##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BciVI BcnI BglI BglII
BlnI BsaHI BsiWI BsmBI BspDI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 955 TCCGGA T^CCGGA
AflII 1364 CTTAAG C^TTAAG
Alw44I 1133 GTGCAC G^TGCAC
ApaI 1632 GGGCCC GGGCC^C
ApaLI 1133 GTGCAC G^TGCAC
Asp700I 854 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1133 GTGCAC G[AT]GC[AT]^C
AvaI 754 CCCGAG C^[CT]CG[GA]G
AvaII 1735 GGACC G^G[AT]CC
BbrPI 371 CACGTG CAC^GTG
BcgI 1208 GCAGAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 826 TGATCA T^GATCA
BfrI 1364 CTTAAG C^TTAAG
BlpI 943 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 943 GCTCAGC GC^T[ACGT]AGC
BsaAI 371 CACGTG [CT]AC^GT[GA]
BsaBI 1159 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 189 GGTCTC GGTCTC(1/5)
BsaWI 955 TCCGGA [AT]^CCGG[AT]
BseAI 955 TCCGGA T^CCGGA
BsiEI 992 CGGCCG CG[GA][CT]^CG
BsiHKAI 1133 GTGCAC G[AT]GC[AT]^C
BsoBI 754 CCCGAG C^[CT]CG[GA]G
Bsp1407I 177 TGTACA T^GTACA
BspEI 955 TCCGGA T^CCGGA
BspHI 522 TCATGA T^CATGA
BsrGI 177 TGTACA T^GTACA
CelII 943 GCTCAGC GC^T[ACGT]AGC
DsaI 279 CCATGG C^C[GA][CT]GG
EagI 992 CGGCCG C^GGCCG
EclXI 992 CGGCCG C^GGCCG
Eco52I 992 CGGCCG C^GGCCG
EcoNI 194 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 826 TGATCA T^GATCA
HaeII 85 GGCGCT [GA]GCGC^[CT]
HapII 956 CCGG C^CGG
HindIII 1437 AAGCTT A^AGCTT
HpaII 956 CCGG C^CGG
HphI 1713 GGTGA GGTGA(8/7)
MaeII 372 ACGT A^CGT
MamI 1159 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 637 CAATTG C^AATTG
MroI 955 TCCGGA T^CCGGA
MspI 956 CCGG C^CGG
MunI 637 CAATTG C^AATTG
NcoI 279 CCATGG C^CATGG
PflMI 1475 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 371 CACGTG CAC^GTG
PmlI 371 CACGTG CAC^GTG
RcaI 522 TCATGA T^CATGA
SapI 272 GAAGAGC GCTCTTC(1/4)
ScaI 1683 AGTACT AGT^ACT
SpeI 1527 ACTAGT A^CTAGT
SphI 75 GCATGC GCATG^C
SspBI 177 TGTACA T^GTACA
Van91I 1475 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1663 TCTAGA T^CTAGA
XcmI 1071 CCAATGAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 854 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 174 ACATGT A^C[GA][CT]GT
1107 ACATGT
BalI 324 TGGCCA TGG^CCA
804 TGGCCA
BanI 1115 GGCACC G^G[CT][GA]CC
1414 GGTGCC
BpmI 1473 CTCCAG CTGGAG(16/14)
104 CTGGAG
BseRI 664 CTCCTC GAGGAG(10/8)
1046 GAGGAG
BsgI 507 CTGCAC GTGCAG(16/14)
1396 GTGCAG
BsmAI 190 GTCTC GTCTC(1/5)
1421 GTCTC
BsmI 934 GAATGC GAATGC(1/-1)
872 GCATTC
BspLU11I 174 ACATGT A^CATGT
1107 ACATGT
BsrDI 1234 CATTGC GCAATG(2/0)
1730 GCAATG
BstAPI 109 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1273 GCAGCAGCTGC
BstXI 906 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1637 CCACTGATCTGG
BstYI 384 AGATCC [GA]^GATC[CT]
1140 AGATCC
CfoI 86 GCGC GCG^C
530 GCGC
DraII 1631 GGGGCCC [GA]G^G[ACGT]CC[CT]
1632 GGGCCCC
EarI 272 GAAGAG CTCTTC(1/4)
1003 GAAGAG
EcoO109I 1631 GGGGCCC [GA]G^G[ACGT]CC[CT]
1632 GGGCCCC
EcoRI 330 GAATTC G^AATTC
1485 GAATTC
FauI 1493 CCCGC CCCGC(4/6)
581 GCGGG
HhaI 86 GCGC GCG^C
530 GCGC
HinP1I 86 GCGC G^CGC
530 GCGC
Ksp632I 272 GAAGAG CTCTTC(1/4)
1003 GAAGAG
MflI 384 AGATCC [GA]^GATC[CT]
1140 AGATCC
MluNI 324 TGGCCA TGG^CCA
804 TGGCCA
MscI 324 TGGCCA TGG^CCA
804 TGGCCA
MslI 403 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1856 CACATCTGTG
MspA1I 431 CAGCTG C[AC]G^C[GT]G
1277 CAGCTG
PvuII 431 CAGCTG CAG^CTG
1277 CAGCTG
SfaNI 1716 GATGC GCATC(5/9)
148 GCATC
SmlI 44 CTCAAG C^T[CT][GA]AG
1364 CTTAAG
TaqI 818 TCGA T^CGA
1386 TCGA
TthHB8I 818 TCGA T^CGA
1386 TCGA
XhoII 384 AGATCC [GA]^GATC[CT]
1140 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI