##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I CelII ClaI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FseI FspI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII SalI SapI SbfI
SexAI SfiI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AocI 1495 CCTCAGG CC^T[ACGT]AGG
Asp700I 960 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1507 GAGCTC G[AT]GC[AT]^C
BamHI 1272 GGATCC G^GATCC
BbsI 985 GTCTTC GAAGAC(2/6)
BglI 318 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 985 GTCTTC GAAGAC(2/6)
BsiHKAI 1507 GAGCTC G[AT]GC[AT]^C
Bsp1407I 921 TGTACA T^GTACA
BspHI 863 TCATGA T^CATGA
BsrDI 1536 CATTGC GCAATG(2/0)
BsrGI 921 TGTACA T^GTACA
BstBI 1469 TTCGAA TT^CGAA
Bsu36I 1495 CCTCAGG CC^T[ACGT]AGG
CpoI 366 CGGTCCG CG^G[AT]CCG
DraI 742 TTTAAA TTT^AAA
EagI 537 CGGCCG C^GGCCG
EclXI 537 CGGCCG C^GGCCG
Eco52I 537 CGGCCG C^GGCCG
Eco81I 1495 CCTCAGG CC^T[ACGT]AGG
EcoNI 184 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1232 GAATTC G^AATTC
HincII 1408 GTTAAC GT[CT]^[GA]AC
HindII 1408 GTTAAC GT[CT]^[GA]AC
HindIII 1702 AAGCTT A^AGCTT
HpaI 1408 GTTAAC GTT^AAC
MslI 513 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 197 CCATGG C^CATGG
NspI 1248 GCATGT [GA]CATG^[CT]
NspV 1469 TTCGAA TT^CGAA
PpuMI 143 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 863 TCATGA T^CATGA
RsrII 366 CGGTCCG CG^G[AT]CCG
SacI 1507 GAGCTC GAGCT^C
ScaI 497 AGTACT AGT^ACT
SfuI 1469 TTCGAA TT^CGAA
SgrAI 1437 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1459 CTTGAG C^T[CT][GA]AG
SspBI 921 TGTACA T^GTACA
XmnI 960 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1232 GAATTC [GA]^AATT[CT]
1347 GAATTT
ApaI 193 GGGCCC GGGCC^C
420 GGGCCC
ApoI 1232 GAATTC [GA]^AATT[CT]
1347 GAATTT
BclI 651 TGATCA T^GATCA
1182 TGATCA
BfaI 345 CTAG C^TAG
982 CTAG
BpmI 845 CTGGAG CTGGAG(16/14)
904 CTGGAG
BseRI 22 CTCCTC GAGGAG(10/8)
814 GAGGAG
BsiEI 537 CGGCCG CG[GA][CT]^CG
567 CGACCG
BsmI 950 GAATGC GAATGC(1/-1)
1372 GCATTC
BspMI 454 ACCTGC ACCTGC(4/8)
1576 ACCTGC
BsrBI 550 CCGCTC CCGCTC(-3/-3)
241 GAGCGG
BsrI 844 ACTGG ACTGG(1/-1)
820 CCAGT
BstYI 1272 GGATCC [GA]^GATC[CT]
1481 GGATCT
EarI 1490 CTCTTC CTCTTC(1/4)
151 GAAGAG
FbaI 651 TGATCA T^GATCA
1182 TGATCA
HinfI 589 GACTC G^A[ACGT]TC
831 GACTC
Ksp632I 1490 CTCTTC CTCTTC(1/4)
151 GAAGAG
MaeI 345 CTAG C^TAG
982 CTAG
MflI 1272 GGATCC [GA]^GATC[CT]
1481 GGATCT
PleI 589 GACTC GAGTC(4/5)
831 GACTC
PstI 456 CTGCAG CTGCA^G
721 CTGCAG
SmaI 104 CCCGGG CCC^GGG
322 CCCGGG
Tsp45I 520 GTGAC ^GT[GC]AC
1157 GTGAC
XhoII 1272 GGATCC [GA]^GATC[CT]
1481 GGATCT
XmaCI 104 CCCGGG C^CCGGG
322 CCCGGG
XmaI 104 CCCGGG C^CCGGG
322 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI KspI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I