##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BclI BcnI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsiWI
BsmBI BsmI BspDI BspMI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraII DrdI
Eam1105I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 695 GTCTAC GT^[AC][GT]AC
AccIII 4 TCCGGA T^CCGGA
AcsI 1134 AAATTT [GA]^AATT[CT]
AflII 508 CTTAAG C^TTAAG
AflIII 1095 ACATGT A^C[GA][CT]GT
Aor51HI 1380 AGCGCT AGC^GCT
ApoI 1134 AAATTT [GA]^AATT[CT]
AvaI 839 CTCGAG C^[CT]CG[GA]G
BanI 950 GGTGCC G^G[CT][GA]CC
BanII 917 GAGCTC G[GA]GC[CT]^C
BbvCI 11 CCTCAGC CCTCAGC(-5/-2)
BcgI 336 GCAAGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1611 GGATAC GTATCC(6/5)
BfrI 508 CTTAAG C^TTAAG
BsaI 706 GGTCTC GGTCTC(1/5)
BseAI 4 TCCGGA T^CCGGA
BsgI 1642 CTGCAC GTGCAG(16/14)
BsmFI 276 GTCCC GGGAC(10/14)
BsoBI 839 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1146 TGTACA T^GTACA
BspEI 4 TCCGGA T^CCGGA
BspLU11I 1095 ACATGT A^CATGT
BsrDI 1331 CATTGC GCAATG(2/0)
BsrGI 1146 TGTACA T^GTACA
BssKI 279 CCAGG ^CC[ACGT]GG
BstEII 1009 GGTAACC G^GT[ACGT]ACC
BstF5I 678 GGATG GGATG(2/0)
BstNI 279 CCAGG CC^[AT]GG
BstPI 1009 GGTAACC G^GT[ACGT]ACC
BstXI 221 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1444 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 816 CCGTGG C^C[GA][CT]GG
EarI 1190 GAAGAG CTCTTC(1/4)
Eco47III 1380 AGCGCT AGC^GCT
EcoNI 1078 CCTCGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1009 GGTAACC G^GT[ACGT]ACC
EcoRII 279 CCAGG ^CC[AT]GG
FokI 678 GGATG GGATG(9/13)
HpaI 1225 GTTAAC GTT^AAC
Ksp632I 1190 GAAGAG CTCTTC(1/4)
MroI 4 TCCGGA T^CCGGA
MvaI 279 CCAGG CC^[AT]GG
NaeI 180 GCCGGC GCC^GGC
NgoMIV 180 GCCGGC G^CCGGC
PaeR7I 839 CTCGAG C^TCGAG
PflMI 1366 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 274 GAGTC GAGTC(4/5)
PstI 1417 CTGCAG CTGCA^G
SacI 917 GAGCTC GAGCT^C
SapI 1190 GAAGAGC GCTCTTC(1/4)
ScaI 51 AGTACT AGT^ACT
ScrFI 279 CCAGG CC^[ACGT]GG
SphI 1335 GCATGC GCATG^C
SspBI 1146 TGTACA T^GTACA
SspI 632 AATATT AAT^ATT
StuI 1562 AGGCCT AGG^CCT
Van91I 1366 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 913 TCTAGA T^CTAGA
XhoI 839 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 157 CGCG CG^CG
168 CGCG
AlwNI 353 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1367 CAGTTTCTG
BfaI 914 CTAG C^TAG
928 CTAG
BglII 642 AGATCT A^GATCT
686 AGATCT
BsaWI 4 TCCGGA [AT]^CCGG[AT]
959 ACCGGA
BsiEI 38 CGGCCG CG[GA][CT]^CG
45 CGGCCG
BspHI 387 TCATGA T^CATGA
552 TCATGA
BsrBI 18 GAGCGG CCGCTC(-3/-3)
172 GAGCGG
BsrFI 114 ACCGGC [GA]^CCGG[CT]
180 GCCGGC
BssSI 106 CACGAG CACGAG(-5/-1)
1447 CTCGTG
BstUI 157 CGCG CG^CG
168 CGCG
Cfr10I 114 ACCGGC [GA]^CCGG[CT]
180 GCCGGC
DraI 1138 TTTAAA TTT^AAA
1545 TTTAAA
EaeI 38 CGGCCG [CT]^GGCC[GA]
45 CGGCCG
EagI 38 CGGCCG C^GGCCG
45 CGGCCG
EclXI 38 CGGCCG C^GGCCG
45 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
45 CGGCCG
HaeII 77 AGCGCC [GA]GCGC^[CT]
1380 AGCGCT
HincII 460 GTTGAC GT[CT]^[GA]AC
1225 GTTAAC
HindII 460 GTTGAC GT[CT]^[GA]AC
1225 GTTAAC
MaeI 914 CTAG C^TAG
928 CTAG
MaeII 332 ACGT A^CGT
904 ACGT
MslI 889 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
903 CACGTTGGTG
MvnI 157 CGCG CG^CG
168 CGCG
NspI 1095 ACATGT [GA]CATG^[CT]
1335 GCATGC
PvuII 247 CAGCTG CAG^CTG
265 CAGCTG
RcaI 387 TCATGA T^CATGA
552 TCATGA
SfaNI 197 GCATC GCATC(5/9)
530 GCATC
SmlI 508 CTTAAG C^T[CT][GA]AG
839 CTCGAG
TfiI 325 GAATC G^A[AT]TC
549 GAATC
Tsp45I 788 GTGAC ^GT[GC]AC
1306 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaII BbvI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfcI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI