##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AvrII BalI BanI BbeI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsgI BsiWI
Bsp1407I BsrDI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CelII CpoI DrdI
Eco81I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluNI MscI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 72 enzymes) #
###################################
AccI 1287 GTAGAC GT^[AC][GT]AC
AccIII 1377 TCCGGA T^CCGGA
AcyI 364 GACGCC G[GA]^CG[CT]C
AfaI 1333 GTAC GT^AC
AhdI 1857 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 779 GTGCAC G^TGCAC
AlwNI 1592 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 391 AGCGCT AGC^GCT
ApaI 54 GGGCCC GGGCC^C
ApaLI 779 GTGCAC G^TGCAC
Asp700I 617 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1857 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 178 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 427 TGCGCA TGC^GCA
BamHI 1387 GGATCC G^GATCC
BbrPI 2147 CACGTG CAC^GTG
BcgI 414 CGATCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2147 CACGTG [CT]AC^GT[GA]
BsaHI 364 GACGCC G[GA]^CG[CT]C
BsaI 1231 GGTCTC GGTCTC(1/5)
BseAI 1377 TCCGGA T^CCGGA
BsiEI 99 CGGCCG CG[GA][CT]^CG
BsmBI 59 CGTCTC CGTCTC(1/5)
BsmI 1625 GAATGC GAATGC(1/-1)
BspDI 585 ATCGAT AT^CGAT
BspEI 1377 TCCGGA T^CCGGA
BspHI 1551 TCATGA T^CATGA
BspLU11I 273 ACATGT A^CATGT
BsrBI 814 CCGCTC CCGCTC(-3/-3)
BssSI 1846 CACGAG CACGAG(-5/-1)
BstAPI 1255 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 822 GGTCACC G^GT[ACGT]ACC
BstPI 822 GGTCACC G^GT[ACGT]ACC
BstYI 1387 GGATCC [GA]^GATC[CT]
ClaI 585 ATCGAT AT^CGAT
DraI 2205 TTTAAA TTT^AAA
EagI 99 CGGCCG C^GGCCG
Eam1105I 1857 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 99 CGGCCG C^GGCCG
Eco47III 391 AGCGCT AGC^GCT
Eco52I 99 CGGCCG C^GGCCG
EcoNI 593 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 822 GGTCACC G^GT[ACGT]ACC
EcoRI 1830 GAATTC G^AATTC
EcoT14I 990 CCATGG C^C[AT][AT]GG
FspI 427 TGCGCA TGC^GCA
HaeII 391 AGCGCT [GA]GCGC^[CT]
Hin1I 364 GACGCC G[GA]^CG[CT]C
MfeI 516 CAATTG C^AATTG
MflI 1387 GGATCC [GA]^GATC[CT]
MluI 1063 ACGCGT A^CGCGT
MroI 1377 TCCGGA T^CCGGA
MunI 516 CAATTG C^AATTG
NaeI 97 GCCGGC GCC^GGC
NcoI 990 CCATGG C^CATGG
NgoMIV 97 GCCGGC G^CCGGC
NspI 273 ACATGT [GA]CATG^[CT]
PaeR7I 387 CTCGAG C^TCGAG
PflFI 178 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2147 CACGTG CAC^GTG
PmlI 2147 CACGTG CAC^GTG
RcaI 1551 TCATGA T^CATGA
RsaI 1333 GTAC GT^AC
SacI 924 GAGCTC GAGCT^C
SapI 1250 GAAGAGC GCTCTTC(1/4)
SspI 1372 AATATT AAT^ATT
StyI 990 CCATGG C^C[AT][AT]GG
Tth111I 178 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 759 CCAGGCCATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 387 CTCGAG C^TCGAG
XhoII 1387 GGATCC [GA]^GATC[CT]
XmnI 617 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 315 GAATTT [GA]^AATT[CT]
1830 GAATTC
AflIII 273 ACATGT A^C[GA][CT]GT
1063 ACGCGT
ApoI 315 GAATTT [GA]^AATT[CT]
1830 GAATTC
BbvCI 1650 CCTCAGC CCTCAGC(-5/-2)
1719 GCTGAGG
BfaI 1583 CTAG C^TAG
1999 CTAG
BsaWI 876 TCCGGT [AT]^CCGG[AT]
1377 TCCGGA
BseRI 591 CTCCTC GAGGAG(10/8)
1722 GAGGAG
BssHII 83 GCGCGC G^CGCGC
119 GCGCGC
BstXI 446 CCAATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCAACGGACTGG
DraIII 1121 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2073 CACAAAGTG
EarI 1984 CTCTTC CTCTTC(1/4)
1250 GAAGAG
Ksp632I 1984 CTCTTC CTCTTC(1/4)
1250 GAAGAG
MaeI 1583 CTAG C^TAG
1999 CTAG
MslI 596 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1060 CACACGCGTG
PflMI 1222 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAGGAGCTGG
PstI 31 CTGCAG CTGCA^G
1973 CTGCAG
PvuII 1073 CAGCTG CAG^CTG
2091 CAGCTG
SmaI 5 CCCGGG CCC^GGG
133 CCCGGG
SmlI 387 CTCGAG C^T[CT][GA]AG
482 CTCAAG
Van91I 1222 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAGGAGCTGG
XmaCI 5 CCCGGG C^CCGGG
133 CCCGGG
XmaI 5 CCCGGG C^CCGGG
133 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I