##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AocI ApaI AscI AspEI
AspI AviII AvrII BbvCI BcgI
BciVI BcnI BfrI BlnI BlpI
Bpu1102I BseAI BsiWI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BstAPI
BstBI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FseI FspI
HindIII KspI MfeI MluI MroI
MspA1I MunI MvnI NaeI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI Psp1406I PvuII
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 102 GACGTC GACGT^C
AccI 1300 GTATAC GT^[AC][GT]AC
AcsI 354 GAATTT [GA]^AATT[CT]
AflIII 412 ACACGT A^C[GA][CT]GT
Alw44I 233 GTGCAC G^TGCAC
Aor51HI 968 AGCGCT AGC^GCT
ApaLI 233 GTGCAC G^TGCAC
ApoI 354 GAATTT [GA]^AATT[CT]
AseI 919 ATTAAT AT^TAAT
AsnI 919 ATTAAT AT^TAAT
Asp700I 1863 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 660 CCCGAG C^[CT]CG[GA]G
BamHI 683 GGATCC G^GATCC
BbeI 21 GGCGCC GGCGC^C
BbrPI 413 CACGTG CAC^GTG
BclI 1218 TGATCA T^GATCA
BglII 1152 AGATCT A^GATCT
BsaBI 29 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1087 GGTCTC GGTCTC(1/5)
BsaWI 1340 TCCGGT [AT]^CCGG[AT]
BsgI 1023 GTGCAG GTGCAG(16/14)
BsiEI 700 CGATCG CG[GA][CT]^CG
BsmBI 3 CGTCTC CGTCTC(1/5)
BsoBI 660 CCCGAG C^[CT]CG[GA]G
Bsp1407I 998 TGTACA T^GTACA
BspHI 1091 TCATGA T^CATGA
BsrGI 998 TGTACA T^GTACA
BssSI 958 CACGAG CACGAG(-5/-1)
Bst1107I 1300 GTATAC GTA^TAC
BstEII 611 GGTGACC G^GT[ACGT]ACC
BstPI 611 GGTGACC G^GT[ACGT]ACC
BstXI 400 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1300 GTATAC GTA^TAC
DrdI 1140 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 424 CCATGG C^C[GA][CT]GG
Eco47III 968 AGCGCT AGC^GCT
EcoNI 1244 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 611 GGTGACC G^GT[ACGT]ACC
FbaI 1218 TGATCA T^GATCA
HapII 1341 CCGG C^CGG
HgaI 1168 GCGTC GACGC(5/10)
HincII 537 GTTAAC GT[CT]^[GA]AC
HindII 537 GTTAAC GT[CT]^[GA]AC
HpaI 537 GTTAAC GTT^AAC
HpaII 1341 CCGG C^CGG
KasI 21 GGCGCC G^GCGCC
MamI 29 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1341 CCGG C^CGG
NarI 21 GGCGCC GG^CGCC
NcoI 424 CCATGG C^CATGG
NspI 1296 GCATGT [GA]CATG^[CT]
PmaCI 413 CACGTG CAC^GTG
PmlI 413 CACGTG CAC^GTG
PshBI 919 ATTAAT AT^TAAT
PvuI 700 CGATCG CGAT^CG
RcaI 1091 TCATGA T^CATGA
ScaI 586 AGTACT AGT^ACT
SexAI 1812 ACCAGGT A^CC[AT]GGT
SfoI 21 GGCGCC GGC^GCC
SspBI 998 TGTACA T^GTACA
TaqI 121 TCGA T^CGA
TfiI 1863 GAATC G^A[AT]TC
Tsp509I 355 AATT ^AATT
TthHB8I 121 TCGA T^CGA
XmnI 1863 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 48 GGTACC G^GTACC
109 GGTACC
AcyI 21 GGCGCC G[GA]^CG[CT]C
102 GACGTC
Asp718I 48 GGTACC G^GTACC
109 GGTACC
BbsI 275 GAAGAC GAAGAC(2/6)
895 GAAGAC
BbvI 705 GCAGC GCAGC(8/12)
1436 GCAGC
BglI 625 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
956 GCCACGAGGGC
BpuAI 275 GAAGAC GAAGAC(2/6)
895 GAAGAC
BsaAI 236 CACGTA [CT]AC^GT[GA]
413 CACGTG
BsaHI 21 GGCGCC G[GA]^CG[CT]C
102 GACGTC
BseRI 204 GAGGAG GAGGAG(10/8)
802 GAGGAG
BsmI 1164 GAATGC GAATGC(1/-1)
776 GCATTC
BsrDI 1398 CATTGC GCAATG(2/0)
379 GCAATG
EcoT14I 424 CCATGG C^C[AT][AT]GG
1319 CCTTGG
HaeII 21 GGCGCC [GA]GCGC^[CT]
968 AGCGCT
Hin1I 21 GGCGCC G[GA]^CG[CT]C
102 GACGTC
KpnI 48 GGTACC GGTAC^C
109 GGTACC
MslI 605 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
978 CATTCATGTG
NheI 1130 GCTAGC G^CTAGC
1184 GCTAGC
PstI 532 CTGCAG CTGCA^G
1248 CTGCAG
SacI 207 GAGCTC GAGCT^C
1011 GAGCTC
SapI 429 GAAGAGC GCTCTTC(1/4)
209 GCTCTTC
SmlI 856 CTCAAG C^T[CT][GA]AG
1695 CTCAAG
StyI 424 CCATGG C^C[AT][AT]GG
1319 CCTTGG
TseI 705 GCAGC G^C[AT]GC
1436 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I Tsp45I
TspRI XcmI XhoII