##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AclI AcsI AflII AflIII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbsI BcgI BciVI BfrI
BglI BlnI BpuAI BsiEI BsiWI
Bsp1407I BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Cfr10I CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRI
EcoRV EcoT14I FseI FspI HincII
HindII HpaI MfeI MluI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StyI
SwaI Tsp509I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 525 GGTACC G^GTACC
AccI 1436 GTCTAC GT^[AC][GT]AC
AccIII 1479 TCCGGA T^CCGGA
AocI 1704 CCTGAGG CC^T[ACGT]AGG
Aor51HI 384 AGCGCT AGC^GCT
Asp718I 525 GGTACC G^GTACC
BamHI 206 GGATCC G^GATCC
BclI 1176 TGATCA T^GATCA
BglII 1219 AGATCT A^GATCT
BlpI 94 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
BsaBI 1166 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1479 TCCGGA [AT]^CCGG[AT]
BseAI 1479 TCCGGA T^CCGGA
BsgI 617 CTGCAC GTGCAG(16/14)
BsmBI 920 CGTCTC CGTCTC(1/5)
BspDI 1457 ATCGAT AT^CGAT
BspEI 1479 TCCGGA T^CCGGA
BstEII 745 GGTGACC G^GT[ACGT]ACC
BstPI 745 GGTGACC G^GT[ACGT]ACC
Bsu36I 1704 CCTGAGG CC^T[ACGT]AGG
CelII 94 GCTGAGC GC^T[ACGT]AGC
ClaI 1457 ATCGAT AT^CGAT
DraIII 362 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 141 GAAGAG CTCTTC(1/4)
Eco47III 384 AGCGCT AGC^GCT
Eco81I 1704 CCTGAGG CC^T[ACGT]AGG
EcoO65I 745 GGTGACC G^GT[ACGT]ACC
EcoT22I 1310 ATGCAT ATGCA^T
FbaI 1176 TGATCA T^GATCA
HindIII 1474 AAGCTT A^AGCTT
KpnI 525 GGTACC GGTAC^C
Ksp632I 141 GAAGAG CTCTTC(1/4)
KspI 88 CCGCGG CCGC^GG
MamI 1166 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1479 TCCGGA T^CCGGA
MseI 1768 TTAA T^TAA
NsiI 1310 ATGCAT ATGCA^T
PflMI 1676 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1108 GAGTC GAGTC(4/5)
SacII 88 CCGCGG CCGC^GG
SapI 141 GAAGAGC GCTCTTC(1/4)
ScaI 699 AGTACT AGT^ACT
SmlI 1079 CTCAAG C^T[CT][GA]AG
StuI 1708 AGGCCT AGG^CCT
Tru9I 1768 TTAA T^TAA
Van91I 1676 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 861 CCACGCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 343 GACGTC GACGT^C
1433 GACGTC
ApaI 52 GGGCCC GGGCC^C
333 GGGCCC
AspHI 519 GTGCTC G[AT]GC[AT]^C
977 GAGCAC
AspI 896 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1226 GACCGGGTC
BbeI 9 GGCGCC GGCGC^C
1319 GGCGCC
BbrPI 365 CACGTG CAC^GTG
620 CACGTG
BbvCI 268 GCTGAGG CCTCAGC(-5/-2)
1256 GCTGAGG
BpmI 1674 CTCCAG CTGGAG(16/14)
1712 CTCCAG
BsaI 1395 GGTCTC GGTCTC(1/5)
1596 GGTCTC
BseRI 1666 CTCCTC GAGGAG(10/8)
271 GAGGAG
BsiHKAI 519 GTGCTC G[AT]GC[AT]^C
977 GAGCAC
BspMI 528 ACCTGC ACCTGC(4/8)
1440 ACCTGC
BsrDI 1660 CATTGC GCAATG(2/0)
990 GCAATG
BstAPI 1408 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1530 GCATTCCCTGC
BstXI 1453 CCACATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1608 CCAGACAGATGG
DsaI 88 CCGCGG C^C[GA][CT]GG
1195 CCGTGG
KasI 9 GGCGCC G^GCGCC
1319 GGCGCC
MboII 141 GAAGA GAAGA(8/7)
934 GAAGA
NarI 9 GGCGCC GG^CGCC
1319 GGCGCC
NspI 852 GCATGT [GA]CATG^[CT]
1308 ACATGC
PflFI 896 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1226 GACCGGGTC
PmaCI 365 CACGTG CAC^GTG
620 CACGTG
PmlI 365 CACGTG CAC^GTG
620 CACGTG
SbfI 1414 CCTGCAGG CCTGCA^GG
1536 CCTGCAGG
SfoI 9 GGCGCC GGC^GCC
1319 GGCGCC
Sse8387I 1414 CCTGCAGG CCTGCA^GG
1536 CCTGCAGG
TfiI 846 GAATC G^A[AT]TC
1206 GAATC
Tth111I 896 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1226 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BsaAI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TaqI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI