072 TTCGAA TT^CGAA Bsu36I 105 CCTAAGG CC^T[ACGT]AGG ClaI 2114 ATCGAT AT^CGAT Eco81I 105 CCTAAGG CC^T[ACGT]AGG EcoRI 298 GAATTC G^AATTC EcoT22I 530 ATGCAT ATGCA^T FspI 2278 TGCGCA TGC^GCA HindIII 1797 AAGCTT A^AGCTT HinfI 1999 GAGTC G^A[ACGT]TC HpaI 2149 GTTAAC GTT^AAC NdeI 187 CATATG CA^TATG NsiI 530 ATGCAT ATGCA^T NspI 399 ACATGC [GA]CATG^[CT] NspV 2072 TTCGAA TT^CGAA PinAI 2340 ACCGGT A^CCGGT PleI 1999 GAGTC GAGTC(4/5) PmaCI 1933 CACGTG CAC^GTG PmlI 1933 CACGTG CAC^GTG PvuII 745 CAGCTG CAG^CTG RsaI 1941 GTAC GT^AC SfuI 2072 TTCGAA TT^CGAA StuI 1853 AGGCCT AGG^CCT Tsp45I 254 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 1576 GGCGCC G[GA]^CG[CT]C 1615 GGCGCC BbeI 1576 GGCGCC GGCGC^C 1615 GGCGCC BbsI 362 GAAGAC GAAGAC(2/6) 545 GAAGAC BbvI 328 GCTGC GCAGC(8/12) 747 GCTGC BglI 1274 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1846 GCCAGGAAGGC BpuAI 362 GAAGAC GAAGAC(2/6) 545 GAAGAC BsaHI 1576 GGCGCC G[GA]^CG[CT]C 1615 GGCGCC BstYI 384 AGATCC [GA]^GATC[CT] 991 GGATCT DraI 2107 TTTAAA TTT^AAA 2188 TTTAAA HgaI 822 GCGTC GACGC(5/10) 1091 GCGTC Hin1I 1576 GGCGCC G[GA]^CG[CT]C 1615 GGCGCC HincII 2149 GTTAAC GT[CT]^[GA]AC 2309 GTTGAC HindII 2149 GTTAAC GT[CT]^[GA]AC 2309 GTTGAC KasI 1576 GGCGCC G^GCGCC 1615 GGCGCC MaeIII 254 GTCAC ^GT[ACGT]AC 691 GTTAC MflI 384 AGATCC [GA]^GATC[CT] 991 GGATCT MspA1I 745 CAGCTG C[AC]G^C[GT]G 1276 CCGCTG NaeI 1141 GCCGGC GCC^GGC 1363 GCCGGC NarI 1576 GGCGCC GG^CGCC 1615 GGCGCC NgoMIV 1141 GCCGGC G^CCGGC 1363 GCCGGC PaeR7I 1627 CTCGAG C^TCGAG 1666 CTCGAG PflMI 1173 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1320 CCAGACCATGG PpuMI 933 AGGTCCC [GA]G^G[AT]CC[CT] 1113 GGGTCCT SexAI 265 ACCTGGT A^CC[AT]GGT 456 ACCAGGT SfiI 1273 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1845 GGCCAGGAAGGCC SfoI 1576 GGCGCC GGC^GCC 1615 GGCGCC TseI 328 GCTGC G^C[AT]GC 747 GCTGC Van91I 1173 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1320 CCAGACCATGG XhoI 1627 CTCGAG C^TCGAG 1666 CTCGAG XhoII 384 AGATCC [GA]^GATC[CT] 991 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApaI ApoI AvaI AvaII BalI BanI BanII BcnI BmyI BpmI BsaBI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MamI MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I Tsp509I TspRI TthHB8I XcmI