##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI AviII
AvrII BbeI BbrPI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 1540 GGCGTC G[GA]^CG[CT]C
AhdI 1381 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1381 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 247 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1450 GGTGCC G^G[CT][GA]CC
BanII 1194 GGGCTC G[GA]GC[CT]^C
BbsI 583 GAAGAC GAAGAC(2/6)
BcgI 162 CGACTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 458 GTATCC GTATCC(6/5)
BclI 893 TGATCA T^GATCA
BfaI 739 CTAG C^TAG
BglI 1168 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 513 CTCCAG CTGGAG(16/14)
BpuAI 583 GAAGAC GAAGAC(2/6)
BsaAI 70 TACGTG [CT]AC^GT[GA]
BsaHI 1540 GGCGTC G[GA]^CG[CT]C
BsaWI 1113 TCCGGT [AT]^CCGG[AT]
BsgI 1375 GTGCAG GTGCAG(16/14)
BsmBI 116 GAGACG CGTCTC(1/5)
BspMI 982 GCAGGT ACCTGC(4/8)
BsrI 228 ACTGG ACTGG(1/-1)
BstAPI 1035 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 385 GACCCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1381 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 328 GAATTC G^AATTC
FbaI 893 TGATCA T^GATCA
HgaI 1541 GCGTC GACGC(5/10)
Hin1I 1540 GGCGTC G[GA]^CG[CT]C
MaeI 739 CTAG C^TAG
MspA1I 763 CAGCTG C[AC]G^C[GT]G
PflFI 247 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1137 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1464 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 763 CAGCTG CAG^CTG
SapI 102 GCTCTTC GCTCTTC(1/4)
SbfI 601 CCTGCAGG CCTGCA^GG
SexAI 176 ACCAGGT A^CC[AT]GGT
SfiI 1167 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 601 CCTGCAGG CCTGCA^GG
Tth111I 247 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1137 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 217 CTTAAG C^TTAAG
1498 CTTAAG
AvaII 183 GGACC G^G[AT]CC
393 GGTCC
BamHI 1250 GGATCC G^GATCC
1586 GGATCC
BcnI 440 CCCGG CC^[GC]GG
704 CCCGG
BfrI 217 CTTAAG C^TTAAG
1498 CTTAAG
BmyI 60 GGGCAC G[AGT]GC[ACT]^C
1194 GGGCTC
BsmAI 8 GAGAC GTCTC(1/5)
116 GAGAC
Bsp1286I 60 GGGCAC G[AGT]GC[ACT]^C
1194 GGGCTC
Bsp1407I 319 TGTACA T^GTACA
1076 TGTACA
BspHI 792 TCATGA T^CATGA
1187 TCATGA
BsrGI 319 TGTACA T^GTACA
1076 TGTACA
BstYI 1250 GGATCC [GA]^GATC[CT]
1586 GGATCC
DraII 1174 GGGGCCT [GA]G^G[ACGT]CC[CT]
1220 AGGCCCC
EcoO109I 1174 GGGGCCT [GA]G^G[ACGT]CC[CT]
1220 AGGCCCC
FauI 417 GCGGG CCCGC(4/6)
999 GCGGG
HaeII 282 GGCGCT [GA]GCGC^[CT]
1100 GGCGCT
HphI 1228 GGTGA GGTGA(8/7)
125 TCACC
MaeIII 253 GTCAC ^GT[ACGT]AC
949 GTCAC
MflI 1250 GGATCC [GA]^GATC[CT]
1586 GGATCC
MseI 218 TTAA T^TAA
1499 TTAA
NciI 440 CCCGG CC^[GC]GG
704 CCCGG
PleI 86 GACTC GAGTC(4/5)
856 GACTC
RcaI 792 TCATGA T^CATGA
1187 TCATGA
SmlI 217 CTTAAG C^T[CT][GA]AG
1498 CTTAAG
SspBI 319 TGTACA T^GTACA
1076 TGTACA
TaqI 161 TCGA T^CGA
1286 TCGA
Tru9I 218 TTAA T^TAA
1499 TTAA
Tsp45I 253 GTCAC ^GT[GC]AC
949 GTCAC
TthHB8I 161 TCGA T^CGA
1286 TCGA
XhoII 1250 GGATCC [GA]^GATC[CT]
1586 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI BalI BbvCI
BbvI BsaJI BseRI BsiYI BslI
BsmFI BsrDI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp509I TspRI XcmI