##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeII MamI MluI
MluNI MroI MscI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1178 GGTACC G^GTACC
AccI 416 GTATAC GT^[AC][GT]AC
AccII 79 CGCG CG^CG
AflIII 914 ACATGT A^C[GA][CT]GT
AseI 1283 ATTAAT AT^TAAT
AsnI 1283 ATTAAT AT^TAAT
Asp718I 1178 GGTACC G^GTACC
AspHI 1428 GTGCTC G[AT]GC[AT]^C
AvaI 378 CTCGAG C^[CT]CG[GA]G
AvaII 82 GGACC G^G[AT]CC
BamHI 133 GGATCC G^GATCC
BanI 1178 GGTACC G^G[CT][GA]CC
BciVI 1075 GGATAC GTATCC(6/5)
BsaI 344 GAGACC GGTCTC(1/5)
BseRI 1158 GAGGAG GAGGAG(10/8)
BsiHKAI 1428 GTGCTC G[AT]GC[AT]^C
BsmAI 344 GAGAC GTCTC(1/5)
BsoBI 378 CTCGAG C^[CT]CG[GA]G
BspHI 1574 TCATGA T^CATGA
BspLU11I 914 ACATGT A^CATGT
Bst1107I 416 GTATAC GTA^TAC
BstAPI 112 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 79 CGCG CG^CG
BstZ17I 416 GTATAC GTA^TAC
Cac8I 1249 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 82 GGACC G^G[ACGT]CC
DraIII 1507 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 577 GAATTC G^AATTC
FauI 1651 CCCGC CCCGC(4/6)
HaeII 286 GGCGCT [GA]GCGC^[CT]
HapII 36 CCGG C^CGG
HpaII 36 CCGG C^CGG
KpnI 1178 GGTACC GGTAC^C
MfeI 322 CAATTG C^AATTG
MslI 1269 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 36 CCGG C^CGG
MunI 322 CAATTG C^AATTG
MvnI 79 CGCG CG^CG
NcoI 12 CCATGG C^CATGG
PaeR7I 378 CTCGAG C^TCGAG
PshBI 1283 ATTAAT AT^TAAT
PstI 1 CTGCAG CTGCA^G
RcaI 1574 TCATGA T^CATGA
Sau96I 82 GGACC G^G[ACGT]CC
SexAI 1508 ACCTGGT A^CC[AT]GGT
SfcI 1 CTGCAG C^T[GA][CT]AG
SpeI 991 ACTAGT A^CTAGT
SspI 860 AATATT AAT^ATT
StuI 490 AGGCCT AGG^CCT
TaqI 379 TCGA T^CGA
Tsp45I 145 GTCAC ^GT[GC]AC
TthHB8I 379 TCGA T^CGA
XbaI 1762 TCTAGA T^CTAGA
XcmI 1423 CCATTGTGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 378 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BmyI 328 GGGCAC G[AGT]GC[ACT]^C
1428 GTGCTC
BpmI 292 CTCCAG CTGGAG(16/14)
1446 CTGGAG
BsmFI 1503 GGGAC GGGAC(10/14)
664 GTCCC
Bsp1286I 328 GGGCAC G[AGT]GC[ACT]^C
1428 GTGCTC
BstYI 133 GGATCC [GA]^GATC[CT]
508 AGATCC
CfoI 6 GCGC GCG^C
287 GCGC
DsaI 12 CCATGG C^C[GA][CT]GG
1454 CCGTGG
HgaI 77 GACGC GACGC(5/10)
178 GACGC
HhaI 6 GCGC GCG^C
287 GCGC
HinP1I 6 GCGC G^CGC
287 GCGC
MflI 133 GGATCC [GA]^GATC[CT]
508 AGATCC
MspA1I 708 CAGCTG C[AC]G^C[GT]G
1227 CAGCTG
NspI 914 ACATGT [GA]CATG^[CT]
1258 GCATGT
PflMI 1183 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1732 CCAGTTTGTGG
PvuII 708 CAGCTG CAG^CTG
1227 CAGCTG
Van91I 1183 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1732 CCAGTTTGTGG
XhoII 133 GGATCC [GA]^GATC[CT]
508 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbsI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI DdeI DpnII
DraI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI