################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII ClaI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI FspI KpnI MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII SalI SapI SbfI SexAI SfiI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AocI 1495 CCTCAGG CC^T[ACGT]AGG Asp700I 960 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1507 GAGCTC G[AT]GC[AT]^C BamHI 1272 GGATCC G^GATCC BbsI 985 GTCTTC GAAGAC(2/6) BglI 318 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 985 GTCTTC GAAGAC(2/6) BsiHKAI 1507 GAGCTC G[AT]GC[AT]^C Bsp1407I 921 TGTACA T^GTACA BspHI 863 TCATGA T^CATGA BsrDI 1536 CATTGC GCAATG(2/0) BsrGI 921 TGTACA T^GTACA BstBI 1469 TTCGAA TT^CGAA Bsu36I 1495 CCTCAGG CC^T[ACGT]AGG CpoI 366 CGGTCCG CG^G[AT]CCG DraI 742 TTTAAA TTT^AAA EagI 537 CGGCCG C^GGCCG EclXI 537 CGGCCG C^GGCCG Eco52I 537 CGGCCG C^GGCCG Eco81I 1495 CCTCAGG CC^T[ACGT]AGG EcoNI 184 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1232 GAATTC G^AATTC HincII 1408 GTTAAC GT[CT]^[GA]AC HindII 1408 GTTAAC GT[CT]^[GA]AC HindIII 1702 AAGCTT A^AGCTT HpaI 1408 GTTAAC GTT^AAC MslI 513 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 197 CCATGG C^CATGG NspI 1248 GCATGT [GA]CATG^[CT] NspV 1469 TTCGAA TT^CGAA PpuMI 143 GGGACCC [GA]G^G[AT]CC[CT] RcaI 863 TCATGA T^CATGA RsrII 366 CGGTCCG CG^G[AT]CCG SacI 1507 GAGCTC GAGCT^C ScaI 497 AGTACT AGT^ACT SfuI 1469 TTCGAA TT^CGAA SgrAI 1437 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1459 CTTGAG C^T[CT][GA]AG SspBI 921 TGTACA T^GTACA XmnI 960 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 1232 GAATTC [GA]^AATT[CT] 1347 GAATTT ApaI 193 GGGCCC GGGCC^C 420 GGGCCC ApoI 1232 GAATTC [GA]^AATT[CT] 1347 GAATTT BclI 651 TGATCA T^GATCA 1182 TGATCA BfaI 345 CTAG C^TAG 982 CTAG BpmI 845 CTGGAG CTGGAG(16/14) 904 CTGGAG BseRI 22 CTCCTC GAGGAG(10/8) 814 GAGGAG BsiEI 537 CGGCCG CG[GA][CT]^CG 567 CGACCG BsmI 950 GAATGC GAATGC(1/-1) 1372 GCATTC BspMI 454 ACCTGC ACCTGC(4/8) 1576 ACCTGC BsrBI 550 CCGCTC CCGCTC(-3/-3) 241 GAGCGG BsrI 844 ACTGG ACTGG(1/-1) 820 CCAGT BstYI 1272 GGATCC [GA]^GATC[CT] 1481 GGATCT EarI 1490 CTCTTC CTCTTC(1/4) 151 GAAGAG FbaI 651 TGATCA T^GATCA 1182 TGATCA HinfI 589 GACTC G^A[ACGT]TC 831 GACTC Ksp632I 1490 CTCTTC CTCTTC(1/4) 151 GAAGAG MaeI 345 CTAG C^TAG 982 CTAG MflI 1272 GGATCC [GA]^GATC[CT] 1481 GGATCT PleI 589 GACTC GAGTC(4/5) 831 GACTC PstI 456 CTGCAG CTGCA^G 721 CTGCAG SmaI 104 CCCGGG CCC^GGG 322 CCCGGG Tsp45I 520 GTGAC ^GT[GC]AC 1157 GTGAC XhoII 1272 GGATCC [GA]^GATC[CT] 1481 GGATCT XmaCI 104 CCCGGG C^CCGGG 322 CCCGGG XmaI 104 CCCGGG C^CCGGG 322 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrFI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I Van91I