##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstXI BstZ17I Bsu36I CelII
ClaI CpoI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 75 GGTACC G^GTACC
AcyI 114 GGCGTC G[GA]^CG[CT]C
AflII 1745 CTTAAG C^TTAAG
AlwNI 1441 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 145 GGGCCC GGGCC^C
Asp718I 75 GGTACC G^GTACC
AviII 1076 TGCGCA TGC^GCA
BalI 938 TGGCCA TGG^CCA
BanII 145 GGGCCC G[GA]GC[CT]^C
BclI 1010 TGATCA T^GATCA
BfrI 1745 CTTAAG C^TTAAG
BglI 1611 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 114 GGCGTC G[GA]^CG[CT]C
BsgI 978 CTGCAC GTGCAG(16/14)
BsiEI 201 CGGTCG CG[GA][CT]^CG
BsmI 1591 GAATGC GAATGC(1/-1)
BspMI 973 ACCTGC ACCTGC(4/8)
BsrBI 84 GAGCGG CCGCTC(-3/-3)
BsrDI 683 CATTGC GCAATG(2/0)
BsrFI 1137 ACCGGC [GA]^CCGG[CT]
BssSI 981 CACGAG CACGAG(-5/-1)
BstBI 1203 TTCGAA TT^CGAA
BstEII 789 GGTCACC G^GT[ACGT]ACC
BstPI 789 GGTCACC G^GT[ACGT]ACC
Cfr10I 1137 ACCGGC [GA]^CCGG[CT]
DraII 144 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1065 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 473 GACTCATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 938 TGGCCA [CT]^GGCC[GA]
EcoO109I 144 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 789 GGTCACC G^GT[ACGT]ACC
EcoRI 821 GAATTC G^AATTC
EcoT14I 1425 CCTTGG C^C[AT][AT]GG
FbaI 1010 TGATCA T^GATCA
FspI 1076 TGCGCA TGC^GCA
Hin1I 114 GGCGTC G[GA]^CG[CT]C
HincII 1092 GTCAAC GT[CT]^[GA]AC
HindII 1092 GTCAAC GT[CT]^[GA]AC
KpnI 75 GGTACC GGTAC^C
MaeII 480 ACGT A^CGT
MluNI 938 TGGCCA TGG^CCA
MscI 938 TGGCCA TGG^CCA
MslI 1212 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 661 ACATGC [GA]CATG^[CT]
NspV 1203 TTCGAA TT^CGAA
PaeR7I 511 CTCGAG C^TCGAG
PvuII 946 CAGCTG CAG^CTG
SfcI 1115 CTACAG C^T[GA][CT]AG
SfuI 1203 TTCGAA TT^CGAA
SmaI 148 CCCGGG CCC^GGG
SspI 1485 AATATT AAT^ATT
StyI 1425 CCTTGG C^C[AT][AT]GG
XhoI 511 CTCGAG C^TCGAG
XmaCI 148 CCCGGG C^CCGGG
XmaI 148 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AhdI 109 GACGCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1032 GACACCAGGTC
AspEI 109 GACGCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1032 GACACCAGGTC
AspHI 124 GAGCAC G[AT]GC[AT]^C
652 GTGCTC
AvaI 148 CCCGGG C^[CT]CG[GA]G
511 CTCGAG
BanI 75 GGTACC G^G[CT][GA]CC
970 GGCACC
BcnI 148 CCCGG CC^[GC]GG
149 CCGGG
BpmI 1354 CTCCAG CTGGAG(16/14)
1527 CTGGAG
BsaI 810 GGTCTC GGTCTC(1/5)
1240 GGTCTC
BsiHKAI 124 GAGCAC G[AT]GC[AT]^C
652 GTGCTC
BsoBI 148 CCCGGG C^[CT]CG[GA]G
511 CTCGAG
BstAPI 971 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1332 GCAGAAGATGC
DraI 1770 TTTAAA TTT^AAA
1921 TTTAAA
Eam1105I 109 GACGCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1032 GACACCAGGTC
HapII 149 CCGG C^CGG
1138 CCGG
HpaII 149 CCGG C^CGG
1138 CCGG
MspA1I 198 CAGCGG C[AC]G^C[GT]G
946 CAGCTG
MspI 149 CCGG C^CGG
1138 CCGG
NciI 148 CCCGG CC^[GC]GG
149 CCGGG
SapI 56 GAAGAGC GCTCTTC(1/4)
381 GCTCTTC
SexAI 364 ACCTGGT A^CC[AT]GGT
1035 ACCAGGT
SmlI 511 CTCGAG C^T[CT][GA]AG
1745 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI