##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII Alw44I AlwI AlwNI
Aor51HI ApaLI AscI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlnI BsaAI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 885 GACGCC G[GA]^CG[CT]C
AgeI 20 ACCGGT A^CCGGT
AhdI 1703 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2013 CCTCAGG CC^T[ACGT]AGG
ApaI 2018 GGGCCC GGGCC^C
AseI 620 ATTAAT AT^TAAT
AsnI 620 ATTAAT AT^TAAT
AspEI 1703 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1506 CCCGAG C^[CT]CG[GA]G
BcgI 181 CGAGATAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1128 GTATCC GTATCC(6/5)
BclI 196 TGATCA T^GATCA
BfaI 1079 CTAG C^TAG
BlpI 911 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 911 GCTAAGC GC^T[ACGT]AGC
BsaHI 885 GACGCC G[GA]^CG[CT]C
BsaWI 20 ACCGGT [AT]^CCGG[AT]
BsoBI 1506 CCCGAG C^[CT]CG[GA]G
BspDI 953 ATCGAT AT^CGAT
BsrFI 20 ACCGGT [GA]^CCGG[CT]
Bst1107I 1554 GTATAC GTA^TAC
BstZ17I 1554 GTATAC GTA^TAC
Bsu36I 2013 CCTCAGG CC^T[ACGT]AGG
CelII 911 GCTAAGC GC^T[ACGT]AGC
CfoI 841 GCGC GCG^C
Cfr10I 20 ACCGGT [GA]^CCGG[CT]
ClaI 953 ATCGAT AT^CGAT
DsaI 1033 CCGTGG C^C[GA][CT]GG
Eam1105I 1703 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2013 CCTCAGG CC^T[ACGT]AGG
EcoRI 1720 GAATTC G^AATTC
FauI 10 GCGGG CCCGC(4/6)
FbaI 196 TGATCA T^GATCA
HapII 21 CCGG C^CGG
HhaI 841 GCGC GCG^C
Hin1I 885 GACGCC G[GA]^CG[CT]C
HinP1I 841 GCGC G^CGC
HincII 815 GTCAAC GT[CT]^[GA]AC
HindII 815 GTCAAC GT[CT]^[GA]AC
HpaII 21 CCGG C^CGG
MaeI 1079 CTAG C^TAG
MslI 782 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 21 CCGG C^CGG
NdeI 2186 CATATG CA^TATG
NspI 1950 GCATGC [GA]CATG^[CT]
PinAI 20 ACCGGT A^CCGGT
PshBI 620 ATTAAT AT^TAAT
SacI 1989 GAGCTC GAGCT^C
SmlI 1727 CTTGAG C^T[CT][GA]AG
SphI 1950 GCATGC GCATG^C
StuI 84 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 763 GTCTAC GT^[AC][GT]AC
1554 GTATAC
AspHI 686 GTGCTC G[AT]GC[AT]^C
1989 GAGCTC
BanII 1989 GAGCTC G[GA]GC[CT]^C
2018 GGGCCC
BsaBI 1603 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1847 GATAAGAATC
BsiHKAI 686 GTGCTC G[AT]GC[AT]^C
1989 GAGCTC
BsmFI 112 GGGAC GGGAC(10/14)
1701 GGGAC
BspHI 1096 TCATGA T^CATGA
1329 TCATGA
BspMI 115 ACCTGC ACCTGC(4/8)
1464 ACCTGC
BstXI 522 CCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
781 CCACTTGGGTGG
EarI 925 CTCTTC CTCTTC(1/4)
2030 GAAGAG
HindIII 439 AAGCTT A^AGCTT
1145 AAGCTT
Ksp632I 925 CTCTTC CTCTTC(1/4)
2030 GAAGAG
MamI 1603 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1847 GATAAGAATC
MspA1I 768 CAGCTG C[AC]G^C[GT]G
1221 CAGCTG
PpuMI 112 GGGACCT [GA]G^G[AT]CC[CT]
2094 AGGACCT
PstI 40 CTGCAG CTGCA^G
117 CTGCAG
PvuII 768 CAGCTG CAG^CTG
1221 CAGCTG
RcaI 1096 TCATGA T^CATGA
1329 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BbsI BbvI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I