##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstF5I BstZ17I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FokI FspI HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 268 GACGTC GACGT^C
AccI 63 GTCGAC GT^[AC][GT]AC
AfaI 992 GTAC GT^AC
AflIII 1387 ACATGT A^C[GA][CT]GT
Alw44I 1391 GTGCAC G^TGCAC
AocI 1581 CCTAAGG CC^T[ACGT]AGG
ApaI 289 GGGCCC GGGCC^C
ApaLI 1391 GTGCAC G^TGCAC
AvaI 294 CCCGGG C^[CT]CG[GA]G
BalI 221 TGGCCA TGG^CCA
BbsI 1697 GAAGAC GAAGAC(2/6)
BcgI 508 GCAAAGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 52 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 279 GCTCAGC GC^T[ACGT]AGC
BpmI 238 CTGGAG CTGGAG(16/14)
Bpu1102I 279 GCTCAGC GC^T[ACGT]AGC
BpuAI 1697 GAAGAC GAAGAC(2/6)
BseRI 1438 GAGGAG GAGGAG(10/8)
BsmBI 201 CGTCTC CGTCTC(1/5)
BsmI 513 GCATTC GAATGC(1/-1)
BsoBI 294 CCCGGG C^[CT]CG[GA]G
BspLU11I 1387 ACATGT A^CATGT
BspMI 356 ACCTGC ACCTGC(4/8)
BsrFI 254 GCCGGC [GA]^CCGG[CT]
BssSI 82 CTCGTG CACGAG(-5/-1)
BstEII 1645 GGTCACC G^GT[ACGT]ACC
BstPI 1645 GGTCACC G^GT[ACGT]ACC
BstXI 386 CCATGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1852 AGATCC [GA]^GATC[CT]
Bsu36I 1581 CCTAAGG CC^T[ACGT]AGG
CelII 279 GCTCAGC GC^T[ACGT]AGC
Cfr10I 254 GCCGGC [GA]^CCGG[CT]
Eco81I 1581 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1645 GGTCACC G^GT[ACGT]ACC
FseI 253 GGCCGGCC GGCCGG^CC
KspI 24 CCGCGG CCGC^GG
MflI 1852 AGATCC [GA]^GATC[CT]
MluNI 221 TGGCCA TGG^CCA
MscI 221 TGGCCA TGG^CCA
NaeI 254 GCCGGC GCC^GGC
NcoI 8 CCATGG C^CATGG
NdeI 371 CATATG CA^TATG
NgoMIV 254 GCCGGC G^CCGGC
PflMI 593 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1813 GAGTC GAGTC(4/5)
PpuMI 602 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 195 GACGCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1090 CAGCTG CAG^CTG
RsaI 992 GTAC GT^AC
SacII 24 CCGCGG CCGC^GG
SalI 63 GTCGAC G^TCGAC
SbfI 357 CCTGCAGG CCTGCA^GG
SmaI 294 CCCGGG CCC^GGG
Sse8387I 357 CCTGCAGG CCTGCA^GG
StuI 134 AGGCCT AGG^CCT
Van91I 593 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 227 CCATCTCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1852 AGATCC [GA]^GATC[CT]
XmaCI 294 CCCGGG C^CCGGG
XmaI 294 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 60 GGCGTC G[GA]^CG[CT]C
268 GACGTC
AlwI 430 GATCC GGATC(4/5)
1853 GATCC
AlwNI 467 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1864 CAGGTTCTG
AspHI 972 GTGCTC G[AT]GC[AT]^C
1391 GTGCAC
BanII 289 GGGCCC G[GA]GC[CT]^C
330 GAGCCC
BfaI 1051 CTAG C^TAG
1621 CTAG
BsaHI 60 GGCGTC G[GA]^CG[CT]C
268 GACGTC
BsiEI 108 CGGCCG CG[GA][CT]^CG
252 CGGCCG
BsiHKAI 972 GTGCTC G[AT]GC[AT]^C
1391 GTGCAC
DpnII 430 GATC ^GATC
1853 GATC
DraI 1000 TTTAAA TTT^AAA
1552 TTTAAA
DraII 289 GGGCCCC [GA]G^G[ACGT]CC[CT]
602 GGGTCCT
EagI 108 CGGCCG C^GGCCG
252 CGGCCG
EclXI 108 CGGCCG C^GGCCG
252 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
252 CGGCCG
EcoO109I 289 GGGCCCC [GA]G^G[ACGT]CC[CT]
602 GGGTCCT
EcoT14I 8 CCATGG C^C[AT][AT]GG
479 CCAAGG
HaeII 75 GGCGCT [GA]GCGC^[CT]
214 GGCGCT
Hin1I 60 GGCGTC G[GA]^CG[CT]C
268 GACGTC
HincII 63 GTCGAC GT[CT]^[GA]AC
838 GTTGAC
HindII 63 GTCGAC GT[CT]^[GA]AC
838 GTTGAC
MaeI 1051 CTAG C^TAG
1621 CTAG
MaeII 200 ACGT A^CGT
269 ACGT
MboI 430 GATC ^GATC
1853 GATC
NdeII 430 GATC ^GATC
1853 GATC
NspI 789 ACATGC [GA]CATG^[CT]
1387 ACATGT
PstI 337 CTGCAG CTGCA^G
358 CTGCAG
Sau3AI 430 GATC ^GATC
1853 GATC
SfaNI 1077 GCATC GCATC(5/9)
1925 GCATC
SfcI 337 CTGCAG C^T[GA][CT]AG
358 CTGCAG
StyI 8 CCATGG C^C[AT][AT]GG
479 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EarI EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I