##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AviII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BglII BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 177 GTCGAC GT^[AC][GT]AC
AccIII 37 TCCGGA T^CCGGA
AflII 843 CTTAAG C^TTAAG
AhdI 912 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1388 GTGCAC G^TGCAC
ApaLI 1388 GTGCAC G^TGCAC
AspEI 912 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 299 CCTAGG C^CTAGG
BanI 1299 GGTGCC G^G[CT][GA]CC
BbvCI 1083 GCTGAGG CCTCAGC(-5/-2)
BfrI 843 CTTAAG C^TTAAG
BlnI 299 CCTAGG C^CTAGG
BlpI 1101 GCTAAGC GC^T[ACGT]AGC
BpmI 465 CTGGAG CTGGAG(16/14)
Bpu1102I 1101 GCTAAGC GC^T[ACGT]AGC
BsaWI 37 TCCGGA [AT]^CCGG[AT]
BseAI 37 TCCGGA T^CCGGA
BsgI 1533 CTGCAC GTGCAG(16/14)
BsmFI 240 GTCCC GGGAC(10/14)
BspEI 37 TCCGGA T^CCGGA
BspMI 1031 ACCTGC ACCTGC(4/8)
BsrFI 304 GCCGGC [GA]^CCGG[CT]
BstAPI 1412 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1101 GCTAAGC GC^T[ACGT]AGC
Cfr10I 304 GCCGGC [GA]^CCGG[CT]
DsaI 634 CCATGG C^C[GA][CT]GG
Eam1105I 912 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 888 GAAGAG CTCTTC(1/4)
FseI 303 GGCCGGCC GGCCGG^CC
HgaI 131 GACGC GACGC(5/10)
Ksp632I 888 GAAGAG CTCTTC(1/4)
MroI 37 TCCGGA T^CCGGA
NaeI 304 GCCGGC GCC^GGC
NcoI 634 CCATGG C^CATGG
NgoMIV 304 GCCGGC G^CCGGC
PflMI 441 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1053 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1311 GAGCTC GAGCT^C
SalI 177 GTCGAC G^TCGAC
SfcI 355 CTGTAG C^T[GA][CT]AG
SmaI 1156 CCCGGG CCC^GGG
SmlI 843 CTTAAG C^T[CT][GA]AG
SspI 1356 AATATT AAT^ATT
StuI 706 AGGCCT AGG^CCT
TaqI 178 TCGA T^CGA
TthHB8I 178 TCGA T^CGA
Van91I 441 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1603 TCTAGA T^CTAGA
XmaCI 1156 CCCGGG C^CCGGG
XmaI 1156 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 10 CGCG CG^CG
12 CGCG
AfaI 335 GTAC GT^AC
1364 GTAC
AlwNI 349 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
442 CAGCTGCTG
AspI 795 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACTTTGTC
AvaI 1096 CTCGGG C^[CT]CG[GA]G
1156 CCCGGG
AvaII 1059 GGTCC G^G[AT]CC
1122 GGTCC
BcnI 1156 CCCGG CC^[GC]GG
1157 CCGGG
BglI 295 GCCACCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1011 GCCACTGTGGC
BsmAI 1231 GAGAC GTCTC(1/5)
1752 GAGAC
BsoBI 1096 CTCGGG C^[CT]CG[GA]G
1156 CCCGGG
BstUI 10 CGCG CG^CG
12 CGCG
BstYI 769 AGATCC [GA]^GATC[CT]
1562 AGATCC
CfoI 11 GCGC GCG^C
43 GCGC
FauI 8 CCCGC CCCGC(4/6)
161 GCGGG
HhaI 11 GCGC GCG^C
43 GCGC
HinP1I 11 GCGC G^CGC
43 GCGC
HindIII 553 AAGCTT A^AGCTT
1147 AAGCTT
MaeII 47 ACGT A^CGT
148 ACGT
MflI 769 AGATCC [GA]^GATC[CT]
1562 AGATCC
MspA1I 95 CAGCTG C[AC]G^C[GT]G
442 CAGCTG
MvnI 10 CGCG CG^CG
12 CGCG
NciI 1156 CCCGG CC^[GC]GG
1157 CCGGG
PflFI 795 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACTTTGTC
PleI 175 GAGTC GAGTC(4/5)
643 GAGTC
PvuII 95 CAGCTG CAG^CTG
442 CAGCTG
RsaI 335 GTAC GT^AC
1364 GTAC
ScaI 334 AGTACT AGT^ACT
1363 AGTACT
TfiI 75 GAATC G^A[AT]TC
1460 GATTC
Tth111I 795 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACTTTGTC
XcmI 1165 CCAAAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1280 CCATGTGAGCACTGG
XhoII 769 AGATCC [GA]^GATC[CT]
1562 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BalI BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I TspRI