##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HgaI HhaI HinP1I HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1378 GACGTC GACGT^C
AciI 150 GCGG CCGC(-3/-1)
AcyI 1378 GACGTC G[GA]^CG[CT]C
AflII 1384 CTTAAG C^TTAAG
AflIII 1707 ACATGT A^C[GA][CT]GT
Alw44I 1279 GTGCAC G^TGCAC
ApaLI 1279 GTGCAC G^TGCAC
AspHI 1279 GTGCAC G[AT]GC[AT]^C
AvaII 1306 GGACC G^G[AT]CC
BcgI 843 CGAACCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1296 CTAG C^TAG
BfrI 1384 CTTAAG C^TTAAG
BmyI 1279 GTGCAC G[AGT]GC[ACT]^C
BpmI 1549 CTCCAG CTGGAG(16/14)
BsaHI 1378 GACGTC G[GA]^CG[CT]C
BsaJI 1570 CCATGG C^C[ACGT][ACGT]GG
BseRI 42 GAGGAG GAGGAG(10/8)
BsiHKAI 1279 GTGCAC G[AT]GC[AT]^C
Bsp1286I 1279 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 637 TGTACA T^GTACA
BspHI 1662 TCATGA T^CATGA
BspLU11I 1707 ACATGT A^CATGT
BsrDI 954 GCAATG GCAATG(2/0)
BsrGI 637 TGTACA T^GTACA
Cac8I 1186 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 1306 GGACC G^G[ACGT]CC
DsaI 1570 CCATGG C^C[GA][CT]GG
EaeI 1223 TGGCCG [CT]^GGCC[GA]
EcoT14I 1570 CCATGG C^C[AT][AT]GG
EcoT22I 1758 ATGCAT ATGCA^T
Hin1I 1378 GACGTC G[GA]^CG[CT]C
MaeI 1296 CTAG C^TAG
NcoI 1570 CCATGG C^CATGG
NsiI 1758 ATGCAT ATGCA^T
NspI 1707 ACATGT [GA]CATG^[CT]
PacI 1110 TTAATTAA TTAAT^TAA
PleI 909 GACTC GAGTC(4/5)
RcaI 1662 TCATGA T^CATGA
SapI 1068 GAAGAGC GCTCTTC(1/4)
Sau96I 1306 GGACC G^G[ACGT]CC
ScaI 109 AGTACT AGT^ACT
SfaNI 447 GATGC GCATC(5/9)
SspBI 637 TGTACA T^GTACA
SspI 859 AATATT AAT^ATT
StyI 1570 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 339 ATTAAT AT^TAAT
1109 ATTAAT
AsnI 339 ATTAAT AT^TAAT
1109 ATTAAT
Asp700I 319 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1005 GAAAAGATTC
BclI 1232 TGATCA T^GATCA
1493 TGATCA
BssKI 56 CCTGG ^CC[ACGT]GG
1331 CCAGG
BstNI 56 CCTGG CC^[AT]GG
1331 CCAGG
EarI 1068 GAAGAG CTCTTC(1/4)
1268 GAAGAG
EcoRI 377 GAATTC G^AATTC
414 GAATTC
EcoRII 56 CCTGG ^CC[AT]GG
1331 CCAGG
FbaI 1232 TGATCA T^GATCA
1493 TGATCA
HaeIII 920 GGCC GG^CC
1224 GGCC
HincII 248 GTTGAC GT[CT]^[GA]AC
1239 GTCAAC
HindII 248 GTTGAC GT[CT]^[GA]AC
1239 GTCAAC
HphI 189 GGTGA GGTGA(8/7)
771 GGTGA
Ksp632I 1068 GAAGAG CTCTTC(1/4)
1268 GAAGAG
MaeII 1379 ACGT A^CGT
1606 ACGT
MvaI 56 CCTGG CC^[AT]GG
1331 CCAGG
PshBI 339 ATTAAT AT^TAAT
1109 ATTAAT
ScrFI 56 CCTGG CC^[ACGT]GG
1331 CCAGG
SmlI 761 CTTGAG C^T[CT][GA]AG
1384 CTTAAG
TfiI 825 GAATC G^A[AT]TC
1010 GATTC
XcmI 563 CCAGACTGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1893 CCATAATTATAGTGG
XmnI 319 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1005 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsgI BsiYI BslI BspMI
BsrI BstF5I DdeI DpnII DraI
Fnu4HI FokI HindIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII PstI RsaI Sau3AI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI