##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BanI BbeI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaI BseRI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI HphI KasI KpnI Ksp632I
KspI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PaeR7I
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1534 GTCTAC GT^[AC][GT]AC
AccIII 993 TCCGGA T^CCGGA
AcyI 1013 GACGCC G[GA]^CG[CT]C
AflII 169 CTTAAG C^TTAAG
AflIII 120 ACACGT A^C[GA][CT]GT
AlwNI 801 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 281 ATTAAT AT^TAAT
AsnI 281 ATTAAT AT^TAAT
Asp700I 1602 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 124 GTGCTC G[AT]GC[AT]^C
AspI 1344 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 512 GGATCC G^GATCC
BanII 1123 GAGCCC G[GA]GC[CT]^C
BbrPI 121 CACGTG CAC^GTG
BcnI 907 CCGGG CC^[GC]GG
BfrI 169 CTTAAG C^TTAAG
BsaAI 121 CACGTG [CT]AC^GT[GA]
BsaBI 803 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1013 GACGCC G[GA]^CG[CT]C
BsaWI 993 TCCGGA [AT]^CCGG[AT]
BseAI 993 TCCGGA T^CCGGA
BsgI 855 CTGCAC GTGCAG(16/14)
BsiHKAI 124 GTGCTC G[AT]GC[AT]^C
BsiYI 1066 CCAAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1066 CCAAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 31 GAGACG CGTCTC(1/5)
BsmI 611 GAATGC GAATGC(1/-1)
BspEI 993 TCCGGA T^CCGGA
BspHI 235 TCATGA T^CATGA
BssSI 1432 CTCGTG CACGAG(-5/-1)
DsaI 1218 CCATGG C^C[GA][CT]GG
FauI 5 GCGGG CCCGC(4/6)
Hin1I 1013 GACGCC G[GA]^CG[CT]C
HindIII 673 AAGCTT A^AGCTT
MamI 803 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 709 CAATTG C^AATTG
MroI 993 TCCGGA T^CCGGA
MunI 709 CAATTG C^AATTG
NciI 907 CCGGG CC^[GC]GG
NcoI 1218 CCATGG C^CATGG
PacI 278 TTAATTAA TTAAT^TAA
PflFI 1344 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1344 GACTC GAGTC(4/5)
PmaCI 121 CACGTG CAC^GTG
PmlI 121 CACGTG CAC^GTG
PshBI 281 ATTAAT AT^TAAT
RcaI 235 TCATGA T^CATGA
SspI 985 AATATT AAT^ATT
Tsp45I 915 GTGAC ^GT[GC]AC
Tth111I 1344 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1602 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 35 CGCG CG^CG
115 CGCG
AcsI 1236 GAATTT [GA]^AATT[CT]
1507 AAATTC
ApoI 1236 GAATTT [GA]^AATT[CT]
1507 AAATTC
BmyI 124 GTGCTC G[AGT]GC[ACT]^C
1123 GAGCCC
BpmI 790 CTCCAG CTGGAG(16/14)
28 CTGGAG
BsmAI 31 GAGAC GTCTC(1/5)
1708 GAGAC
Bsp1286I 124 GTGCTC G[AGT]GC[ACT]^C
1123 GAGCCC
BsrDI 745 CATTGC GCAATG(2/0)
156 GCAATG
BstUI 35 CGCG CG^CG
115 CGCG
Cac8I 663 GCAAGC GC[ACGT]^[ACGT]GC
1022 GCGTGC
EcoT14I 720 CCTTGG C^C[AT][AT]GG
1218 CCATGG
HapII 907 CCGG C^CGG
994 CCGG
HpaII 907 CCGG C^CGG
994 CCGG
MaeIII 838 GTTAC ^GT[ACGT]AC
915 GTGAC
MspI 907 CCGG C^CGG
994 CCGG
MvnI 35 CGCG CG^CG
115 CGCG
MwoI 1251 GCAATTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1437 GCCTCAAACGC
StyI 720 CCTTGG C^C[AT][AT]GG
1218 CCATGG
XcmI 517 CCATTCTATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
616 CCAGGATGACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BfaI BpuAI BsaJI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HgaI HinfI
ItaI MaeI MaeII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII