##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbeI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 357 GTAGAC GT^[AC][GT]AC
AlwNI 208 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 798 CCTGAGG CC^T[ACGT]AGG
BalI 575 TGGCCA TGG^CCA
BamHI 895 GGATCC G^GATCC
BanI 333 GGTGCC G^G[CT][GA]CC
BbrPI 1559 CACGTG CAC^GTG
BbsI 1121 GTCTTC GAAGAC(2/6)
BciVI 1554 GGATAC GTATCC(6/5)
BpuAI 1121 GTCTTC GAAGAC(2/6)
BsaAI 1559 CACGTG [CT]AC^GT[GA]
BseRI 170 GAGGAG GAGGAG(10/8)
BsgI 1533 CTGCAC GTGCAG(16/14)
BspHI 1447 TCATGA T^CATGA
BsrFI 186 ACCGGC [GA]^CCGG[CT]
Bsu36I 798 CCTGAGG CC^T[ACGT]AGG
CfoI 978 GCGC GCG^C
Cfr10I 186 ACCGGC [GA]^CCGG[CT]
DraI 923 TTTAAA TTT^AAA
DraII 782 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 572 CCATGG C^C[GA][CT]GG
EaeI 575 TGGCCA [CT]^GGCC[GA]
Eco81I 798 CCTGAGG CC^T[ACGT]AGG
EcoO109I 782 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 822 ATGCAT ATGCA^T
HaeIII 576 GGCC GG^CC
HapII 187 CCGG C^CGG
HhaI 978 GCGC GCG^C
HinP1I 978 GCGC G^CGC
HindIII 153 AAGCTT A^AGCTT
HpaII 187 CCGG C^CGG
MaeII 1560 ACGT A^CGT
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MspI 187 CCGG C^CGG
NcoI 572 CCATGG C^CATGG
NdeI 1164 CATATG CA^TATG
NsiI 822 ATGCAT ATGCA^T
PmaCI 1559 CACGTG CAC^GTG
PmlI 1559 CACGTG CAC^GTG
PpuMI 782 GGGACCC [GA]G^G[AT]CC[CT]
PstI 362 CTGCAG CTGCA^G
RcaI 1447 TCATGA T^CATGA
SexAI 1013 ACCAGGT A^CC[AT]GGT
SgrAI 185 CACCGGCG C[GA]^CCGG[CT]G
TaqI 7 TCGA T^CGA
TfiI 830 GATTC G^A[AT]TC
TthHB8I 7 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 617 GGTCC G^G[AT]CC
783 GGACC
BpmI 300 CTGGAG CTGGAG(16/14)
709 CTGGAG
BsaJI 337 CCAAGG C^C[ACGT][ACGT]GG
572 CCATGG
BsmAI 230 GAGAC GTCTC(1/5)
612 GTCTC
BspLU11I 608 ACATGT A^CATGT
723 ACATGT
BsrDI 1481 CATTGC GCAATG(2/0)
555 GCAATG
BssKI 914 CCTGG ^CC[ACGT]GG
1014 CCAGG
BstNI 914 CCTGG CC^[AT]GG
1014 CCAGG
BstYI 482 AGATCC [GA]^GATC[CT]
895 GGATCC
Cac8I 65 GCTCGC GC[ACGT]^[ACGT]GC
265 GCAAGC
Cfr13I 617 GGTCC G^G[ACGT]CC
783 GGACC
EcoRII 914 CCTGG ^CC[AT]GG
1014 CCAGG
EcoT14I 337 CCAAGG C^C[AT][AT]GG
572 CCATGG
MflI 482 AGATCC [GA]^GATC[CT]
895 GGATCC
MslI 724 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1513 CATTCCAATG
MspA1I 310 CAGCTG C[AC]G^C[GT]G
601 CAGCTG
MvaI 914 CCTGG CC^[AT]GG
1014 CCAGG
MwoI 594 GCTTCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
978 GCGCAATAAGC
NspI 608 ACATGT [GA]CATG^[CT]
723 ACATGT
PleI 141 GAGTC GAGTC(4/5)
712 GAGTC
PvuII 310 CAGCTG CAG^CTG
601 CAGCTG
Sau96I 617 GGTCC G^G[ACGT]CC
783 GGACC
ScrFI 914 CCTGG CC^[ACGT]GG
1014 CCAGG
SfcI 362 CTGCAG C^T[GA][CT]AG
868 CTATAG
StyI 337 CCAAGG C^C[AT][AT]GG
572 CCATGG
XhoII 482 AGATCC [GA]^GATC[CT]
895 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI BbvI BfaI BsiYI BslI
BsmFI BsrI BstF5I DdeI DpnII
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NlaIV RsaI
Sau3AI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI