##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AcsI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvrII BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII KpnI KspI MfeI
MluI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 1497 TCCGGA T^CCGGA
AclI 660 AACGTT AA^CGTT
AocI 1009 CCTGAGG CC^T[ACGT]AGG
Asp700I 1393 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 600 CTCGAG C^[CT]CG[GA]G
AvaII 612 GGACC G^G[AT]CC
AviII 1019 TGCGCA TGC^GCA
BamHI 1274 GGATCC G^GATCC
BbeI 106 GGCGCC GGCGC^C
BbrPI 1332 CACGTG CAC^GTG
BbsI 1358 GAAGAC GAAGAC(2/6)
BfaI 1386 CTAG C^TAG
BglII 1550 AGATCT A^GATCT
BpuAI 1358 GAAGAC GAAGAC(2/6)
BsaAI 1332 CACGTG [CT]AC^GT[GA]
BsaBI 1233 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1497 TCCGGA [AT]^CCGG[AT]
BseAI 1497 TCCGGA T^CCGGA
BseRI 1000 GAGGAG GAGGAG(10/8)
BsmFI 1101 GTCCC GGGAC(10/14)
BsoBI 600 CTCGAG C^[CT]CG[GA]G
BspDI 1111 ATCGAT AT^CGAT
BspEI 1497 TCCGGA T^CCGGA
BspHI 1199 TCATGA T^CATGA
BstXI 1206 CCACTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1009 CCTGAGG CC^T[ACGT]AGG
ClaI 1111 ATCGAT AT^CGAT
DsaI 1166 CCATGG C^C[GA][CT]GG
Eco81I 1009 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1166 CCATGG C^C[AT][AT]GG
FspI 1019 TGCGCA TGC^GCA
HpaI 640 GTTAAC GTT^AAC
KasI 106 GGCGCC G^GCGCC
MaeI 1386 CTAG C^TAG
MamI 1233 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1497 TCCGGA T^CCGGA
NaeI 649 GCCGGC GCC^GGC
NarI 106 GGCGCC GG^CGCC
NcoI 1166 CCATGG C^CATGG
NgoMIV 649 GCCGGC G^CCGGC
PaeR7I 600 CTCGAG C^TCGAG
PflMI 1161 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1332 CACGTG CAC^GTG
PmlI 1332 CACGTG CAC^GTG
PpuMI 611 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 239 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 660 AACGTT AA^CGTT
RcaI 1199 TCATGA T^CATGA
ScaI 1040 AGTACT AGT^ACT
SfiI 816 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 106 GGCGCC GGC^GCC
SmlI 600 CTCGAG C^T[CT][GA]AG
SphI 800 GCATGC GCATG^C
StuI 1457 AGGCCT AGG^CCT
StyI 1166 CCATGG C^C[AT][AT]GG
TfiI 1486 GAATC G^A[AT]TC
Tsp509I 1506 AATT ^AATT
Van91I 1161 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 600 CTCGAG C^TCGAG
XmnI 1393 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 103 CGCG CG^CG
781 CGCG
AcyI 106 GGCGCC G[GA]^CG[CT]C
299 GACGCC
AflIII 1097 ACACGT A^C[GA][CT]GT
1333 ACGTGT
BanI 95 GGCACC G^G[CT][GA]CC
106 GGCGCC
BcgI 133 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
932 GCAACTTCATCG
BglI 355 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
817 GCCAACCCGGC
BsaHI 106 GGCGCC G[GA]^CG[CT]C
299 GACGCC
BsaI 691 GAGACC GGTCTC(1/5)
1069 GAGACC
BspMI 98 ACCTGC ACCTGC(4/8)
702 GCAGGT
BsrFI 369 GCCGGT [GA]^CCGG[CT]
649 GCCGGC
BstUI 103 CGCG CG^CG
781 CGCG
BstYI 1274 GGATCC [GA]^GATC[CT]
1550 AGATCT
Cfr10I 369 GCCGGT [GA]^CCGG[CT]
649 GCCGGC
DraII 611 AGGACCC [GA]G^G[ACGT]CC[CT]
632 GGGGCCT
EarI 568 GAAGAG CTCTTC(1/4)
1247 GAAGAG
EcoNI 616 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1264 CCTCTGTCAGG
EcoO109I 611 AGGACCC [GA]G^G[ACGT]CC[CT]
632 GGGGCCT
HaeII 61 AGCGCC [GA]GCGC^[CT]
106 GGCGCC
HgaI 299 GACGC GACGC(5/10)
782 GCGTC
Hin1I 106 GGCGCC G[GA]^CG[CT]C
299 GACGCC
HincII 640 GTTAAC GT[CT]^[GA]AC
1315 GTCAAC
HindII 640 GTTAAC GT[CT]^[GA]AC
1315 GTCAAC
Ksp632I 568 GAAGAG CTCTTC(1/4)
1247 GAAGAG
MflI 1274 GGATCC [GA]^GATC[CT]
1550 AGATCT
MspA1I 144 CAGCGG C[AC]G^C[GT]G
1242 CCGCTG
MvnI 103 CGCG CG^CG
781 CGCG
NspI 626 GCATGT [GA]CATG^[CT]
800 GCATGC
PleI 406 GACTC GAGTC(4/5)
1250 GAGTC
TaqI 601 TCGA T^CGA
1112 TCGA
TthHB8I 601 TCGA T^CGA
1112 TCGA
XhoII 1274 GGATCC [GA]^GATC[CT]
1550 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I TspRI