##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AclI AflII AflIII AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BsaAI BsaI BsiWI BsmBI
Bsp1407I BspLU11I BspMI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 302 TCCGGA T^CCGGA
AgeI 543 ACCGGT A^CCGGT
AhdI 1182 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1597 GTGCAC G^TGCAC
ApaI 1566 GGGCCC GGGCC^C
ApaLI 1597 GTGCAC G^TGCAC
AspEI 1182 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1127 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1767 TGGCCA TGG^CCA
BamHI 753 GGATCC G^GATCC
BanI 2080 GGTGCC G^G[CT][GA]CC
BfaI 1969 CTAG C^TAG
BglII 934 AGATCT A^GATCT
BlpI 799 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 799 GCTCAGC GC^T[ACGT]AGC
BsaBI 274 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 302 TCCGGA T^CCGGA
BsgI 1502 CTGCAC GTGCAG(16/14)
BsiEI 1672 CGGCCG CG[GA][CT]^CG
BsmAI 856 GTCTC GTCTC(1/5)
BsmI 1353 GAATGC GAATGC(1/-1)
BspDI 899 ATCGAT AT^CGAT
BspEI 302 TCCGGA T^CCGGA
BspHI 2227 TCATGA T^CATGA
BssSI 851 CACGAG CACGAG(-5/-1)
BstAPI 1654 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 286 CCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 799 GCTCAGC GC^T[ACGT]AGC
ClaI 899 ATCGAT AT^CGAT
EagI 1672 CGGCCG C^GGCCG
Eam1105I 1182 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1672 CGGCCG C^GGCCG
Eco52I 1672 CGGCCG C^GGCCG
HincII 1234 GTTGAC GT[CT]^[GA]AC
HindII 1234 GTTGAC GT[CT]^[GA]AC
MaeI 1969 CTAG C^TAG
MamI 274 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1767 TGGCCA TGG^CCA
MroI 302 TCCGGA T^CCGGA
MscI 1767 TGGCCA TGG^CCA
MspA1I 802 CAGCGG C[AC]G^C[GT]G
NaeI 1670 GCCGGC GCC^GGC
NgoMIV 1670 GCCGGC G^CCGGC
NspI 846 GCATGC [GA]CATG^[CT]
PaeR7I 155 CTCGAG C^TCGAG
PflFI 1127 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 543 ACCGGT A^CCGGT
RcaI 2227 TCATGA T^CATGA
SacI 1547 GAGCTC GAGCT^C
SapI 865 GCTCTTC GCTCTTC(1/4)
ScaI 1926 AGTACT AGT^ACT
SexAI 813 ACCAGGT A^CC[AT]GGT
SmaI 549 CCCGGG CCC^GGG
SphI 846 GCATGC GCATG^C
Tth111I 1127 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 155 CTCGAG C^TCGAG
XmaCI 549 CCCGGG C^CCGGG
XmaI 549 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 719 GACGTC GACGT^C
770 GACGTC
AccI 335 GTCTAC GT^[AC][GT]AC
2491 GTCTAC
AccII 796 CGCG CG^CG
1531 CGCG
BcgI 840 CGAACCGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1608 CGAGGAGTGTGC
BsrDI 758 CATTGC GCAATG(2/0)
1147 CATTGC
BsrFI 543 ACCGGT [GA]^CCGG[CT]
1670 GCCGGC
BstUI 796 CGCG CG^CG
1531 CGCG
BstYI 753 GGATCC [GA]^GATC[CT]
934 AGATCT
Cfr10I 543 ACCGGT [GA]^CCGG[CT]
1670 GCCGGC
DraI 2147 TTTAAA TTT^AAA
2184 TTTAAA
DraII 994 AGGCCCC [GA]G^G[ACGT]CC[CT]
1565 AGGGCCC
EcoNI 184 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1844 CCTTTCTAAGG
EcoO109I 994 AGGCCCC [GA]G^G[ACGT]CC[CT]
1565 AGGGCCC
EcoRV 1 GATATC GAT^ATC
2472 GATATC
EcoT14I 117 CCATGG C^C[AT][AT]GG
378 CCATGG
HaeII 55 GGCGCT [GA]GCGC^[CT]
203 AGCGCC
MflI 753 GGATCC [GA]^GATC[CT]
934 AGATCT
MvnI 796 CGCG CG^CG
1531 CGCG
NcoI 117 CCATGG C^CATGG
378 CCATGG
PflMI 352 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2155 CCACAAGATGG
PstI 480 CTGCAG CTGCA^G
2119 CTGCAG
SmlI 20 CTTGAG C^T[CT][GA]AG
155 CTCGAG
StyI 117 CCATGG C^C[AT][AT]GG
378 CCATGG
Van91I 352 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2155 CCACAAGATGG
XhoII 753 GGATCC [GA]^GATC[CT]
934 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI