##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
ApoI AseI AsnI Asp718I AspEI
AspI AvaI AviII BamHI BanI
BbeI BbsI BclI BfrI BpuAI
BsaHI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BstBI
BstEII BstPI ClaI CpoI DraI
Eam1105I EcoO65I EcoRI EcoRV FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AocI 2353 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1280 AGCGCT AGC^GCT
AscI 1475 GGCGCGCC GG^CGCGCC
AvrII 2000 CCTAGG C^CTAGG
BbrPI 2284 CACGTG CAC^GTG
BcgI 203 GCAGACACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1546 GGATAC GTATCC(6/5)
BglII 722 AGATCT A^GATCT
BlnI 2000 CCTAGG C^CTAGG
BlpI 372 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 372 GCTCAGC GC^T[ACGT]AGC
BsaBI 1371 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 860 ACCGGA [AT]^CCGG[AT]
Bsp1407I 387 TGTACA T^GTACA
BsrFI 497 ACCGGC [GA]^CCGG[CT]
BsrGI 387 TGTACA T^GTACA
BssHII 1476 GCGCGC G^CGCGC
BssSI 733 CACGAG CACGAG(-5/-1)
Bst1107I 1081 GTATAC GTA^TAC
BstZ17I 1081 GTATAC GTA^TAC
Bsu36I 2353 CCTGAGG CC^T[ACGT]AGG
CelII 372 GCTCAGC GC^T[ACGT]AGC
Cfr10I 497 ACCGGC [GA]^CCGG[CT]
DrdI 1099 GACAACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1386 CGGCCG C^GGCCG
EclXI 1386 CGGCCG C^GGCCG
Eco47III 1280 AGCGCT AGC^GCT
Eco52I 1386 CGGCCG C^GGCCG
Eco81I 2353 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2375 ATGCAT ATGCA^T
MamI 1371 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 1385 GCGGCCGC GC^GGCCGC
NsiI 2375 ATGCAT ATGCA^T
NspI 1043 GCATGT [GA]CATG^[CT]
PleI 1126 GAGTC GAGTC(4/5)
PmaCI 2284 CACGTG CAC^GTG
PmlI 2284 CACGTG CAC^GTG
PshAI 1595 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1565 ACCAGGT A^CC[AT]GGT
SspBI 387 TGTACA T^GTACA
StuI 1782 AGGCCT AGG^CCT
TfiI 170 GAATC G^A[AT]TC
Tsp509I 432 AATT ^AATT
XcmI 1775 CCATCTCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1561 GTGCAC G^TGCAC
1717 GTGCAC
ApaLI 1561 GTGCAC G^TGCAC
1717 GTGCAC
Asp700I 1014 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2155 GAACTGCTTC
BcnI 159 CCGGG CC^[GC]GG
1866 CCCGG
BsaI 658 GAGACC GGTCTC(1/5)
648 GGTCTC
BsiEI 910 CGGTCG CG[GA][CT]^CG
1386 CGGCCG
BsmI 1269 GAATGC GAATGC(1/-1)
944 GCATTC
BsrBI 1683 CCGCTC CCGCTC(-3/-3)
1812 CCGCTC
BsrDI 1644 CATTGC GCAATG(2/0)
383 GCAATG
BstAPI 792 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2151 GCAGGAACTGC
BstXI 2081 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2439 CCATGTTGTTGG
HaeII 844 AGCGCC [GA]GCGC^[CT]
1280 AGCGCT
HgaI 1356 GACGC GACGC(5/10)
238 GCGTC
HinfI 170 GAATC G^A[ACGT]TC
1126 GAGTC
KspI 1695 CCGCGG CCGC^GG
1836 CCGCGG
MaeIII 1250 GTGAC ^GT[ACGT]AC
2145 GTCAC
MslI 1059 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1644 CATTGCTGTG
NciI 159 CCGGG CC^[GC]GG
1866 CCCGG
PflMI 1298 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGGCTGTGG
SacI 370 GAGCTC GAGCT^C
2348 GAGCTC
SacII 1695 CCGCGG CCGC^GG
1836 CCGCGG
Tsp45I 1250 GTGAC ^GT[GC]AC
2145 GTCAC
Van91I 1298 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGGCTGTGG
XmnI 1014 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2155 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BanII BbvCI BbvI BfaI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI TspRI
TthHB8I XhoII