##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI AlwI AocI
Aor51HI AscI Asp718I AspEI AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BpmI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI HaeII HgaI Hin1I
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NdeI NheI NotI NruI
NspV PacI PflMI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI SphI Sse8387I SspI
StuI SwaI Van91I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 47 CGCG CG^CG
AgeI 4 ACCGGT A^CCGGT
Alw44I 666 GTGCAC G^TGCAC
AlwNI 972 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1327 GGGCCC GGGCC^C
ApaLI 666 GTGCAC G^TGCAC
Asp700I 2202 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 666 GTGCAC G[AT]GC[AT]^C
AspI 1801 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 514 TGCGCA TGC^GCA
BanI 1473 GGCACC G^G[CT][GA]CC
BbsI 2462 GTCTTC GAAGAC(2/6)
BciVI 703 GGATAC GTATCC(6/5)
BcnI 302 CCGGG CC^[GC]GG
BglI 95 GCCCCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1551 AGATCT A^GATCT
BlpI 2549 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2549 GCTTAGC GC^T[ACGT]AGC
BpuAI 2462 GTCTTC GAAGAC(2/6)
BsaWI 4 ACCGGT [AT]^CCGG[AT]
BsgI 543 CTGCAC GTGCAG(16/14)
BsiHKAI 666 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1274 TGTACA T^GTACA
BspHI 2243 TCATGA T^CATGA
BsrGI 1274 TGTACA T^GTACA
BstUI 47 CGCG CG^CG
BstXI 798 CCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1551 AGATCT [GA]^GATC[CT]
CelII 2549 GCTTAGC GC^T[ACGT]AGC
DsaI 812 CCATGG C^C[GA][CT]GG
EcoNI 770 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 514 TGCGCA TGC^GCA
HincII 967 GTTAAC GT[CT]^[GA]AC
HindII 967 GTTAAC GT[CT]^[GA]AC
HindIII 1334 AAGCTT A^AGCTT
HpaI 967 GTTAAC GTT^AAC
MflI 1551 AGATCT [GA]^GATC[CT]
MvnI 47 CGCG CG^CG
NaeI 34 GCCGGC GCC^GGC
NciI 302 CCGGG CC^[GC]GG
NcoI 812 CCATGG C^CATGG
NgoMIV 34 GCCGGC G^CCGGC
PflFI 1801 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4 ACCGGT A^CCGGT
RcaI 2243 TCATGA T^CATGA
ScaI 2035 AGTACT AGT^ACT
SgrAI 3 CACCGGTG C[GA]^CCGG[CT]G
SpeI 983 ACTAGT A^CTAGT
SspBI 1274 TGTACA T^GTACA
Tth111I 1801 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1551 AGATCT [GA]^GATC[CT]
XmnI 2202 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 1373 ATTAAT AT^TAAT
1581 ATTAAT
AsnI 1373 ATTAAT AT^TAAT
1581 ATTAAT
AvaI 172 CTCGAG C^[CT]CG[GA]G
1645 CTCGAG
BsmAI 10 GTCTC GTCTC(1/5)
344 GTCTC
BsoBI 172 CTCGAG C^[CT]CG[GA]G
1645 CTCGAG
BsrFI 4 ACCGGT [GA]^CCGG[CT]
34 GCCGGC
Cac8I 34 GCCGGC GC[ACGT]^[ACGT]GC
1509 GCAGGC
Cfr10I 4 ACCGGT [GA]^CCGG[CT]
34 GCCGGC
EaeI 608 CGGCCA [CT]^GGCC[GA]
661 TGGCCG
EarI 614 CTCTTC CTCTTC(1/4)
727 CTCTTC
EcoT22I 133 ATGCAT ATGCA^T
1249 ATGCAT
FauI 45 CCCGC CCCGC(4/6)
650 GCGGG
Ksp632I 614 CTCTTC CTCTTC(1/4)
727 CTCTTC
MspA1I 645 CAGCTG C[AC]G^C[GT]G
972 CAGCTG
NsiI 133 ATGCAT ATGCA^T
1249 ATGCAT
NspI 1251 GCATGT [GA]CATG^[CT]
1303 ACATGC
PaeR7I 172 CTCGAG C^TCGAG
1645 CTCGAG
PpuMI 598 GGGTCCC [GA]G^G[AT]CC[CT]
1939 AGGACCT
PshBI 1373 ATTAAT AT^TAAT
1581 ATTAAT
PvuII 645 CAGCTG CAG^CTG
972 CAGCTG
SmlI 172 CTCGAG C^T[CT][GA]AG
1645 CTCGAG
Tsp45I 1431 GTGAC ^GT[GC]AC
1446 GTGAC
XhoI 172 CTCGAG C^TCGAG
1645 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AvaII BanII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI Bst1107I
BstF5I BstNI BstZ17I CfoI Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I