##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstPI BstXI CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI HapII
Hin1I HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MspI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 76 CGCG CG^CG
AflIII 697 ACGTGT A^C[GA][CT]GT
AocI 1638 CCTGAGG CC^T[ACGT]AGG
ApaI 111 GGGCCC GGGCC^C
AseI 870 ATTAAT AT^TAAT
AsnI 870 ATTAAT AT^TAAT
AspHI 640 GAGCAC G[AT]GC[AT]^C
AvaI 37 CCCGAG C^[CT]CG[GA]G
AviII 934 TGCGCA TGC^GCA
BanII 111 GGGCCC G[GA]GC[CT]^C
BclI 1120 TGATCA T^GATCA
BglI 27 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 148 CTGGAG CTGGAG(16/14)
BsaBI 566 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2144 GAGGAG GAGGAG(10/8)
BsiHKAI 640 GAGCAC G[AT]GC[AT]^C
BsoBI 37 CCCGAG C^[CT]CG[GA]G
BspHI 562 TCATGA T^CATGA
BspMI 1797 GCAGGT ACCTGC(4/8)
BstAPI 86 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 76 CGCG CG^CG
BstYI 314 GGATCT [GA]^GATC[CT]
Bsu36I 1638 CCTGAGG CC^T[ACGT]AGG
Eco81I 1638 CCTGAGG CC^T[ACGT]AGG
FbaI 1120 TGATCA T^GATCA
FspI 934 TGCGCA TGC^GCA
HgaI 2332 GCGTC GACGC(5/10)
MamI 566 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 314 GGATCT [GA]^GATC[CT]
MspA1I 1649 CAGCTG C[AC]G^C[GT]G
MvnI 76 CGCG CG^CG
PflMI 1221 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1008 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 870 ATTAAT AT^TAAT
PvuII 1649 CAGCTG CAG^CTG
RcaI 562 TCATGA T^CATGA
SfiI 26 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 678 GCATGC GCATG^C
StuI 332 AGGCCT AGG^CCT
Van91I 1221 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 314 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1052 GTATAC GT^[AC][GT]AC
1784 GTATAC
AfaI 268 GTAC GT^AC
466 GTAC
AlwNI 502 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1222 CAGGTGCTG
Asp700I 330 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1570 GAATGTCTTC
BalI 448 TGGCCA TGG^CCA
526 TGGCCA
BanI 120 GGCACC G^G[CT][GA]CC
1230 GGTGCC
BsgI 1517 CTGCAC GTGCAG(16/14)
751 GTGCAG
BsmAI 151 GAGAC GTCTC(1/5)
2325 GTCTC
BsmFI 1028 GGGAC GGGAC(10/14)
1171 GGGAC
BssSI 2364 CACGAG CACGAG(-5/-1)
771 CTCGTG
Bst1107I 1052 GTATAC GTA^TAC
1784 GTATAC
BstZ17I 1052 GTATAC GTA^TAC
1784 GTATAC
DraII 110 GGGGCCC [GA]G^G[ACGT]CC[CT]
382 AGGCCCT
EaeI 448 TGGCCA [CT]^GGCC[GA]
526 TGGCCA
EcoO109I 110 GGGGCCC [GA]G^G[ACGT]CC[CT]
382 AGGCCCT
EcoRI 728 GAATTC G^AATTC
995 GAATTC
FauI 2116 CCCGC CCCGC(4/6)
77 GCGGG
HaeII 50 GGCGCT [GA]GCGC^[CT]
144 GGCGCT
HincII 60 GTCAAC GT[CT]^[GA]AC
2149 GTCAAC
HindII 60 GTCAAC GT[CT]^[GA]AC
2149 GTCAAC
MluNI 448 TGGCCA TGG^CCA
526 TGGCCA
MscI 448 TGGCCA TGG^CCA
526 TGGCCA
NdeI 1700 CATATG CA^TATG
1868 CATATG
NspI 678 GCATGC [GA]CATG^[CT]
1031 ACATGC
PstI 84 CTGCAG CTGCA^G
2396 CTGCAG
RsaI 268 GTAC GT^AC
466 GTAC
SmlI 1178 CTTGAG C^T[CT][GA]AG
2007 CTCAAG
XcmI 22 CCAAGGCCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1534 CCAACATTCTCTTGG
XmnI 330 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1570 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NsiI
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI