##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1002 GTCTAC GT^[AC][GT]AC
AccIII 47 TCCGGA T^CCGGA
AlwNI 1512 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 104 GGCGCGCC GG^CGCGCC
Asp700I 1097 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 50 GGACC G^G[AT]CC
BanI 432 GGTGCC G^G[CT][GA]CC
BanII 1011 GAGCTC G[GA]GC[CT]^C
BbvCI 738 CCTCAGC CCTCAGC(-5/-2)
BpmI 1509 CTCCAG CTGGAG(16/14)
BsaAI 1414 TACGTA [CT]AC^GT[GA]
BsaI 63 GAGACC GGTCTC(1/5)
BseAI 47 TCCGGA T^CCGGA
BseRI 1046 CTCCTC GAGGAG(10/8)
BsgI 1497 CTGCAC GTGCAG(16/14)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsmFI 539 GGGAC GGGAC(10/14)
BspEI 47 TCCGGA T^CCGGA
BssHII 105 GCGCGC G^CGCGC
BssSI 84 CTCGTG CACGAG(-5/-1)
BstAPI 1471 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 981 CCAAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1297 AGATCC [GA]^GATC[CT]
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoRI 1136 GAATTC G^AATTC
HincII 1128 GTTAAC GT[CT]^[GA]AC
HindII 1128 GTTAAC GT[CT]^[GA]AC
HpaI 1128 GTTAAC GTT^AAC
MaeII 1415 ACGT A^CGT
MflI 1297 AGATCC [GA]^GATC[CT]
MroI 47 TCCGGA T^CCGGA
MspA1I 331 CAGCTG C[AC]G^C[GT]G
PleI 1639 GAGTC GAGTC(4/5)
PvuII 331 CAGCTG CAG^CTG
SacI 1011 GAGCTC GAGCT^C
SmlI 458 CTTGAG C^T[CT][GA]AG
SnaBI 1414 TACGTA TAC^GTA
SpeI 282 ACTAGT A^CTAGT
StuI 454 AGGCCT AGG^CCT
XhoII 1297 AGATCC [GA]^GATC[CT]
XmnI 1097 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 60 CGCG CG^CG
106 CGCG
AlwI 1080 GATCC GGATC(4/5)
1298 GATCC
BsmI 615 GAATGC GAATGC(1/-1)
1215 GAATGC
BstUI 60 CGCG CG^CG
106 CGCG
DraI 140 TTTAAA TTT^AAA
1716 TTTAAA
DsaI 436 CCATGG C^C[GA][CT]GG
1370 CCATGG
EaeI 41 CGGCCG [CT]^GGCC[GA]
78 CGGCCA
HaeII 35 AGCGCC [GA]GCGC^[CT]
571 GGCGCT
MvnI 60 CGCG CG^CG
106 CGCG
NcoI 436 CCATGG C^CATGG
1370 CCATGG
NspI 790 ACATGC [GA]CATG^[CT]
1618 GCATGT
PflMI 1511 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1696 CCAACTGTTGG
SmaI 1 CCCGGG CCC^GGG
53 CCCGGG
TfiI 816 GATTC G^A[AT]TC
1706 GATTC
Van91I 1511 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1696 CCAACTGTTGG
XcmI 436 CCATGGAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1051 CCACAAGTGTGTTGG
XmaCI 1 CCCGGG C^CCGGG
53 CCCGGG
XmaI 1 CCCGGG C^CCGGG
53 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI BbvI BcnI BfaI
BmyI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI