##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflIII      
AgeI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BglII       BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseRI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HaeII       Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MslI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          177  GTCGAC               GT^[AC][GT]AC
AccIII         37  TCCGGA               T^CCGGA
AflII         843  CTTAAG               C^TTAAG
AhdI          912  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1388  GTGCAC               G^TGCAC
ApaLI        1388  GTGCAC               G^TGCAC
AspEI         912  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         299  CCTAGG               C^CTAGG
BanI         1299  GGTGCC               G^G[CT][GA]CC
BbvCI        1083  GCTGAGG              CCTCAGC(-5/-2)
BfrI          843  CTTAAG               C^TTAAG
BlnI          299  CCTAGG               C^CTAGG
BlpI         1101  GCTAAGC              GC^T[ACGT]AGC
BpmI          465  CTGGAG               CTGGAG(16/14)
Bpu1102I     1101  GCTAAGC              GC^T[ACGT]AGC
BsaWI          37  TCCGGA               [AT]^CCGG[AT]
BseAI          37  TCCGGA               T^CCGGA
BsgI         1533  CTGCAC               GTGCAG(16/14)
BsmFI         240  GTCCC                GGGAC(10/14)
BspEI          37  TCCGGA               T^CCGGA
BspMI        1031  ACCTGC               ACCTGC(4/8)
BsrFI         304  GCCGGC               [GA]^CCGG[CT]
BstAPI       1412  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1101  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        304  GCCGGC               [GA]^CCGG[CT]
DsaI          634  CCATGG               C^C[GA][CT]GG
Eam1105I      912  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          888  GAAGAG               CTCTTC(1/4)
FseI          303  GGCCGGCC             GGCCGG^CC
HgaI          131  GACGC                GACGC(5/10)
Ksp632I       888  GAAGAG               CTCTTC(1/4)
MroI           37  TCCGGA               T^CCGGA
NaeI          304  GCCGGC               GCC^GGC
NcoI          634  CCATGG               C^CATGG
NgoMIV        304  GCCGGC               G^CCGGC
PflMI         441  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1053  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1311  GAGCTC               GAGCT^C
SalI          177  GTCGAC               G^TCGAC
SfcI          355  CTGTAG               C^T[GA][CT]AG
SmaI         1156  CCCGGG               CCC^GGG
SmlI          843  CTTAAG               C^T[CT][GA]AG
SspI         1356  AATATT               AAT^ATT
StuI          706  AGGCCT               AGG^CCT
TaqI          178  TCGA                 T^CGA
TthHB8I       178  TCGA                 T^CGA
Van91I        441  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1603  TCTAGA               T^CTAGA
XmaCI        1156  CCCGGG               C^CCGGG
XmaI         1156  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
               12  CGCG                
AfaI          335  GTAC                 GT^AC
             1364  GTAC                
AlwNI         349  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              442  CAGCTGCTG           
AspI          795  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              963  GACTTTGTC           
AvaI         1096  CTCGGG               C^[CT]CG[GA]G
             1156  CCCGGG              
AvaII        1059  GGTCC                G^G[AT]CC
             1122  GGTCC               
BcnI         1156  CCCGG                CC^[GC]GG
             1157  CCGGG               
BglI          295  GCCACCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1011  GCCACTGTGGC         
BsmAI        1231  GAGAC                GTCTC(1/5)
             1752  GAGAC               
BsoBI        1096  CTCGGG               C^[CT]CG[GA]G
             1156  CCCGGG              
BstUI          10  CGCG                 CG^CG
               12  CGCG                
BstYI         769  AGATCC               [GA]^GATC[CT]
             1562  AGATCC              
CfoI           11  GCGC                 GCG^C
               43  GCGC                
FauI            8  CCCGC                CCCGC(4/6)
              161  GCGGG               
HhaI           11  GCGC                 GCG^C
               43  GCGC                
HinP1I         11  GCGC                 G^CGC
               43  GCGC                
HindIII       553  AAGCTT               A^AGCTT
             1147  AAGCTT              
MaeII          47  ACGT                 A^CGT
              148  ACGT                
MflI          769  AGATCC               [GA]^GATC[CT]
             1562  AGATCC              
MspA1I         95  CAGCTG               C[AC]G^C[GT]G
              442  CAGCTG              
MvnI           10  CGCG                 CG^CG
               12  CGCG                
NciI         1156  CCCGG                CC^[GC]GG
             1157  CCGGG               
PflFI         795  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              963  GACTTTGTC           
PleI          175  GAGTC                GAGTC(4/5)
              643  GAGTC               
PvuII          95  CAGCTG               CAG^CTG
              442  CAGCTG              
RsaI          335  GTAC                 GT^AC
             1364  GTAC                
ScaI          334  AGTACT               AGT^ACT
             1363  AGTACT              
TfiI           75  GAATC                G^A[AT]TC
             1460  GATTC               
Tth111I       795  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              963  GACTTTGTC           
XcmI         1165  CCAAAGCTCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1280  CCATGTGAGCACTGG     
XhoII         769  AGATCC               [GA]^GATC[CT]
             1562  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       BalI        BanII       BbvI        BfaI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI